Strain Fitness in Escherichia coli ECRC62 around BNILDI_21230

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLys_trna and cpoB are separated by 164 nucleotidescpoB and pal are separated by 9 nucleotidespal and tolB are separated by 34 nucleotides BNILDI_21220: Lys_trna - tRNA-Lys(ttt), at 4,265,289 to 4,265,364 _trna BNILDI_21225: cpoB - cell division protein CpoB, at 4,265,529 to 4,266,320 cpoB BNILDI_21230: pal - peptidoglycan-associated lipoprotein Pal, at 4,266,330 to 4,266,851 pal BNILDI_21235: tolB - Tol-Pal system protein TolB, at 4,266,886 to 4,268,178 tolB Position (kb) 4266 4267Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4265.500 kb on + strandat 4265.501 kb on - strandat 4265.530 kb on + strandat 4265.530 kb on + strandat 4265.530 kb on + strandat 4265.531 kb on - strandat 4265.531 kb on - strandat 4265.585 kb on + strandat 4265.585 kb on + strandat 4265.602 kb on + strandat 4265.603 kb on - strandat 4265.604 kb on + strandat 4265.717 kb on - strand, within cpoBat 4265.739 kb on - strand, within cpoBat 4265.750 kb on - strand, within cpoBat 4265.754 kb on + strand, within cpoBat 4265.755 kb on - strand, within cpoBat 4265.770 kb on + strand, within cpoBat 4265.873 kb on + strand, within cpoBat 4265.885 kb on - strand, within cpoBat 4266.179 kb on + strand, within cpoBat 4266.180 kb on - strand, within cpoBat 4266.267 kb on + strandat 4266.268 kb on - strandat 4266.269 kb on + strandat 4266.269 kb on + strandat 4266.295 kb on - strandat 4266.388 kb on - strand, within palat 4266.457 kb on + strand, within palat 4266.457 kb on + strand, within palat 4266.457 kb on + strand, within palat 4266.465 kb on - strand, within palat 4266.538 kb on - strand, within palat 4266.551 kb on - strand, within palat 4266.551 kb on - strand, within palat 4266.563 kb on + strand, within palat 4266.620 kb on - strand, within palat 4266.726 kb on - strand, within palat 4267.013 kb on - strandat 4267.075 kb on + strand, within tolBat 4267.076 kb on - strand, within tolBat 4267.197 kb on + strand, within tolBat 4267.200 kb on + strand, within tolBat 4267.201 kb on - strand, within tolBat 4267.201 kb on - strand, within tolBat 4267.266 kb on + strand, within tolBat 4267.267 kb on - strand, within tolBat 4267.368 kb on - strand, within tolBat 4267.471 kb on + strand, within tolBat 4267.674 kb on - strand, within tolBat 4267.680 kb on + strand, within tolBat 4267.751 kb on + strand, within tolBat 4267.795 kb on + strand, within tolBat 4267.796 kb on - strand, within tolBat 4267.845 kb on - strand, within tolB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,265,500 + +1.7
4,265,501 - -0.4
4,265,530 + +0.5
4,265,530 + +0.5
4,265,530 + -0.3
4,265,531 - -1.9
4,265,531 - -0.7
4,265,585 + -0.4
4,265,585 + -0.5
4,265,602 + -0.5
4,265,603 - +0.8
4,265,604 + +1.3
4,265,717 - cpoB BNILDI_21225 0.24 +0.5
4,265,739 - cpoB BNILDI_21225 0.27 -1.5
4,265,750 - cpoB BNILDI_21225 0.28 +1.9
4,265,754 + cpoB BNILDI_21225 0.28 +1.2
4,265,755 - cpoB BNILDI_21225 0.29 +0.6
4,265,770 + cpoB BNILDI_21225 0.30 -0.2
4,265,873 + cpoB BNILDI_21225 0.43 +0.9
4,265,885 - cpoB BNILDI_21225 0.45 +0.1
4,266,179 + cpoB BNILDI_21225 0.82 -0.2
4,266,180 - cpoB BNILDI_21225 0.82 -0.9
4,266,267 + +1.4
4,266,268 - -0.6
4,266,269 + +0.4
4,266,269 + -0.8
4,266,295 - -0.4
4,266,388 - pal BNILDI_21230 0.11 -0.5
4,266,457 + pal BNILDI_21230 0.24 -3.9
4,266,457 + pal BNILDI_21230 0.24 -1.9
4,266,457 + pal BNILDI_21230 0.24 -2.3
4,266,465 - pal BNILDI_21230 0.26 -1.3
4,266,538 - pal BNILDI_21230 0.40 -1.1
4,266,551 - pal BNILDI_21230 0.42 +0.5
4,266,551 - pal BNILDI_21230 0.42 -3.6
4,266,563 + pal BNILDI_21230 0.45 -3.1
4,266,620 - pal BNILDI_21230 0.56 -2.7
4,266,726 - pal BNILDI_21230 0.76 -1.6
4,267,013 - -2.3
4,267,075 + tolB BNILDI_21235 0.15 -1.8
4,267,076 - tolB BNILDI_21235 0.15 -4.3
4,267,197 + tolB BNILDI_21235 0.24 -2.3
4,267,200 + tolB BNILDI_21235 0.24 -2.2
4,267,201 - tolB BNILDI_21235 0.24 -1.1
4,267,201 - tolB BNILDI_21235 0.24 -2.1
4,267,266 + tolB BNILDI_21235 0.29 -1.3
4,267,267 - tolB BNILDI_21235 0.29 -0.9
4,267,368 - tolB BNILDI_21235 0.37 -4.3
4,267,471 + tolB BNILDI_21235 0.45 -3.4
4,267,674 - tolB BNILDI_21235 0.61 -1.7
4,267,680 + tolB BNILDI_21235 0.61 -2.5
4,267,751 + tolB BNILDI_21235 0.67 -1.1
4,267,795 + tolB BNILDI_21235 0.70 -2.1
4,267,796 - tolB BNILDI_21235 0.70 -0.3
4,267,845 - tolB BNILDI_21235 0.74 +0.5

Or see this region's nucleotide sequence