Strain Fitness in Escherichia coli ECRC62 around BNILDI_18475

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpB are separated by 11 nucleotidestrpB and trpA overlap by 1 nucleotides BNILDI_18470: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 3,721,009 to 3,722,367 trpD BNILDI_18475: trpB - tryptophan synthase subunit beta, at 3,722,379 to 3,723,572 trpB BNILDI_18480: trpA - tryptophan synthase subunit alpha, at 3,723,572 to 3,724,378 trpA Position (kb) 3722 3723 3724Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3721.379 kb on - strand, within trpDat 3721.394 kb on - strand, within trpDat 3721.412 kb on + strand, within trpDat 3721.413 kb on - strand, within trpDat 3721.413 kb on - strand, within trpDat 3721.413 kb on - strand, within trpDat 3721.415 kb on + strand, within trpDat 3721.415 kb on + strand, within trpDat 3721.662 kb on + strand, within trpDat 3721.662 kb on + strand, within trpDat 3721.662 kb on + strand, within trpDat 3721.662 kb on + strand, within trpDat 3721.666 kb on + strand, within trpDat 3721.667 kb on - strand, within trpDat 3721.780 kb on - strand, within trpDat 3721.784 kb on + strand, within trpDat 3721.784 kb on + strand, within trpDat 3721.785 kb on - strand, within trpDat 3721.785 kb on - strand, within trpDat 3721.785 kb on - strand, within trpDat 3721.823 kb on - strand, within trpDat 3721.841 kb on - strand, within trpDat 3721.927 kb on + strand, within trpDat 3721.927 kb on + strand, within trpDat 3721.977 kb on + strand, within trpDat 3722.029 kb on + strand, within trpDat 3722.030 kb on - strand, within trpDat 3722.037 kb on + strand, within trpDat 3722.135 kb on - strand, within trpDat 3722.141 kb on + strand, within trpDat 3722.186 kb on + strand, within trpDat 3722.189 kb on + strand, within trpDat 3722.189 kb on + strand, within trpDat 3722.189 kb on + strand, within trpDat 3722.190 kb on - strand, within trpDat 3722.231 kb on + strand, within trpDat 3722.231 kb on + strand, within trpDat 3722.241 kb on + strandat 3722.242 kb on - strandat 3722.287 kb on - strandat 3722.362 kb on + strandat 3722.362 kb on + strandat 3722.362 kb on + strandat 3722.363 kb on - strandat 3722.363 kb on - strandat 3722.389 kb on + strandat 3722.390 kb on - strandat 3722.424 kb on + strandat 3722.424 kb on + strandat 3722.425 kb on - strandat 3722.425 kb on - strandat 3722.574 kb on - strand, within trpBat 3722.598 kb on + strand, within trpBat 3722.599 kb on - strand, within trpBat 3722.643 kb on - strand, within trpBat 3722.771 kb on + strand, within trpBat 3722.775 kb on + strand, within trpBat 3722.776 kb on - strand, within trpBat 3722.777 kb on + strand, within trpBat 3722.822 kb on + strand, within trpBat 3722.888 kb on + strand, within trpBat 3722.935 kb on - strand, within trpBat 3722.936 kb on + strand, within trpBat 3722.936 kb on + strand, within trpBat 3722.936 kb on + strand, within trpBat 3722.936 kb on + strand, within trpBat 3722.937 kb on - strand, within trpBat 3723.213 kb on + strand, within trpBat 3723.213 kb on + strand, within trpBat 3723.214 kb on - strand, within trpBat 3723.214 kb on - strand, within trpBat 3723.348 kb on + strand, within trpBat 3723.348 kb on + strand, within trpBat 3723.490 kb on + strandat 3723.490 kb on + strandat 3723.680 kb on - strand, within trpAat 3723.691 kb on - strand, within trpAat 3723.720 kb on + strand, within trpAat 3723.721 kb on - strand, within trpAat 3723.724 kb on + strand, within trpAat 3723.875 kb on + strand, within trpAat 3723.875 kb on + strand, within trpAat 3723.892 kb on + strand, within trpAat 3723.893 kb on - strand, within trpAat 3723.915 kb on - strand, within trpAat 3724.009 kb on + strand, within trpAat 3724.010 kb on - strand, within trpAat 3724.021 kb on + strand, within trpAat 3724.021 kb on + strand, within trpAat 3724.021 kb on + strand, within trpAat 3724.021 kb on + strand, within trpAat 3724.022 kb on - strand, within trpAat 3724.077 kb on - strand, within trpAat 3724.094 kb on + strand, within trpAat 3724.094 kb on + strand, within trpAat 3724.094 kb on + strand, within trpAat 3724.459 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,721,379 - trpD BNILDI_18470 0.27 -3.4
3,721,394 - trpD BNILDI_18470 0.28 -1.6
3,721,412 + trpD BNILDI_18470 0.30 -2.7
3,721,413 - trpD BNILDI_18470 0.30 -3.8
3,721,413 - trpD BNILDI_18470 0.30 -1.8
3,721,413 - trpD BNILDI_18470 0.30 -1.6
3,721,415 + trpD BNILDI_18470 0.30 -3.1
3,721,415 + trpD BNILDI_18470 0.30 -2.3
3,721,662 + trpD BNILDI_18470 0.48 -0.6
3,721,662 + trpD BNILDI_18470 0.48 -3.8
3,721,662 + trpD BNILDI_18470 0.48 -2.3
3,721,662 + trpD BNILDI_18470 0.48 -2.3
3,721,666 + trpD BNILDI_18470 0.48 -5.0
3,721,667 - trpD BNILDI_18470 0.48 -4.6
3,721,780 - trpD BNILDI_18470 0.57 -1.7
3,721,784 + trpD BNILDI_18470 0.57 -3.1
3,721,784 + trpD BNILDI_18470 0.57 -0.4
3,721,785 - trpD BNILDI_18470 0.57 -2.3
3,721,785 - trpD BNILDI_18470 0.57 -2.0
3,721,785 - trpD BNILDI_18470 0.57 -3.2
3,721,823 - trpD BNILDI_18470 0.60 -1.4
3,721,841 - trpD BNILDI_18470 0.61 -2.9
3,721,927 + trpD BNILDI_18470 0.68 -5.7
3,721,927 + trpD BNILDI_18470 0.68 -3.5
3,721,977 + trpD BNILDI_18470 0.71 -4.0
3,722,029 + trpD BNILDI_18470 0.75 -4.8
3,722,030 - trpD BNILDI_18470 0.75 -3.6
3,722,037 + trpD BNILDI_18470 0.76 -2.4
3,722,135 - trpD BNILDI_18470 0.83 -2.1
3,722,141 + trpD BNILDI_18470 0.83 -4.8
3,722,186 + trpD BNILDI_18470 0.87 -4.1
3,722,189 + trpD BNILDI_18470 0.87 -3.8
3,722,189 + trpD BNILDI_18470 0.87 -1.3
3,722,189 + trpD BNILDI_18470 0.87 -4.8
3,722,190 - trpD BNILDI_18470 0.87 -1.6
3,722,231 + trpD BNILDI_18470 0.90 -3.7
3,722,231 + trpD BNILDI_18470 0.90 -2.7
3,722,241 + -1.2
3,722,242 - -3.7
3,722,287 - -0.4
3,722,362 + -0.4
3,722,362 + +0.2
3,722,362 + -0.9
3,722,363 - +0.0
3,722,363 - -2.3
3,722,389 + -3.9
3,722,390 - -3.7
3,722,424 + -3.7
3,722,424 + -4.2
3,722,425 - -2.3
3,722,425 - -2.8
3,722,574 - trpB BNILDI_18475 0.16 -5.4
3,722,598 + trpB BNILDI_18475 0.18 -3.1
3,722,599 - trpB BNILDI_18475 0.18 -2.5
3,722,643 - trpB BNILDI_18475 0.22 -5.2
3,722,771 + trpB BNILDI_18475 0.33 -2.9
3,722,775 + trpB BNILDI_18475 0.33 -1.3
3,722,776 - trpB BNILDI_18475 0.33 -4.7
3,722,777 + trpB BNILDI_18475 0.33 -5.2
3,722,822 + trpB BNILDI_18475 0.37 -3.7
3,722,888 + trpB BNILDI_18475 0.43 -0.8
3,722,935 - trpB BNILDI_18475 0.47 -5.4
3,722,936 + trpB BNILDI_18475 0.47 -3.7
3,722,936 + trpB BNILDI_18475 0.47 -4.7
3,722,936 + trpB BNILDI_18475 0.47 -4.8
3,722,936 + trpB BNILDI_18475 0.47 -3.6
3,722,937 - trpB BNILDI_18475 0.47 -0.1
3,723,213 + trpB BNILDI_18475 0.70 -5.6
3,723,213 + trpB BNILDI_18475 0.70 -1.1
3,723,214 - trpB BNILDI_18475 0.70 -1.4
3,723,214 - trpB BNILDI_18475 0.70 -3.9
3,723,348 + trpB BNILDI_18475 0.81 -4.5
3,723,348 + trpB BNILDI_18475 0.81 -5.4
3,723,490 + -2.5
3,723,490 + -2.0
3,723,680 - trpA BNILDI_18480 0.13 -3.5
3,723,691 - trpA BNILDI_18480 0.15 -2.3
3,723,720 + trpA BNILDI_18480 0.18 -4.3
3,723,721 - trpA BNILDI_18480 0.18 -4.2
3,723,724 + trpA BNILDI_18480 0.19 -0.8
3,723,875 + trpA BNILDI_18480 0.38 -2.0
3,723,875 + trpA BNILDI_18480 0.38 -1.3
3,723,892 + trpA BNILDI_18480 0.40 +0.0
3,723,893 - trpA BNILDI_18480 0.40 -4.2
3,723,915 - trpA BNILDI_18480 0.43 -3.0
3,724,009 + trpA BNILDI_18480 0.54 -3.2
3,724,010 - trpA BNILDI_18480 0.54 -2.3
3,724,021 + trpA BNILDI_18480 0.56 -3.9
3,724,021 + trpA BNILDI_18480 0.56 -5.1
3,724,021 + trpA BNILDI_18480 0.56 -3.8
3,724,021 + trpA BNILDI_18480 0.56 -3.8
3,724,022 - trpA BNILDI_18480 0.56 -4.1
3,724,077 - trpA BNILDI_18480 0.63 -2.1
3,724,094 + trpA BNILDI_18480 0.65 -1.1
3,724,094 + trpA BNILDI_18480 0.65 -3.8
3,724,094 + trpA BNILDI_18480 0.65 -4.0
3,724,459 - +0.9

Or see this region's nucleotide sequence