Strain Fitness in Escherichia coli ECRC62 around BNILDI_14645

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaK and wcaL overlap by 4 nucleotideswcaL and wcaM are separated by 10 nucleotides BNILDI_14640: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 2,956,437 to 2,957,717 wcaK BNILDI_14645: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 2,957,714 to 2,958,934 wcaL BNILDI_14650: wcaM - colanic acid biosynthesis protein WcaM, at 2,958,945 to 2,960,339 wcaM Position (kb) 2957 2958 2959Strain fitness (log2 ratio) -2 -1 0 1 2at 2956.736 kb on + strand, within wcaKat 2956.737 kb on - strand, within wcaKat 2956.833 kb on + strand, within wcaKat 2956.871 kb on + strand, within wcaKat 2956.934 kb on + strand, within wcaKat 2956.934 kb on + strand, within wcaKat 2956.943 kb on - strand, within wcaKat 2956.950 kb on + strand, within wcaKat 2957.021 kb on + strand, within wcaKat 2957.101 kb on - strand, within wcaKat 2957.298 kb on + strand, within wcaKat 2957.298 kb on + strand, within wcaKat 2957.299 kb on - strand, within wcaKat 2957.299 kb on - strand, within wcaKat 2957.299 kb on - strand, within wcaKat 2957.300 kb on + strand, within wcaKat 2957.368 kb on + strand, within wcaKat 2957.369 kb on - strand, within wcaKat 2957.369 kb on - strand, within wcaKat 2957.456 kb on + strand, within wcaKat 2957.457 kb on - strand, within wcaKat 2957.504 kb on + strand, within wcaKat 2957.505 kb on - strand, within wcaKat 2957.556 kb on - strand, within wcaKat 2957.567 kb on - strand, within wcaKat 2957.868 kb on - strand, within wcaLat 2957.908 kb on + strand, within wcaLat 2957.909 kb on - strand, within wcaLat 2957.909 kb on - strand, within wcaLat 2957.974 kb on + strand, within wcaLat 2958.158 kb on - strand, within wcaLat 2958.331 kb on - strand, within wcaLat 2958.331 kb on - strand, within wcaLat 2958.334 kb on + strand, within wcaLat 2958.536 kb on + strand, within wcaLat 2958.537 kb on - strand, within wcaLat 2958.571 kb on + strand, within wcaLat 2958.884 kb on - strandat 2958.932 kb on + strandat 2958.933 kb on - strandat 2958.953 kb on + strandat 2959.062 kb on + strandat 2959.238 kb on - strand, within wcaMat 2959.277 kb on + strand, within wcaMat 2959.277 kb on + strand, within wcaMat 2959.278 kb on - strand, within wcaMat 2959.284 kb on - strand, within wcaMat 2959.365 kb on + strand, within wcaMat 2959.366 kb on - strand, within wcaMat 2959.367 kb on + strand, within wcaMat 2959.367 kb on + strand, within wcaMat 2959.368 kb on - strand, within wcaMat 2959.438 kb on - strand, within wcaMat 2959.595 kb on + strand, within wcaMat 2959.647 kb on + strand, within wcaMat 2959.648 kb on - strand, within wcaMat 2959.923 kb on + strand, within wcaM

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,956,736 + wcaK BNILDI_14640 0.23 +0.0
2,956,737 - wcaK BNILDI_14640 0.23 -0.5
2,956,833 + wcaK BNILDI_14640 0.31 +1.2
2,956,871 + wcaK BNILDI_14640 0.34 -1.9
2,956,934 + wcaK BNILDI_14640 0.39 -0.2
2,956,934 + wcaK BNILDI_14640 0.39 -0.7
2,956,943 - wcaK BNILDI_14640 0.40 -0.2
2,956,950 + wcaK BNILDI_14640 0.40 +1.3
2,957,021 + wcaK BNILDI_14640 0.46 +0.2
2,957,101 - wcaK BNILDI_14640 0.52 -0.4
2,957,298 + wcaK BNILDI_14640 0.67 -0.0
2,957,298 + wcaK BNILDI_14640 0.67 +0.2
2,957,299 - wcaK BNILDI_14640 0.67 -1.2
2,957,299 - wcaK BNILDI_14640 0.67 +0.7
2,957,299 - wcaK BNILDI_14640 0.67 +0.1
2,957,300 + wcaK BNILDI_14640 0.67 -0.7
2,957,368 + wcaK BNILDI_14640 0.73 +0.1
2,957,369 - wcaK BNILDI_14640 0.73 -0.1
2,957,369 - wcaK BNILDI_14640 0.73 -0.2
2,957,456 + wcaK BNILDI_14640 0.80 -0.3
2,957,457 - wcaK BNILDI_14640 0.80 +0.2
2,957,504 + wcaK BNILDI_14640 0.83 +0.6
2,957,505 - wcaK BNILDI_14640 0.83 +0.2
2,957,556 - wcaK BNILDI_14640 0.87 -0.1
2,957,567 - wcaK BNILDI_14640 0.88 +1.1
2,957,868 - wcaL BNILDI_14645 0.13 -1.1
2,957,908 + wcaL BNILDI_14645 0.16 -0.3
2,957,909 - wcaL BNILDI_14645 0.16 -0.1
2,957,909 - wcaL BNILDI_14645 0.16 +0.2
2,957,974 + wcaL BNILDI_14645 0.21 -0.3
2,958,158 - wcaL BNILDI_14645 0.36 -2.2
2,958,331 - wcaL BNILDI_14645 0.51 -1.3
2,958,331 - wcaL BNILDI_14645 0.51 -0.8
2,958,334 + wcaL BNILDI_14645 0.51 -0.8
2,958,536 + wcaL BNILDI_14645 0.67 -0.0
2,958,537 - wcaL BNILDI_14645 0.67 +1.0
2,958,571 + wcaL BNILDI_14645 0.70 +0.4
2,958,884 - +0.6
2,958,932 + -1.6
2,958,933 - +0.1
2,958,953 + +2.2
2,959,062 + +1.1
2,959,238 - wcaM BNILDI_14650 0.21 -0.3
2,959,277 + wcaM BNILDI_14650 0.24 +0.3
2,959,277 + wcaM BNILDI_14650 0.24 -1.1
2,959,278 - wcaM BNILDI_14650 0.24 +0.0
2,959,284 - wcaM BNILDI_14650 0.24 +0.0
2,959,365 + wcaM BNILDI_14650 0.30 -0.1
2,959,366 - wcaM BNILDI_14650 0.30 -0.9
2,959,367 + wcaM BNILDI_14650 0.30 +0.1
2,959,367 + wcaM BNILDI_14650 0.30 +0.3
2,959,368 - wcaM BNILDI_14650 0.30 -1.2
2,959,438 - wcaM BNILDI_14650 0.35 +0.3
2,959,595 + wcaM BNILDI_14650 0.47 +0.4
2,959,647 + wcaM BNILDI_14650 0.50 +0.5
2,959,648 - wcaM BNILDI_14650 0.50 -0.1
2,959,923 + wcaM BNILDI_14650 0.70 +0.1

Or see this region's nucleotide sequence