Strain Fitness in Escherichia coli ECRC62 around BNILDI_18365

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciT and osmB are separated by 268 nucleotidesosmB and yciH are separated by 125 nucleotidesyciH and pyrF overlap by 1 nucleotidespyrF and lapB are separated by 132 nucleotides BNILDI_18350: yciT - DNA-binding transcriptional regulator YciT, at 3,698,484 to 3,699,233 yciT BNILDI_18355: osmB - osmotically-inducible lipoprotein OsmB, at 3,699,502 to 3,699,720 osmB BNILDI_18360: yciH - stress response translation initiation inhibitor YciH, at 3,699,846 to 3,700,172 yciH BNILDI_18365: pyrF - orotidine-5'-phosphate decarboxylase, at 3,700,172 to 3,700,969 pyrF BNILDI_18370: lapB - lipopolysaccharide assembly protein LapB, at 3,701,102 to 3,702,271 lapB Position (kb) 3700 3701Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3699.204 kb on - strandat 3699.326 kb on + strandat 3699.390 kb on + strandat 3699.390 kb on + strandat 3699.444 kb on - strandat 3699.448 kb on - strandat 3699.452 kb on + strandat 3699.467 kb on + strandat 3699.498 kb on + strandat 3699.501 kb on + strandat 3699.586 kb on - strand, within osmBat 3699.671 kb on + strand, within osmBat 3699.672 kb on - strand, within osmBat 3699.942 kb on - strand, within yciHat 3700.099 kb on - strand, within yciHat 3700.125 kb on - strand, within yciHat 3700.187 kb on + strandat 3700.260 kb on + strand, within pyrFat 3700.260 kb on + strand, within pyrFat 3700.261 kb on - strand, within pyrFat 3700.261 kb on - strand, within pyrFat 3700.298 kb on - strand, within pyrFat 3700.298 kb on - strand, within pyrFat 3700.374 kb on - strand, within pyrFat 3700.595 kb on - strand, within pyrFat 3700.595 kb on - strand, within pyrFat 3700.638 kb on - strand, within pyrFat 3700.759 kb on + strand, within pyrFat 3700.837 kb on + strand, within pyrFat 3700.838 kb on - strand, within pyrFat 3700.838 kb on - strand, within pyrFat 3700.843 kb on - strand, within pyrFat 3700.901 kb on + strandat 3700.902 kb on - strandat 3700.992 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,699,204 - +1.0
3,699,326 + -0.4
3,699,390 + +1.5
3,699,390 + +1.1
3,699,444 - +0.9
3,699,448 - -0.1
3,699,452 + -1.1
3,699,467 + -3.0
3,699,498 + -0.3
3,699,501 + -0.1
3,699,586 - osmB BNILDI_18355 0.38 +0.0
3,699,671 + osmB BNILDI_18355 0.77 -1.8
3,699,672 - osmB BNILDI_18355 0.78 +0.0
3,699,942 - yciH BNILDI_18360 0.29 +0.0
3,700,099 - yciH BNILDI_18360 0.77 -0.1
3,700,125 - yciH BNILDI_18360 0.85 -0.0
3,700,187 + -0.8
3,700,260 + pyrF BNILDI_18365 0.11 -3.6
3,700,260 + pyrF BNILDI_18365 0.11 -1.2
3,700,261 - pyrF BNILDI_18365 0.11 -4.7
3,700,261 - pyrF BNILDI_18365 0.11 -2.2
3,700,298 - pyrF BNILDI_18365 0.16 -2.5
3,700,298 - pyrF BNILDI_18365 0.16 -3.0
3,700,374 - pyrF BNILDI_18365 0.25 -5.1
3,700,595 - pyrF BNILDI_18365 0.53 -3.4
3,700,595 - pyrF BNILDI_18365 0.53 -1.6
3,700,638 - pyrF BNILDI_18365 0.58 -2.6
3,700,759 + pyrF BNILDI_18365 0.74 -3.0
3,700,837 + pyrF BNILDI_18365 0.83 -2.8
3,700,838 - pyrF BNILDI_18365 0.83 -1.5
3,700,838 - pyrF BNILDI_18365 0.83 -3.5
3,700,843 - pyrF BNILDI_18365 0.84 -1.7
3,700,901 + -1.9
3,700,902 - -2.9
3,700,992 + +0.3

Or see this region's nucleotide sequence