Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk1 and purN are separated by 170 nucleotides purN and purM overlap by 1 nucleotides
BNILDI_12260: ppk1 - polyphosphate kinase 1, at 2,461,236 to 2,463,302
ppk1
BNILDI_12265: purN - phosphoribosylglycinamide formyltransferase, at 2,463,473 to 2,464,111
purN
BNILDI_12270: purM - Phosphoribosylformylglycinamidine cyclo-ligase, at 2,464,111 to 2,465,148
purM
Position (kb)
2463
2464
2465 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2462.538 kb on + strand, within ppk1 at 2462.599 kb on - strand, within ppk1 at 2462.635 kb on + strand, within ppk1 at 2462.635 kb on + strand, within ppk1 at 2462.842 kb on + strand, within ppk1 at 2462.843 kb on - strand, within ppk1 at 2462.843 kb on - strand, within ppk1 at 2462.914 kb on + strand, within ppk1 at 2463.010 kb on - strand, within ppk1 at 2463.019 kb on + strand, within ppk1 at 2463.019 kb on + strand, within ppk1 at 2463.154 kb on + strand at 2463.155 kb on - strand at 2463.178 kb on + strand at 2463.283 kb on + strand at 2463.283 kb on + strand at 2463.284 kb on - strand at 2463.286 kb on - strand at 2463.313 kb on - strand at 2463.344 kb on + strand at 2463.344 kb on + strand at 2463.347 kb on + strand at 2463.377 kb on + strand at 2463.464 kb on + strand at 2463.568 kb on + strand, within purN at 2463.712 kb on + strand, within purN at 2463.768 kb on + strand, within purN at 2463.879 kb on + strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.913 kb on - strand, within purN at 2464.042 kb on + strand, within purN at 2464.061 kb on - strand at 2464.093 kb on + strand at 2464.093 kb on + strand at 2464.094 kb on - strand at 2464.106 kb on - strand at 2464.266 kb on - strand, within purM at 2464.288 kb on - strand, within purM at 2464.357 kb on - strand, within purM at 2464.357 kb on - strand, within purM at 2464.357 kb on - strand, within purM at 2464.357 kb on - strand, within purM at 2464.359 kb on + strand, within purM at 2464.464 kb on - strand, within purM at 2464.629 kb on + strand, within purM at 2464.703 kb on + strand, within purM at 2464.703 kb on + strand, within purM at 2464.703 kb on + strand, within purM at 2464.824 kb on - strand, within purM at 2464.944 kb on + strand, within purM at 2464.948 kb on + strand, within purM at 2465.062 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,462,538 + ppk1 BNILDI_12260 0.63 -0.2 2,462,599 - ppk1 BNILDI_12260 0.66 +0.5 2,462,635 + ppk1 BNILDI_12260 0.68 -3.0 2,462,635 + ppk1 BNILDI_12260 0.68 -1.7 2,462,842 + ppk1 BNILDI_12260 0.78 -0.4 2,462,843 - ppk1 BNILDI_12260 0.78 +0.2 2,462,843 - ppk1 BNILDI_12260 0.78 +2.3 2,462,914 + ppk1 BNILDI_12260 0.81 -0.1 2,463,010 - ppk1 BNILDI_12260 0.86 -0.6 2,463,019 + ppk1 BNILDI_12260 0.86 +0.9 2,463,019 + ppk1 BNILDI_12260 0.86 +1.0 2,463,154 + -1.0 2,463,155 - -0.1 2,463,178 + +0.0 2,463,283 + +1.1 2,463,283 + -0.1 2,463,284 - -0.5 2,463,286 - +1.3 2,463,313 - -0.2 2,463,344 + +0.1 2,463,344 + +0.5 2,463,347 + +0.7 2,463,377 + +0.7 2,463,464 + -0.3 2,463,568 + purN BNILDI_12265 0.15 -1.4 2,463,712 + purN BNILDI_12265 0.37 -2.5 2,463,768 + purN BNILDI_12265 0.46 +0.3 2,463,879 + purN BNILDI_12265 0.64 -0.5 2,463,880 - purN BNILDI_12265 0.64 -1.1 2,463,880 - purN BNILDI_12265 0.64 -1.1 2,463,880 - purN BNILDI_12265 0.64 -2.0 2,463,880 - purN BNILDI_12265 0.64 -0.8 2,463,880 - purN BNILDI_12265 0.64 -0.9 2,463,913 - purN BNILDI_12265 0.69 +0.7 2,464,042 + purN BNILDI_12265 0.89 -0.6 2,464,061 - -0.8 2,464,093 + +0.6 2,464,093 + -0.0 2,464,094 - -0.6 2,464,106 - -0.4 2,464,266 - purM BNILDI_12270 0.15 -1.6 2,464,288 - purM BNILDI_12270 0.17 -4.3 2,464,357 - purM BNILDI_12270 0.24 -3.3 2,464,357 - purM BNILDI_12270 0.24 -1.8 2,464,357 - purM BNILDI_12270 0.24 -3.4 2,464,357 - purM BNILDI_12270 0.24 -4.1 2,464,359 + purM BNILDI_12270 0.24 -1.0 2,464,464 - purM BNILDI_12270 0.34 -1.6 2,464,629 + purM BNILDI_12270 0.50 -5.1 2,464,703 + purM BNILDI_12270 0.57 -3.8 2,464,703 + purM BNILDI_12270 0.57 -4.3 2,464,703 + purM BNILDI_12270 0.57 -3.2 2,464,824 - purM BNILDI_12270 0.69 -1.1 2,464,944 + purM BNILDI_12270 0.80 -3.1 2,464,948 + purM BNILDI_12270 0.81 -5.9 2,465,062 + -3.7
Or see this region's nucleotide sequence