Strain Fitness in Escherichia coli ECRC62 around BNILDI_19705

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrutD and rutE are separated by 9 nucleotidesrutE and rutF are separated by 10 nucleotidesrutF and rutG are separated by 20 nucleotides BNILDI_19695: rutD - pyrimidine utilization protein D, at 3,953,512 to 3,954,312 rutD BNILDI_19700: rutE - malonic semialdehyde reductase, at 3,954,322 to 3,954,912 rutE BNILDI_19705: rutF - malonic semialdehyde reductase, at 3,954,923 to 3,955,417 rutF BNILDI_19710: rutG - pyrimidine utilization transport protein G, at 3,955,438 to 3,956,766 rutG Position (kb) 3954 3955 3956Strain fitness (log2 ratio) -2 -1 0 1at 3954.057 kb on + strand, within rutDat 3954.263 kb on + strandat 3954.287 kb on + strandat 3954.287 kb on + strandat 3954.287 kb on + strandat 3954.287 kb on + strandat 3954.321 kb on + strandat 3954.500 kb on + strand, within rutEat 3954.617 kb on + strand, within rutEat 3954.652 kb on - strand, within rutEat 3954.922 kb on + strandat 3954.923 kb on - strandat 3954.930 kb on + strandat 3954.930 kb on + strandat 3954.930 kb on + strandat 3954.930 kb on + strandat 3954.930 kb on + strandat 3954.931 kb on - strandat 3955.069 kb on - strand, within rutFat 3955.470 kb on - strandat 3955.625 kb on + strand, within rutGat 3955.659 kb on + strand, within rutGat 3955.804 kb on + strand, within rutGat 3955.807 kb on - strand, within rutGat 3956.045 kb on + strand, within rutGat 3956.045 kb on + strand, within rutGat 3956.045 kb on + strand, within rutGat 3956.046 kb on - strand, within rutGat 3956.087 kb on + strand, within rutGat 3956.169 kb on + strand, within rutGat 3956.169 kb on + strand, within rutGat 3956.169 kb on + strand, within rutGat 3956.172 kb on + strand, within rutGat 3956.228 kb on + strand, within rutGat 3956.229 kb on - strand, within rutGat 3956.278 kb on + strand, within rutGat 3956.279 kb on - strand, within rutGat 3956.279 kb on - strand, within rutGat 3956.280 kb on + strand, within rutGat 3956.357 kb on - strand, within rutG

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,954,057 + rutD BNILDI_19695 0.68 +0.3
3,954,263 + -0.2
3,954,287 + +0.3
3,954,287 + -0.6
3,954,287 + -0.2
3,954,287 + -0.8
3,954,321 + -1.3
3,954,500 + rutE BNILDI_19700 0.30 +0.3
3,954,617 + rutE BNILDI_19700 0.50 +1.0
3,954,652 - rutE BNILDI_19700 0.56 -0.5
3,954,922 + -0.5
3,954,923 - +0.2
3,954,930 + -1.2
3,954,930 + +1.7
3,954,930 + -0.0
3,954,930 + +0.3
3,954,930 + +0.8
3,954,931 - -1.6
3,955,069 - rutF BNILDI_19705 0.29 -1.0
3,955,470 - +0.8
3,955,625 + rutG BNILDI_19710 0.14 -0.1
3,955,659 + rutG BNILDI_19710 0.17 -2.3
3,955,804 + rutG BNILDI_19710 0.28 +0.3
3,955,807 - rutG BNILDI_19710 0.28 +0.2
3,956,045 + rutG BNILDI_19710 0.46 -0.2
3,956,045 + rutG BNILDI_19710 0.46 -0.4
3,956,045 + rutG BNILDI_19710 0.46 -0.1
3,956,046 - rutG BNILDI_19710 0.46 +0.1
3,956,087 + rutG BNILDI_19710 0.49 +0.8
3,956,169 + rutG BNILDI_19710 0.55 -0.2
3,956,169 + rutG BNILDI_19710 0.55 -1.2
3,956,169 + rutG BNILDI_19710 0.55 +0.2
3,956,172 + rutG BNILDI_19710 0.55 +0.3
3,956,228 + rutG BNILDI_19710 0.59 +0.1
3,956,229 - rutG BNILDI_19710 0.60 +0.8
3,956,278 + rutG BNILDI_19710 0.63 -0.5
3,956,279 - rutG BNILDI_19710 0.63 -2.1
3,956,279 - rutG BNILDI_19710 0.63 +0.4
3,956,280 + rutG BNILDI_19710 0.63 +0.2
3,956,357 - rutG BNILDI_19710 0.69 +0.1

Or see this region's nucleotide sequence