Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rimK and nfsA are separated by 60 nucleotides nfsA and BNILDI_20605 overlap by 4 nucleotides BNILDI_20605 and ybjC overlap by 17 nucleotides ybjC and grxA are separated by 159 nucleotides
BNILDI_20595: rimK - 30S ribosomal protein S6--L-glutamate ligase, at 4,148,895 to 4,149,797
rimK
BNILDI_20600: nfsA - oxygen-insensitive NADPH nitroreductase, at 4,149,858 to 4,150,253
nfsA
BNILDI_20605: BNILDI_20605 - Nitroreductase NfsA, at 4,150,250 to 4,150,579
_20605
BNILDI_20610: ybjC - Uncharacterized protein YbjC, at 4,150,563 to 4,150,850
ybjC
BNILDI_20615: grxA - glutaredoxin 1, at 4,151,010 to 4,151,267
grxA
Position (kb)
4149
4150
4151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4148.881 kb on + strand at 4149.263 kb on - strand, within rimK at 4149.279 kb on - strand, within rimK at 4149.542 kb on - strand, within rimK at 4149.584 kb on - strand, within rimK at 4149.650 kb on - strand, within rimK at 4149.676 kb on - strand, within rimK at 4149.676 kb on - strand, within rimK at 4149.686 kb on - strand, within rimK at 4149.686 kb on - strand, within rimK at 4149.752 kb on + strand at 4149.761 kb on - strand at 4149.784 kb on - strand at 4149.840 kb on + strand at 4149.841 kb on - strand at 4149.936 kb on - strand, within nfsA at 4150.006 kb on + strand, within nfsA at 4150.040 kb on - strand, within nfsA at 4150.198 kb on + strand, within nfsA at 4150.198 kb on + strand, within nfsA at 4150.199 kb on - strand, within nfsA at 4150.199 kb on - strand, within nfsA at 4150.200 kb on + strand, within nfsA at 4150.412 kb on + strand, within BNILDI_20605 at 4150.558 kb on + strand at 4150.559 kb on - strand at 4150.607 kb on - strand, within ybjC at 4150.813 kb on - strand, within ybjC at 4150.851 kb on - strand at 4150.882 kb on + strand at 4150.897 kb on + strand at 4150.981 kb on - strand at 4150.988 kb on + strand at 4151.009 kb on - strand at 4151.112 kb on + strand, within grxA at 4151.112 kb on + strand, within grxA at 4151.113 kb on - strand, within grxA at 4151.116 kb on + strand, within grxA at 4151.117 kb on - strand, within grxA at 4151.120 kb on + strand, within grxA at 4151.121 kb on - strand, within grxA at 4151.121 kb on - strand, within grxA at 4151.152 kb on + strand, within grxA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,148,881 + -0.5 4,149,263 - rimK BNILDI_20595 0.41 +0.0 4,149,279 - rimK BNILDI_20595 0.43 -0.2 4,149,542 - rimK BNILDI_20595 0.72 -1.1 4,149,584 - rimK BNILDI_20595 0.76 +0.2 4,149,650 - rimK BNILDI_20595 0.84 -0.5 4,149,676 - rimK BNILDI_20595 0.86 -0.2 4,149,676 - rimK BNILDI_20595 0.86 -0.0 4,149,686 - rimK BNILDI_20595 0.88 -0.1 4,149,686 - rimK BNILDI_20595 0.88 +0.1 4,149,752 + -0.0 4,149,761 - +1.9 4,149,784 - +0.3 4,149,840 + -2.5 4,149,841 - -2.3 4,149,936 - nfsA BNILDI_20600 0.20 +0.8 4,150,006 + nfsA BNILDI_20600 0.37 -0.0 4,150,040 - nfsA BNILDI_20600 0.46 +1.1 4,150,198 + nfsA BNILDI_20600 0.86 -0.5 4,150,198 + nfsA BNILDI_20600 0.86 +0.5 4,150,199 - nfsA BNILDI_20600 0.86 +0.3 4,150,199 - nfsA BNILDI_20600 0.86 -0.5 4,150,200 + nfsA BNILDI_20600 0.86 +0.2 4,150,412 + BNILDI_20605 0.49 +0.3 4,150,558 + -1.0 4,150,559 - +0.2 4,150,607 - ybjC BNILDI_20610 0.15 +2.0 4,150,813 - ybjC BNILDI_20610 0.87 -0.9 4,150,851 - +0.0 4,150,882 + -1.3 4,150,897 + +0.1 4,150,981 - -1.8 4,150,988 + -0.1 4,151,009 - -0.8 4,151,112 + grxA BNILDI_20615 0.40 +0.1 4,151,112 + grxA BNILDI_20615 0.40 +0.1 4,151,113 - grxA BNILDI_20615 0.40 +0.2 4,151,116 + grxA BNILDI_20615 0.41 +0.1 4,151,117 - grxA BNILDI_20615 0.41 +0.5 4,151,120 + grxA BNILDI_20615 0.43 +0.4 4,151,121 - grxA BNILDI_20615 0.43 -0.2 4,151,121 - grxA BNILDI_20615 0.43 +0.1 4,151,152 + grxA BNILDI_20615 0.55 +0.1
Or see this region's nucleotide sequence