Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_19855 and hyaE overlap by 4 nucleotides hyaE and hybD overlap by 4 nucleotides hybD and hyaC overlap by 4 nucleotides hyaC and hyaB are separated by 18 nucleotides
BNILDI_19855: BNILDI_19855 - hydrogenase expression/formation protein, at 3,986,594 to 3,987,451
_19855
BNILDI_19860: hyaE - hydrogenase-1 operon protein HyaE, at 3,987,448 to 3,987,846
hyaE
BNILDI_19865: hybD - hydrogenase 1 maturation protease, at 3,987,843 to 3,988,430
hybD
BNILDI_19870: hyaC - Ni/Fe-hydrogenase b-type cytochrome subunit, at 3,988,427 to 3,989,134
hyaC
BNILDI_19875: hyaB - Ni/Fe-hydrogenase large subunit, at 3,989,153 to 3,990,946
hyaB
Position (kb)
3987
3988
3989 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3986.918 kb on + strand, within BNILDI_19855 at 3986.918 kb on + strand, within BNILDI_19855 at 3986.918 kb on + strand, within BNILDI_19855 at 3986.918 kb on + strand, within BNILDI_19855 at 3986.919 kb on - strand, within BNILDI_19855 at 3987.197 kb on - strand, within BNILDI_19855 at 3987.496 kb on + strand, within hyaE at 3987.497 kb on - strand, within hyaE at 3987.519 kb on + strand, within hyaE at 3987.519 kb on + strand, within hyaE at 3987.559 kb on + strand, within hyaE at 3987.642 kb on + strand, within hyaE at 3987.642 kb on + strand, within hyaE at 3987.642 kb on + strand, within hyaE at 3987.642 kb on + strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.643 kb on - strand, within hyaE at 3987.645 kb on - strand, within hyaE at 3987.727 kb on + strand, within hyaE at 3987.728 kb on - strand, within hyaE at 3987.753 kb on + strand, within hyaE at 3987.920 kb on - strand, within hybD at 3987.920 kb on - strand, within hybD at 3988.082 kb on + strand, within hybD at 3988.083 kb on - strand, within hybD at 3988.083 kb on - strand, within hybD at 3988.083 kb on - strand, within hybD at 3988.106 kb on + strand, within hybD at 3988.271 kb on - strand, within hybD at 3988.319 kb on - strand, within hybD at 3988.342 kb on + strand, within hybD at 3988.444 kb on + strand at 3988.444 kb on + strand at 3988.445 kb on - strand at 3988.445 kb on - strand at 3988.445 kb on - strand at 3988.448 kb on + strand at 3988.449 kb on - strand at 3988.536 kb on + strand, within hyaC at 3988.536 kb on + strand, within hyaC at 3988.660 kb on + strand, within hyaC at 3988.661 kb on - strand, within hyaC at 3988.665 kb on + strand, within hyaC at 3988.683 kb on + strand, within hyaC at 3988.767 kb on - strand, within hyaC at 3988.767 kb on - strand, within hyaC at 3988.859 kb on - strand, within hyaC at 3988.971 kb on + strand, within hyaC at 3988.971 kb on + strand, within hyaC at 3988.971 kb on + strand, within hyaC at 3988.971 kb on + strand, within hyaC at 3988.972 kb on - strand, within hyaC at 3988.972 kb on - strand, within hyaC at 3988.980 kb on + strand, within hyaC at 3988.980 kb on + strand, within hyaC at 3988.980 kb on + strand, within hyaC at 3988.981 kb on - strand, within hyaC at 3989.026 kb on - strand, within hyaC at 3989.055 kb on - strand, within hyaC at 3989.055 kb on - strand, within hyaC at 3989.243 kb on - strand at 3989.305 kb on - strand at 3989.371 kb on - strand, within hyaB at 3989.371 kb on - strand, within hyaB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,986,918 + BNILDI_19855 0.38 +0.0 3,986,918 + BNILDI_19855 0.38 +0.3 3,986,918 + BNILDI_19855 0.38 -0.1 3,986,918 + BNILDI_19855 0.38 +2.2 3,986,919 - BNILDI_19855 0.38 +0.7 3,987,197 - BNILDI_19855 0.70 -0.1 3,987,496 + hyaE BNILDI_19860 0.12 +0.4 3,987,497 - hyaE BNILDI_19860 0.12 +0.5 3,987,519 + hyaE BNILDI_19860 0.18 -0.1 3,987,519 + hyaE BNILDI_19860 0.18 -0.1 3,987,559 + hyaE BNILDI_19860 0.28 +1.5 3,987,642 + hyaE BNILDI_19860 0.49 -2.1 3,987,642 + hyaE BNILDI_19860 0.49 +0.6 3,987,642 + hyaE BNILDI_19860 0.49 -0.8 3,987,642 + hyaE BNILDI_19860 0.49 +0.0 3,987,643 - hyaE BNILDI_19860 0.49 -0.1 3,987,643 - hyaE BNILDI_19860 0.49 +0.4 3,987,643 - hyaE BNILDI_19860 0.49 +0.1 3,987,643 - hyaE BNILDI_19860 0.49 -0.3 3,987,643 - hyaE BNILDI_19860 0.49 -1.3 3,987,643 - hyaE BNILDI_19860 0.49 -0.4 3,987,645 - hyaE BNILDI_19860 0.49 +0.4 3,987,727 + hyaE BNILDI_19860 0.70 +0.2 3,987,728 - hyaE BNILDI_19860 0.70 -0.0 3,987,753 + hyaE BNILDI_19860 0.76 +0.3 3,987,920 - hybD BNILDI_19865 0.13 -0.6 3,987,920 - hybD BNILDI_19865 0.13 +0.0 3,988,082 + hybD BNILDI_19865 0.41 -0.8 3,988,083 - hybD BNILDI_19865 0.41 -0.1 3,988,083 - hybD BNILDI_19865 0.41 -0.1 3,988,083 - hybD BNILDI_19865 0.41 -1.7 3,988,106 + hybD BNILDI_19865 0.45 +0.2 3,988,271 - hybD BNILDI_19865 0.73 +0.3 3,988,319 - hybD BNILDI_19865 0.81 -1.4 3,988,342 + hybD BNILDI_19865 0.85 -0.0 3,988,444 + -0.3 3,988,444 + +0.3 3,988,445 - +0.1 3,988,445 - +0.2 3,988,445 - -0.3 3,988,448 + +0.0 3,988,449 - +1.5 3,988,536 + hyaC BNILDI_19870 0.15 +0.3 3,988,536 + hyaC BNILDI_19870 0.15 +1.1 3,988,660 + hyaC BNILDI_19870 0.33 +0.0 3,988,661 - hyaC BNILDI_19870 0.33 -0.2 3,988,665 + hyaC BNILDI_19870 0.34 -0.7 3,988,683 + hyaC BNILDI_19870 0.36 -0.5 3,988,767 - hyaC BNILDI_19870 0.48 -0.4 3,988,767 - hyaC BNILDI_19870 0.48 +0.2 3,988,859 - hyaC BNILDI_19870 0.61 +0.5 3,988,971 + hyaC BNILDI_19870 0.77 -0.7 3,988,971 + hyaC BNILDI_19870 0.77 +0.2 3,988,971 + hyaC BNILDI_19870 0.77 -0.7 3,988,971 + hyaC BNILDI_19870 0.77 -0.6 3,988,972 - hyaC BNILDI_19870 0.77 -1.2 3,988,972 - hyaC BNILDI_19870 0.77 +0.2 3,988,980 + hyaC BNILDI_19870 0.78 -0.1 3,988,980 + hyaC BNILDI_19870 0.78 +0.7 3,988,980 + hyaC BNILDI_19870 0.78 +0.1 3,988,981 - hyaC BNILDI_19870 0.78 +0.2 3,989,026 - hyaC BNILDI_19870 0.85 +0.1 3,989,055 - hyaC BNILDI_19870 0.89 -1.5 3,989,055 - hyaC BNILDI_19870 0.89 -0.5 3,989,243 - -0.6 3,989,305 - -0.3 3,989,371 - hyaB BNILDI_19875 0.12 +0.4 3,989,371 - hyaB BNILDI_19875 0.12 +0.8
Or see this region's nucleotide sequence