Strain Fitness in Escherichia coli ECRC62 around BNILDI_18135

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaG and mpaA are separated by 513 nucleotidesmpaA and ycjG overlap by 26 nucleotides BNILDI_18130: wcaG - NAD-dependent dehydratase, at 3,651,292 to 3,651,942 wcaG BNILDI_18135: mpaA - murein tripeptide amidase MpaA, at 3,652,456 to 3,652,962 mpaA BNILDI_18140: ycjG - L-Ala-D/L-Glu epimerase, at 3,652,937 to 3,653,902 ycjG Position (kb) 3652 3653Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3651.464 kb on + strand, within wcaGat 3651.464 kb on + strand, within wcaGat 3651.514 kb on + strand, within wcaGat 3651.515 kb on - strand, within wcaGat 3651.657 kb on + strand, within wcaGat 3651.732 kb on + strand, within wcaGat 3651.768 kb on + strand, within wcaGat 3651.768 kb on + strand, within wcaGat 3651.768 kb on + strand, within wcaGat 3651.771 kb on + strand, within wcaGat 3651.772 kb on - strand, within wcaGat 3651.778 kb on + strand, within wcaGat 3651.778 kb on + strand, within wcaGat 3651.787 kb on - strand, within wcaGat 3651.814 kb on - strand, within wcaGat 3651.828 kb on + strand, within wcaGat 3651.828 kb on + strand, within wcaGat 3651.829 kb on - strand, within wcaGat 3651.829 kb on - strand, within wcaGat 3651.845 kb on + strand, within wcaGat 3651.849 kb on + strand, within wcaGat 3651.856 kb on - strand, within wcaGat 3651.893 kb on + strandat 3651.974 kb on - strandat 3652.069 kb on + strandat 3652.070 kb on - strandat 3652.089 kb on - strandat 3652.091 kb on + strandat 3652.092 kb on - strandat 3652.112 kb on - strandat 3652.211 kb on - strandat 3652.240 kb on + strandat 3652.509 kb on + strand, within mpaAat 3652.509 kb on + strand, within mpaAat 3652.607 kb on + strand, within mpaAat 3652.608 kb on - strand, within mpaAat 3652.608 kb on - strand, within mpaAat 3652.610 kb on + strand, within mpaAat 3652.666 kb on - strand, within mpaAat 3652.666 kb on - strand, within mpaAat 3652.666 kb on - strand, within mpaAat 3652.719 kb on + strand, within mpaAat 3652.719 kb on + strand, within mpaAat 3652.719 kb on + strand, within mpaAat 3652.938 kb on + strandat 3652.939 kb on - strandat 3652.985 kb on + strandat 3652.986 kb on - strandat 3653.164 kb on + strand, within ycjGat 3653.167 kb on + strand, within ycjGat 3653.168 kb on - strand, within ycjGat 3653.171 kb on - strand, within ycjGat 3653.254 kb on + strand, within ycjGat 3653.327 kb on + strand, within ycjGat 3653.328 kb on - strand, within ycjGat 3653.434 kb on + strand, within ycjGat 3653.434 kb on + strand, within ycjGat 3653.638 kb on + strand, within ycjGat 3653.638 kb on + strand, within ycjGat 3653.683 kb on + strand, within ycjGat 3653.860 kb on + strandat 3653.861 kb on - strandat 3653.861 kb on - strandat 3653.864 kb on + strandat 3653.886 kb on - strandat 3653.886 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,651,464 + wcaG BNILDI_18130 0.26 +0.2
3,651,464 + wcaG BNILDI_18130 0.26 +0.3
3,651,514 + wcaG BNILDI_18130 0.34 -0.1
3,651,515 - wcaG BNILDI_18130 0.34 +2.1
3,651,657 + wcaG BNILDI_18130 0.56 +0.2
3,651,732 + wcaG BNILDI_18130 0.68 -1.1
3,651,768 + wcaG BNILDI_18130 0.73 +1.2
3,651,768 + wcaG BNILDI_18130 0.73 +0.1
3,651,768 + wcaG BNILDI_18130 0.73 -0.3
3,651,771 + wcaG BNILDI_18130 0.74 -0.7
3,651,772 - wcaG BNILDI_18130 0.74 -0.4
3,651,778 + wcaG BNILDI_18130 0.75 -0.1
3,651,778 + wcaG BNILDI_18130 0.75 -0.3
3,651,787 - wcaG BNILDI_18130 0.76 +0.3
3,651,814 - wcaG BNILDI_18130 0.80 +0.3
3,651,828 + wcaG BNILDI_18130 0.82 -1.2
3,651,828 + wcaG BNILDI_18130 0.82 +1.3
3,651,829 - wcaG BNILDI_18130 0.82 +2.2
3,651,829 - wcaG BNILDI_18130 0.82 -0.9
3,651,845 + wcaG BNILDI_18130 0.85 -0.2
3,651,849 + wcaG BNILDI_18130 0.86 +1.4
3,651,856 - wcaG BNILDI_18130 0.87 +1.2
3,651,893 + -0.1
3,651,974 - -0.7
3,652,069 + -0.6
3,652,070 - -1.9
3,652,089 - -0.6
3,652,091 + -0.3
3,652,092 - -2.9
3,652,112 - +1.0
3,652,211 - -0.1
3,652,240 + -1.2
3,652,509 + mpaA BNILDI_18135 0.10 -0.8
3,652,509 + mpaA BNILDI_18135 0.10 +0.9
3,652,607 + mpaA BNILDI_18135 0.30 -0.8
3,652,608 - mpaA BNILDI_18135 0.30 -0.9
3,652,608 - mpaA BNILDI_18135 0.30 +0.1
3,652,610 + mpaA BNILDI_18135 0.30 +0.4
3,652,666 - mpaA BNILDI_18135 0.41 -0.0
3,652,666 - mpaA BNILDI_18135 0.41 -1.4
3,652,666 - mpaA BNILDI_18135 0.41 +0.2
3,652,719 + mpaA BNILDI_18135 0.52 +0.8
3,652,719 + mpaA BNILDI_18135 0.52 +0.8
3,652,719 + mpaA BNILDI_18135 0.52 +0.2
3,652,938 + +0.1
3,652,939 - +0.4
3,652,985 + +1.1
3,652,986 - +0.2
3,653,164 + ycjG BNILDI_18140 0.23 +2.7
3,653,167 + ycjG BNILDI_18140 0.24 -0.3
3,653,168 - ycjG BNILDI_18140 0.24 +1.1
3,653,171 - ycjG BNILDI_18140 0.24 +0.1
3,653,254 + ycjG BNILDI_18140 0.33 -0.3
3,653,327 + ycjG BNILDI_18140 0.40 -0.1
3,653,328 - ycjG BNILDI_18140 0.40 +0.2
3,653,434 + ycjG BNILDI_18140 0.51 +0.4
3,653,434 + ycjG BNILDI_18140 0.51 -0.5
3,653,638 + ycjG BNILDI_18140 0.73 -1.2
3,653,638 + ycjG BNILDI_18140 0.73 +0.0
3,653,683 + ycjG BNILDI_18140 0.77 +0.7
3,653,860 + +0.1
3,653,861 - +0.3
3,653,861 - -1.7
3,653,864 + -0.7
3,653,886 - +0.1
3,653,886 - -0.3

Or see this region's nucleotide sequence