Strain Fitness in Escherichia coli ECRC62 around BNILDI_20140

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_20130 and insB are separated by 128 nucleotidesinsB and BNILDI_20140 are separated by 109 nucleotidesBNILDI_20140 and ssuE are separated by 355 nucleotidesssuE and ssuA overlap by 8 nucleotides BNILDI_20130: BNILDI_20130 - HTH-Tnp-IS1 domain-containing protein, at 4,042,509 to 4,042,568 _20130 BNILDI_20135: insB - Transposase and inactivated derivatives, IS1 family, at 4,042,697 to 4,042,990 insB BNILDI_20140: BNILDI_20140 - fimbrial protein, at 4,043,100 to 4,043,642 _20140 BNILDI_20145: ssuE - NADPH-dependent FMN reductase, at 4,043,998 to 4,044,573 ssuE BNILDI_20150: ssuA - aliphatic sulfonate ABC transporter substrate-binding protein SsuA, at 4,044,566 to 4,045,525 ssuA Position (kb) 4043 4044Strain fitness (log2 ratio) -1 0 1 2 3at 4042.241 kb on - strandat 4042.268 kb on - strandat 4043.022 kb on - strandat 4043.038 kb on + strandat 4043.062 kb on - strandat 4043.506 kb on - strand, within BNILDI_20140at 4043.528 kb on + strand, within BNILDI_20140at 4043.534 kb on + strand, within BNILDI_20140at 4043.535 kb on - strand, within BNILDI_20140at 4043.614 kb on - strandat 4043.615 kb on + strandat 4043.987 kb on - strandat 4043.998 kb on - strandat 4044.061 kb on + strand, within ssuEat 4044.226 kb on + strand, within ssuEat 4044.226 kb on + strand, within ssuEat 4044.428 kb on + strand, within ssuEat 4044.429 kb on - strand, within ssuEat 4044.571 kb on + strandat 4044.572 kb on - strandat 4044.581 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,042,241 - -0.5
4,042,268 - -0.3
4,043,022 - -0.1
4,043,038 + -0.7
4,043,062 - +0.1
4,043,506 - BNILDI_20140 0.75 -1.7
4,043,528 + BNILDI_20140 0.79 +0.8
4,043,534 + BNILDI_20140 0.80 -0.5
4,043,535 - BNILDI_20140 0.80 +0.6
4,043,614 - -0.1
4,043,615 + +0.1
4,043,987 - +3.0
4,043,998 - +0.1
4,044,061 + ssuE BNILDI_20145 0.11 -0.2
4,044,226 + ssuE BNILDI_20145 0.40 -0.1
4,044,226 + ssuE BNILDI_20145 0.40 -0.4
4,044,428 + ssuE BNILDI_20145 0.75 +0.1
4,044,429 - ssuE BNILDI_20145 0.75 -1.0
4,044,571 + +0.3
4,044,572 - +0.3
4,044,581 - +0.3

Or see this region's nucleotide sequence