Strain Fitness in Escherichia coli ECRC62 around BNILDI_12105

Experiment: D-Glucose

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntiscS and iscU are separated by 27 nucleotidesiscU and iscA are separated by 16 nucleotidesiscA and hscB are separated by 95 nucleotideshscB and hscA are separated by 16 nucleotides BNILDI_12090: iscS - cysteine desulfurase, at 2,423,878 to 2,425,092 iscS BNILDI_12095: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,425,120 to 2,425,506 iscU BNILDI_12100: iscA - iron-sulfur cluster assembly protein IscA, at 2,425,523 to 2,425,846 iscA BNILDI_12105: hscB - co-chaperone HscB, at 2,425,942 to 2,426,457 hscB BNILDI_12110: hscA - Fe-S protein assembly chaperone HscA, at 2,426,474 to 2,428,324 hscA Position (kb) 2425 2426 2427Strain fitness (log2 ratio) -2 -1 0 1at 2425.000 kb on - strandat 2425.531 kb on + strandat 2425.786 kb on + strand, within iscAat 2425.807 kb on + strand, within iscAat 2425.896 kb on + strandat 2426.033 kb on - strand, within hscBat 2426.150 kb on - strand, within hscBat 2427.397 kb on - strand, within hscA

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
remove
2,425,000 - -1.8
2,425,531 + -0.6
2,425,786 + iscA BNILDI_12100 0.81 -0.3
2,425,807 + iscA BNILDI_12100 0.88 +0.4
2,425,896 + -2.7
2,426,033 - hscB BNILDI_12105 0.18 -1.3
2,426,150 - hscB BNILDI_12105 0.40 -2.0
2,427,397 - hscA BNILDI_12110 0.50 -1.3

Or see this region's nucleotide sequence