Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zitB and pnuC overlap by 4 nucleotides pnuC and nadA are separated by 37 nucleotides nadA and Lys_trna are separated by 404 nucleotides Lys_trna and Lys_trna are separated by 33 nucleotides Lys_trna and Lys_trna are separated by 146 nucleotides Lys_trna and Val_trna are separated by 3 nucleotides
BNILDI_21175: zitB - CDF family zinc transporter ZitB, at 4,261,222 to 4,262,163
zitB
BNILDI_21180: pnuC - nicotinamide riboside transporter PnuC, at 4,262,160 to 4,262,879
pnuC
BNILDI_21185: nadA - quinolinate synthase NadA, at 4,262,917 to 4,263,960
nadA
BNILDI_21190: Lys_trna - tRNA-Lys(ttt), at 4,264,365 to 4,264,440
_trna
BNILDI_21195: Lys_trna - tRNA-Lys(ttt), at 4,264,474 to 4,264,549
_trna
BNILDI_21200: Lys_trna - tRNA-Lys(ttt), at 4,264,696 to 4,264,771
_trna
BNILDI_21205: Val_trna - tRNA-Val(tac), at 4,264,775 to 4,264,850
_trna
Position (kb)
4262
4263
4264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.953 kb on - strand, within zitB at 4262.249 kb on - strand, within pnuC at 4262.249 kb on - strand, within pnuC at 4262.310 kb on - strand, within pnuC at 4262.339 kb on - strand, within pnuC at 4262.342 kb on + strand, within pnuC at 4262.343 kb on - strand, within pnuC at 4262.370 kb on - strand, within pnuC at 4262.501 kb on + strand, within pnuC at 4262.624 kb on - strand, within pnuC at 4262.624 kb on - strand, within pnuC at 4262.664 kb on + strand, within pnuC at 4262.665 kb on - strand, within pnuC at 4262.738 kb on - strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.772 kb on - strand, within pnuC at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.847 kb on + strand at 4262.847 kb on + strand at 4262.847 kb on + strand at 4262.848 kb on - strand at 4262.908 kb on + strand at 4262.909 kb on - strand at 4262.912 kb on - strand at 4262.912 kb on - strand at 4262.927 kb on - strand at 4262.927 kb on - strand at 4262.932 kb on - strand at 4262.950 kb on - strand at 4263.264 kb on + strand, within nadA at 4263.264 kb on + strand, within nadA at 4263.265 kb on - strand, within nadA at 4263.265 kb on - strand, within nadA at 4263.360 kb on + strand, within nadA at 4263.380 kb on - strand, within nadA at 4263.380 kb on - strand, within nadA at 4263.546 kb on - strand, within nadA at 4263.814 kb on - strand, within nadA at 4263.815 kb on + strand, within nadA at 4263.818 kb on + strand, within nadA at 4263.818 kb on + strand, within nadA at 4263.819 kb on - strand, within nadA at 4263.952 kb on + strand at 4263.953 kb on - strand at 4264.122 kb on + strand at 4264.147 kb on + strand at 4264.869 kb on - strand at 4264.869 kb on - strand at 4264.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,261,946 + zitB BNILDI_21175 0.77 +0.1 4,261,946 + zitB BNILDI_21175 0.77 -0.0 4,261,946 + zitB BNILDI_21175 0.77 +0.0 4,261,946 + zitB BNILDI_21175 0.77 +0.3 4,261,947 - zitB BNILDI_21175 0.77 +0.2 4,261,947 - zitB BNILDI_21175 0.77 +0.4 4,261,947 - zitB BNILDI_21175 0.77 +0.8 4,261,947 - zitB BNILDI_21175 0.77 +0.9 4,261,947 - zitB BNILDI_21175 0.77 +0.4 4,261,947 - zitB BNILDI_21175 0.77 -0.1 4,261,952 + zitB BNILDI_21175 0.77 +0.3 4,261,952 + zitB BNILDI_21175 0.77 -0.7 4,261,952 + zitB BNILDI_21175 0.77 +0.8 4,261,952 + zitB BNILDI_21175 0.77 -2.2 4,261,952 + zitB BNILDI_21175 0.77 -0.3 4,261,952 + zitB BNILDI_21175 0.77 -0.5 4,261,953 - zitB BNILDI_21175 0.78 +0.5 4,262,249 - pnuC BNILDI_21180 0.12 -0.7 4,262,249 - pnuC BNILDI_21180 0.12 -0.2 4,262,310 - pnuC BNILDI_21180 0.21 +0.0 4,262,339 - pnuC BNILDI_21180 0.25 -0.3 4,262,342 + pnuC BNILDI_21180 0.25 -0.0 4,262,343 - pnuC BNILDI_21180 0.25 -1.0 4,262,370 - pnuC BNILDI_21180 0.29 -1.5 4,262,501 + pnuC BNILDI_21180 0.47 -0.1 4,262,624 - pnuC BNILDI_21180 0.64 +0.6 4,262,624 - pnuC BNILDI_21180 0.64 -1.0 4,262,664 + pnuC BNILDI_21180 0.70 +0.3 4,262,665 - pnuC BNILDI_21180 0.70 +1.1 4,262,738 - pnuC BNILDI_21180 0.80 -0.6 4,262,771 + pnuC BNILDI_21180 0.85 -0.6 4,262,771 + pnuC BNILDI_21180 0.85 -1.2 4,262,771 + pnuC BNILDI_21180 0.85 +0.3 4,262,772 - pnuC BNILDI_21180 0.85 +0.2 4,262,841 + -0.5 4,262,841 + -1.0 4,262,841 + -0.3 4,262,841 + +0.2 4,262,841 + -0.9 4,262,841 + +0.5 4,262,844 - +0.5 4,262,844 - -0.5 4,262,844 - -0.6 4,262,844 - -0.4 4,262,847 + -0.2 4,262,847 + -0.6 4,262,847 + +0.3 4,262,848 - +1.1 4,262,908 + +0.1 4,262,909 - +0.0 4,262,912 - -0.7 4,262,912 - +0.4 4,262,927 - +0.8 4,262,927 - +0.3 4,262,932 - -0.8 4,262,950 - -0.0 4,263,264 + nadA BNILDI_21185 0.33 -2.4 4,263,264 + nadA BNILDI_21185 0.33 -2.4 4,263,265 - nadA BNILDI_21185 0.33 -0.4 4,263,265 - nadA BNILDI_21185 0.33 +0.5 4,263,360 + nadA BNILDI_21185 0.42 -0.9 4,263,380 - nadA BNILDI_21185 0.44 -0.5 4,263,380 - nadA BNILDI_21185 0.44 -0.6 4,263,546 - nadA BNILDI_21185 0.60 +2.7 4,263,814 - nadA BNILDI_21185 0.86 -1.5 4,263,815 + nadA BNILDI_21185 0.86 -1.5 4,263,818 + nadA BNILDI_21185 0.86 -1.2 4,263,818 + nadA BNILDI_21185 0.86 -1.2 4,263,819 - nadA BNILDI_21185 0.86 -3.0 4,263,952 + +0.7 4,263,953 - -1.6 4,264,122 + +0.2 4,264,147 + +0.4 4,264,869 - +0.3 4,264,869 - -0.2 4,264,876 - -0.3
Or see this region's nucleotide sequence