Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbD and pdxI are separated by 63 nucleotides pdxI and paaY are separated by 230 nucleotides paaY and paaX overlap by 19 nucleotides
BNILDI_17875: ydbD - Uncharacterized protein YdbD, at 3,597,026 to 3,599,332
ydbD
BNILDI_17880: pdxI - pyridoxine 4-dehydrogenase, at 3,599,396 to 3,600,256
pdxI
BNILDI_17885: paaY - phenylacetic acid degradation protein PaaY, at 3,600,487 to 3,601,077
paaY
BNILDI_17890: paaX - phenylacetic acid degradation operon negative regulatory protein PaaX, at 3,601,059 to 3,602,009
paaX
Position (kb)
3599
3600
3601 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3598.450 kb on + strand, within ydbD at 3598.868 kb on - strand, within ydbD at 3599.032 kb on + strand, within ydbD at 3599.173 kb on + strand at 3599.285 kb on - strand at 3599.332 kb on + strand at 3599.332 kb on + strand at 3599.332 kb on + strand at 3599.332 kb on + strand at 3599.332 kb on + strand at 3599.333 kb on - strand at 3599.389 kb on - strand at 3599.704 kb on - strand, within pdxI at 3599.875 kb on + strand, within pdxI at 3599.918 kb on - strand, within pdxI at 3599.922 kb on - strand, within pdxI at 3599.922 kb on - strand, within pdxI at 3599.927 kb on - strand, within pdxI at 3600.008 kb on - strand, within pdxI at 3600.082 kb on - strand, within pdxI at 3600.140 kb on + strand, within pdxI at 3600.141 kb on - strand, within pdxI at 3600.143 kb on - strand, within pdxI at 3600.245 kb on - strand at 3600.299 kb on + strand at 3600.342 kb on + strand at 3600.445 kb on - strand at 3600.465 kb on + strand at 3600.517 kb on + strand at 3600.518 kb on - strand at 3600.790 kb on + strand, within paaY at 3600.840 kb on + strand, within paaY at 3600.841 kb on - strand, within paaY at 3600.898 kb on - strand, within paaY at 3600.898 kb on - strand, within paaY at 3600.898 kb on - strand, within paaY at 3601.060 kb on + strand at 3601.060 kb on + strand at 3601.061 kb on - strand at 3601.207 kb on + strand, within paaX at 3601.208 kb on - strand, within paaX at 3601.218 kb on - strand, within paaX at 3601.236 kb on - strand, within paaX
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,598,450 + ydbD BNILDI_17875 0.62 -0.5 3,598,868 - ydbD BNILDI_17875 0.80 +0.5 3,599,032 + ydbD BNILDI_17875 0.87 -1.6 3,599,173 + -0.5 3,599,285 - +0.4 3,599,332 + -0.1 3,599,332 + +0.5 3,599,332 + -0.9 3,599,332 + -2.2 3,599,332 + -1.0 3,599,333 - -0.4 3,599,389 - -0.6 3,599,704 - pdxI BNILDI_17880 0.36 -0.5 3,599,875 + pdxI BNILDI_17880 0.56 -0.4 3,599,918 - pdxI BNILDI_17880 0.61 -0.5 3,599,922 - pdxI BNILDI_17880 0.61 +0.3 3,599,922 - pdxI BNILDI_17880 0.61 +1.1 3,599,927 - pdxI BNILDI_17880 0.62 +0.8 3,600,008 - pdxI BNILDI_17880 0.71 +1.8 3,600,082 - pdxI BNILDI_17880 0.80 +0.9 3,600,140 + pdxI BNILDI_17880 0.86 +0.3 3,600,141 - pdxI BNILDI_17880 0.87 -0.5 3,600,143 - pdxI BNILDI_17880 0.87 -0.9 3,600,245 - -0.7 3,600,299 + +0.3 3,600,342 + -0.3 3,600,445 - -0.2 3,600,465 + -0.2 3,600,517 + -1.9 3,600,518 - +0.6 3,600,790 + paaY BNILDI_17885 0.51 -0.4 3,600,840 + paaY BNILDI_17885 0.60 -0.7 3,600,841 - paaY BNILDI_17885 0.60 -0.5 3,600,898 - paaY BNILDI_17885 0.70 +0.3 3,600,898 - paaY BNILDI_17885 0.70 -0.2 3,600,898 - paaY BNILDI_17885 0.70 -0.3 3,601,060 + -1.0 3,601,060 + +0.2 3,601,061 - +0.1 3,601,207 + paaX BNILDI_17890 0.16 +1.2 3,601,208 - paaX BNILDI_17890 0.16 -0.1 3,601,218 - paaX BNILDI_17890 0.17 -0.0 3,601,236 - paaX BNILDI_17890 0.19 -0.3
Or see this region's nucleotide sequence