Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infA and Ser_trna are separated by 252 nucleotides Ser_trna and clpA are separated by 343 nucleotides clpA and clpS are separated by 30 nucleotides clpS and yljB are separated by 98 nucleotides yljB and cspD are separated by 149 nucleotides cspD and macB are separated by 72 nucleotides
BNILDI_20425: infA - translation initiation factor IF-1, at 4,115,345 to 4,115,563
infA
BNILDI_20430: Ser_trna - tRNA-Ser(gga), at 4,115,816 to 4,115,903
_trna
BNILDI_20435: clpA - ATP-dependent Clp protease ATP-binding subunit ClpA, at 4,116,247 to 4,118,523
clpA
BNILDI_20440: clpS - ATP-dependent Clp protease adapter ClpS, at 4,118,554 to 4,118,874
clpS
BNILDI_20445: yljB - Protein YljB, at 4,118,973 to 4,119,047
yljB
BNILDI_20450: cspD - cold shock-like protein CspD, at 4,119,197 to 4,119,421
cspD
BNILDI_20455: macB - macrolide ABC transporter ATP-binding protein/permease MacB, at 4,119,494 to 4,121,440
macB
Position (kb)
4116
4117
4118
4119 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4115.641 kb on + strand at 4115.642 kb on - strand at 4115.642 kb on - strand at 4115.642 kb on - strand at 4115.660 kb on - strand at 4115.688 kb on + strand at 4115.688 kb on + strand at 4115.689 kb on - strand at 4115.689 kb on - strand at 4115.690 kb on + strand at 4115.690 kb on + strand at 4115.691 kb on - strand at 4115.691 kb on - strand at 4115.725 kb on - strand at 4115.729 kb on - strand at 4115.769 kb on - strand at 4115.993 kb on - strand at 4115.993 kb on - strand at 4116.040 kb on + strand at 4116.040 kb on + strand at 4116.043 kb on + strand at 4116.063 kb on - strand at 4116.066 kb on - strand at 4116.232 kb on + strand at 4116.534 kb on + strand, within clpA at 4116.951 kb on + strand, within clpA at 4116.952 kb on - strand, within clpA at 4116.953 kb on + strand, within clpA at 4117.131 kb on + strand, within clpA at 4117.228 kb on - strand, within clpA at 4117.362 kb on - strand, within clpA at 4117.489 kb on - strand, within clpA at 4117.553 kb on + strand, within clpA at 4117.906 kb on - strand, within clpA at 4117.930 kb on - strand, within clpA at 4118.019 kb on + strand, within clpA at 4118.112 kb on + strand, within clpA at 4118.149 kb on - strand, within clpA at 4118.149 kb on - strand, within clpA at 4118.159 kb on + strand, within clpA at 4118.160 kb on - strand, within clpA at 4118.160 kb on - strand, within clpA at 4118.160 kb on - strand, within clpA at 4118.341 kb on - strand at 4118.452 kb on - strand at 4118.494 kb on + strand at 4118.495 kb on - strand at 4118.572 kb on - strand at 4118.603 kb on + strand, within clpS at 4118.717 kb on + strand, within clpS at 4118.766 kb on - strand, within clpS at 4118.790 kb on + strand, within clpS at 4118.791 kb on - strand, within clpS at 4118.792 kb on + strand, within clpS at 4118.792 kb on + strand, within clpS at 4118.792 kb on + strand, within clpS at 4118.796 kb on + strand, within clpS at 4118.796 kb on + strand, within clpS at 4118.796 kb on + strand, within clpS at 4118.796 kb on + strand, within clpS at 4118.797 kb on - strand, within clpS at 4118.882 kb on + strand at 4118.883 kb on - strand at 4118.934 kb on + strand at 4118.935 kb on - strand at 4118.963 kb on + strand at 4118.964 kb on - strand at 4118.967 kb on + strand at 4118.967 kb on + strand at 4118.967 kb on + strand at 4118.968 kb on - strand at 4118.968 kb on - strand at 4118.968 kb on - strand at 4118.972 kb on - strand at 4118.972 kb on - strand at 4119.023 kb on + strand, within yljB at 4119.048 kb on - strand at 4119.089 kb on + strand at 4119.154 kb on + strand at 4119.155 kb on - strand at 4119.164 kb on + strand at 4119.165 kb on - strand at 4119.171 kb on - strand at 4119.215 kb on - strand at 4119.287 kb on + strand, within cspD at 4119.287 kb on + strand, within cspD at 4119.385 kb on + strand, within cspD at 4119.419 kb on + strand at 4119.420 kb on - strand at 4119.441 kb on - strand at 4119.447 kb on + strand at 4119.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,115,641 + +0.2 4,115,642 - +0.2 4,115,642 - -0.6 4,115,642 - -0.5 4,115,660 - +0.1 4,115,688 + -0.4 4,115,688 + -0.5 4,115,689 - +0.2 4,115,689 - +0.3 4,115,690 + -0.2 4,115,690 + -1.0 4,115,691 - -0.1 4,115,691 - -0.3 4,115,725 - -1.5 4,115,729 - +0.9 4,115,769 - +2.8 4,115,993 - +0.6 4,115,993 - -0.4 4,116,040 + +0.5 4,116,040 + +2.4 4,116,043 + -0.1 4,116,063 - +0.2 4,116,066 - +0.9 4,116,232 + -0.2 4,116,534 + clpA BNILDI_20435 0.13 +0.7 4,116,951 + clpA BNILDI_20435 0.31 -1.1 4,116,952 - clpA BNILDI_20435 0.31 -0.2 4,116,953 + clpA BNILDI_20435 0.31 +0.1 4,117,131 + clpA BNILDI_20435 0.39 +0.1 4,117,228 - clpA BNILDI_20435 0.43 +0.7 4,117,362 - clpA BNILDI_20435 0.49 +1.2 4,117,489 - clpA BNILDI_20435 0.55 +0.1 4,117,553 + clpA BNILDI_20435 0.57 +0.8 4,117,906 - clpA BNILDI_20435 0.73 -0.5 4,117,930 - clpA BNILDI_20435 0.74 -0.3 4,118,019 + clpA BNILDI_20435 0.78 -0.6 4,118,112 + clpA BNILDI_20435 0.82 -0.7 4,118,149 - clpA BNILDI_20435 0.84 -0.6 4,118,149 - clpA BNILDI_20435 0.84 -0.7 4,118,159 + clpA BNILDI_20435 0.84 +1.0 4,118,160 - clpA BNILDI_20435 0.84 +0.6 4,118,160 - clpA BNILDI_20435 0.84 +0.2 4,118,160 - clpA BNILDI_20435 0.84 -1.7 4,118,341 - -0.6 4,118,452 - -0.1 4,118,494 + -0.1 4,118,495 - -0.0 4,118,572 - -0.3 4,118,603 + clpS BNILDI_20440 0.15 +0.9 4,118,717 + clpS BNILDI_20440 0.51 -0.4 4,118,766 - clpS BNILDI_20440 0.66 -0.3 4,118,790 + clpS BNILDI_20440 0.74 -1.0 4,118,791 - clpS BNILDI_20440 0.74 -0.9 4,118,792 + clpS BNILDI_20440 0.74 -0.1 4,118,792 + clpS BNILDI_20440 0.74 +0.8 4,118,792 + clpS BNILDI_20440 0.74 -0.6 4,118,796 + clpS BNILDI_20440 0.75 -0.1 4,118,796 + clpS BNILDI_20440 0.75 -0.8 4,118,796 + clpS BNILDI_20440 0.75 -0.1 4,118,796 + clpS BNILDI_20440 0.75 -0.2 4,118,797 - clpS BNILDI_20440 0.76 -0.3 4,118,882 + -0.8 4,118,883 - +0.0 4,118,934 + +0.4 4,118,935 - -0.1 4,118,963 + +0.1 4,118,964 - +0.6 4,118,967 + -0.0 4,118,967 + -0.3 4,118,967 + -0.5 4,118,968 - +0.8 4,118,968 - -0.1 4,118,968 - +0.4 4,118,972 - +0.4 4,118,972 - +0.3 4,119,023 + yljB BNILDI_20445 0.67 +0.0 4,119,048 - +0.1 4,119,089 + +0.1 4,119,154 + -0.8 4,119,155 - -1.6 4,119,164 + -0.1 4,119,165 - -0.6 4,119,171 - +0.1 4,119,215 - -0.5 4,119,287 + cspD BNILDI_20450 0.40 +1.9 4,119,287 + cspD BNILDI_20450 0.40 +0.4 4,119,385 + cspD BNILDI_20450 0.84 -0.6 4,119,419 + -2.4 4,119,420 - -0.8 4,119,441 - -1.3 4,119,447 + -3.9 4,119,516 - -0.2
Or see this region's nucleotide sequence