Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iscS and iscU are separated by 27 nucleotides iscU and iscA are separated by 16 nucleotides iscA and hscB are separated by 95 nucleotides hscB and hscA are separated by 16 nucleotides
BNILDI_12090: iscS - cysteine desulfurase, at 2,423,878 to 2,425,092
iscS
BNILDI_12095: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,425,120 to 2,425,506
iscU
BNILDI_12100: iscA - iron-sulfur cluster assembly protein IscA, at 2,425,523 to 2,425,846
iscA
BNILDI_12105: hscB - co-chaperone HscB, at 2,425,942 to 2,426,457
hscB
BNILDI_12110: hscA - Fe-S protein assembly chaperone HscA, at 2,426,474 to 2,428,324
hscA
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-2
-1
0
1 at 2425.000 kb on - strand at 2425.531 kb on + strand at 2425.786 kb on + strand, within iscA at 2425.807 kb on + strand, within iscA at 2425.896 kb on + strand at 2426.033 kb on - strand, within hscB at 2426.150 kb on - strand, within hscB at 2427.397 kb on - strand, within hscA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,425,000 - -1.8 2,425,531 + -0.6 2,425,786 + iscA BNILDI_12100 0.81 -0.3 2,425,807 + iscA BNILDI_12100 0.88 +0.4 2,425,896 + -2.7 2,426,033 - hscB BNILDI_12105 0.18 -1.3 2,426,150 - hscB BNILDI_12105 0.40 -2.0 2,427,397 - hscA BNILDI_12110 0.50 -1.3
Or see this region's nucleotide sequence