Strain Fitness in Escherichia coli ECRC62 around BNILDI_21235

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpoB and pal are separated by 9 nucleotidespal and tolB are separated by 34 nucleotidestolB and tolA are separated by 132 nucleotides BNILDI_21225: cpoB - cell division protein CpoB, at 4,265,529 to 4,266,320 cpoB BNILDI_21230: pal - peptidoglycan-associated lipoprotein Pal, at 4,266,330 to 4,266,851 pal BNILDI_21235: tolB - Tol-Pal system protein TolB, at 4,266,886 to 4,268,178 tolB BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609 tolA Position (kb) 4266 4267 4268 4269Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 4266.179 kb on + strand, within cpoBat 4266.180 kb on - strand, within cpoBat 4266.267 kb on + strandat 4266.268 kb on - strandat 4266.269 kb on + strandat 4266.269 kb on + strandat 4266.295 kb on - strandat 4266.388 kb on - strand, within palat 4266.457 kb on + strand, within palat 4266.457 kb on + strand, within palat 4266.457 kb on + strand, within palat 4266.465 kb on - strand, within palat 4266.538 kb on - strand, within palat 4266.551 kb on - strand, within palat 4266.551 kb on - strand, within palat 4266.563 kb on + strand, within palat 4266.620 kb on - strand, within palat 4266.726 kb on - strand, within palat 4267.013 kb on - strandat 4267.075 kb on + strand, within tolBat 4267.076 kb on - strand, within tolBat 4267.197 kb on + strand, within tolBat 4267.200 kb on + strand, within tolBat 4267.201 kb on - strand, within tolBat 4267.201 kb on - strand, within tolBat 4267.266 kb on + strand, within tolBat 4267.267 kb on - strand, within tolBat 4267.368 kb on - strand, within tolBat 4267.471 kb on + strand, within tolBat 4267.674 kb on - strand, within tolBat 4267.680 kb on + strand, within tolBat 4267.751 kb on + strand, within tolBat 4267.795 kb on + strand, within tolBat 4267.796 kb on - strand, within tolBat 4267.845 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.967 kb on - strand, within tolBat 4268.164 kb on + strandat 4268.181 kb on + strandat 4268.182 kb on - strandat 4268.182 kb on - strandat 4268.251 kb on + strandat 4268.252 kb on - strandat 4268.313 kb on - strandat 4268.504 kb on + strand, within tolAat 4268.505 kb on - strand, within tolAat 4268.520 kb on - strand, within tolAat 4268.520 kb on - strand, within tolAat 4268.563 kb on - strand, within tolAat 4268.595 kb on + strand, within tolAat 4268.596 kb on - strand, within tolAat 4269.150 kb on + strand, within tolA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,266,179 + cpoB BNILDI_21225 0.82 -0.2
4,266,180 - cpoB BNILDI_21225 0.82 -0.9
4,266,267 + +1.4
4,266,268 - -0.6
4,266,269 + +0.4
4,266,269 + -0.8
4,266,295 - -0.4
4,266,388 - pal BNILDI_21230 0.11 -0.5
4,266,457 + pal BNILDI_21230 0.24 -3.9
4,266,457 + pal BNILDI_21230 0.24 -1.9
4,266,457 + pal BNILDI_21230 0.24 -2.3
4,266,465 - pal BNILDI_21230 0.26 -1.2
4,266,538 - pal BNILDI_21230 0.40 -1.1
4,266,551 - pal BNILDI_21230 0.42 +0.5
4,266,551 - pal BNILDI_21230 0.42 -3.6
4,266,563 + pal BNILDI_21230 0.45 -3.1
4,266,620 - pal BNILDI_21230 0.56 -2.7
4,266,726 - pal BNILDI_21230 0.76 -1.6
4,267,013 - -2.2
4,267,075 + tolB BNILDI_21235 0.15 -1.8
4,267,076 - tolB BNILDI_21235 0.15 -4.3
4,267,197 + tolB BNILDI_21235 0.24 -2.2
4,267,200 + tolB BNILDI_21235 0.24 -2.2
4,267,201 - tolB BNILDI_21235 0.24 -1.1
4,267,201 - tolB BNILDI_21235 0.24 -2.1
4,267,266 + tolB BNILDI_21235 0.29 -1.2
4,267,267 - tolB BNILDI_21235 0.29 -0.9
4,267,368 - tolB BNILDI_21235 0.37 -4.3
4,267,471 + tolB BNILDI_21235 0.45 -3.4
4,267,674 - tolB BNILDI_21235 0.61 -1.7
4,267,680 + tolB BNILDI_21235 0.61 -2.4
4,267,751 + tolB BNILDI_21235 0.67 -1.1
4,267,795 + tolB BNILDI_21235 0.70 -2.1
4,267,796 - tolB BNILDI_21235 0.70 -0.2
4,267,845 - tolB BNILDI_21235 0.74 +0.5
4,267,883 - tolB BNILDI_21235 0.77 -3.2
4,267,883 - tolB BNILDI_21235 0.77 -3.2
4,267,883 - tolB BNILDI_21235 0.77 -3.1
4,267,967 - tolB BNILDI_21235 0.84 -2.3
4,268,164 + -2.0
4,268,181 + -2.6
4,268,182 - -0.5
4,268,182 - -0.5
4,268,251 + +0.3
4,268,252 - +0.2
4,268,313 - -0.1
4,268,504 + tolA BNILDI_21240 0.15 -6.1
4,268,505 - tolA BNILDI_21240 0.15 +2.1
4,268,520 - tolA BNILDI_21240 0.16 -3.4
4,268,520 - tolA BNILDI_21240 0.16 -5.7
4,268,563 - tolA BNILDI_21240 0.19 -2.0
4,268,595 + tolA BNILDI_21240 0.22 -0.9
4,268,596 - tolA BNILDI_21240 0.22 -1.4
4,269,150 + tolA BNILDI_21240 0.65 -2.9

Or see this region's nucleotide sequence