Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mukF and cmoM overlap by 4 nucleotides cmoM and elyC are separated by 135 nucleotides elyC and ycbJ overlap by 24 nucleotides
BNILDI_20230: mukF - chromosome partition protein MukF, at 4,066,033 to 4,067,355
mukF
BNILDI_20235: cmoM - tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM, at 4,067,352 to 4,068,137
cmoM
BNILDI_20240: elyC - envelope biogenesis factor ElyC, at 4,068,273 to 4,069,052
elyC
BNILDI_20245: ycbJ - Uncharacterized protein YcbJ, at 4,069,029 to 4,069,922
ycbJ
Position (kb) 4067
4068
4069 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4067.397 kb on - strand at 4067.397 kb on - strand at 4067.397 kb on - strand at 4067.397 kb on - strand at 4067.397 kb on - strand at 4067.399 kb on - strand at 4067.412 kb on - strand at 4067.415 kb on - strand at 4067.415 kb on - strand at 4067.415 kb on - strand at 4067.455 kb on - strand, within cmoM at 4067.532 kb on - strand, within cmoM at 4067.664 kb on - strand, within cmoM at 4067.698 kb on - strand, within cmoM at 4067.746 kb on - strand, within cmoM at 4067.847 kb on - strand, within cmoM at 4067.847 kb on - strand, within cmoM at 4067.926 kb on - strand, within cmoM at 4068.054 kb on - strand, within cmoM at 4068.223 kb on - strand at 4068.223 kb on - strand at 4068.245 kb on + strand at 4068.245 kb on + strand at 4068.245 kb on + strand at 4068.245 kb on + strand at 4068.245 kb on + strand at 4068.246 kb on - strand at 4068.246 kb on - strand at 4068.246 kb on - strand at 4068.247 kb on + strand at 4068.248 kb on - strand at 4068.248 kb on - strand at 4068.334 kb on + strand at 4068.335 kb on - strand at 4068.338 kb on + strand at 4068.401 kb on + strand, within elyC at 4068.402 kb on - strand, within elyC at 4068.494 kb on + strand, within elyC at 4068.494 kb on + strand, within elyC at 4068.495 kb on - strand, within elyC at 4068.495 kb on - strand, within elyC at 4068.513 kb on + strand, within elyC at 4068.513 kb on + strand, within elyC at 4068.513 kb on + strand, within elyC at 4068.537 kb on + strand, within elyC at 4068.539 kb on + strand, within elyC at 4068.661 kb on + strand, within elyC at 4068.683 kb on + strand, within elyC at 4068.684 kb on - strand, within elyC at 4068.694 kb on + strand, within elyC at 4068.694 kb on + strand, within elyC at 4068.695 kb on - strand, within elyC at 4068.741 kb on - strand, within elyC at 4069.123 kb on - strand, within ycbJ at 4069.129 kb on + strand, within ycbJ at 4069.129 kb on + strand, within ycbJ at 4069.129 kb on + strand, within ycbJ at 4069.129 kb on + strand, within ycbJ at 4069.129 kb on + strand, within ycbJ at 4069.130 kb on - strand, within ycbJ at 4069.130 kb on - strand, within ycbJ
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,067,397 - +0.7 4,067,397 - +0.9 4,067,397 - -0.3 4,067,397 - -0.4 4,067,397 - -0.2 4,067,399 - -1.5 4,067,412 - -0.2 4,067,415 - +0.1 4,067,415 - +0.5 4,067,415 - +2.2 4,067,455 - cmoM BNILDI_20235 0.13 -0.2 4,067,532 - cmoM BNILDI_20235 0.23 +0.3 4,067,664 - cmoM BNILDI_20235 0.40 +1.6 4,067,698 - cmoM BNILDI_20235 0.44 +0.9 4,067,746 - cmoM BNILDI_20235 0.50 -0.4 4,067,847 - cmoM BNILDI_20235 0.63 +0.4 4,067,847 - cmoM BNILDI_20235 0.63 -1.1 4,067,926 - cmoM BNILDI_20235 0.73 +0.1 4,068,054 - cmoM BNILDI_20235 0.89 +0.0 4,068,223 - +0.5 4,068,223 - -0.6 4,068,245 + +1.2 4,068,245 + -0.1 4,068,245 + +0.0 4,068,245 + +1.2 4,068,245 + -0.4 4,068,246 - -3.0 4,068,246 - -0.2 4,068,246 - -0.5 4,068,247 + +0.0 4,068,248 - -0.3 4,068,248 - +0.9 4,068,334 + -0.2 4,068,335 - +0.6 4,068,338 + +0.6 4,068,401 + elyC BNILDI_20240 0.16 +0.7 4,068,402 - elyC BNILDI_20240 0.17 -1.0 4,068,494 + elyC BNILDI_20240 0.28 -0.2 4,068,494 + elyC BNILDI_20240 0.28 +0.3 4,068,495 - elyC BNILDI_20240 0.28 -2.3 4,068,495 - elyC BNILDI_20240 0.28 +0.6 4,068,513 + elyC BNILDI_20240 0.31 +0.8 4,068,513 + elyC BNILDI_20240 0.31 +1.0 4,068,513 + elyC BNILDI_20240 0.31 -0.9 4,068,537 + elyC BNILDI_20240 0.34 +0.6 4,068,539 + elyC BNILDI_20240 0.34 +0.1 4,068,661 + elyC BNILDI_20240 0.50 +0.0 4,068,683 + elyC BNILDI_20240 0.53 -1.0 4,068,684 - elyC BNILDI_20240 0.53 -0.9 4,068,694 + elyC BNILDI_20240 0.54 +0.6 4,068,694 + elyC BNILDI_20240 0.54 -0.0 4,068,695 - elyC BNILDI_20240 0.54 +0.3 4,068,741 - elyC BNILDI_20240 0.60 +0.6 4,069,123 - ycbJ BNILDI_20245 0.11 +0.0 4,069,129 + ycbJ BNILDI_20245 0.11 +0.0 4,069,129 + ycbJ BNILDI_20245 0.11 +0.4 4,069,129 + ycbJ BNILDI_20245 0.11 +0.3 4,069,129 + ycbJ BNILDI_20245 0.11 -1.0 4,069,129 + ycbJ BNILDI_20245 0.11 -0.0 4,069,130 - ycbJ BNILDI_20245 0.11 -0.2 4,069,130 - ycbJ BNILDI_20245 0.11 -1.2
Or see this region's nucleotide sequence