Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_11590 and smpB are separated by 685 nucleotides smpB and ratA are separated by 131 nucleotides ratA and rnfH overlap by 11 nucleotides rnfH and bamE are separated by 61 nucleotides
BNILDI_11590: BNILDI_11590 - reverse transcriptase, at 2,328,332 to 2,329,105
_11590
BNILDI_11600: smpB - SsrA-binding protein SmpB, at 2,329,791 to 2,330,273
smpB
BNILDI_11605: ratA - type II toxin-antitoxin system toxin RatA, at 2,330,405 to 2,330,881
ratA
BNILDI_11610: rnfH - RnfH family protein, at 2,330,871 to 2,331,161
rnfH
BNILDI_11615: bamE - outer membrane protein assembly factor BamE, at 2,331,223 to 2,331,564
bamE
Position (kb)
2329
2330
2331 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2328.795 kb on + strand, within BNILDI_11590 at 2328.795 kb on + strand, within BNILDI_11590 at 2328.796 kb on - strand, within BNILDI_11590 at 2328.796 kb on - strand, within BNILDI_11590 at 2328.884 kb on - strand, within BNILDI_11590 at 2328.910 kb on + strand, within BNILDI_11590 at 2328.971 kb on - strand, within BNILDI_11590 at 2328.971 kb on - strand, within BNILDI_11590 at 2329.029 kb on - strand at 2329.044 kb on + strand at 2329.097 kb on - strand at 2329.103 kb on + strand at 2329.119 kb on + strand at 2329.119 kb on + strand at 2329.120 kb on - strand at 2329.120 kb on - strand at 2329.120 kb on - strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.123 kb on - strand at 2329.269 kb on - strand at 2329.288 kb on - strand at 2329.331 kb on - strand at 2329.331 kb on - strand at 2329.457 kb on - strand at 2329.509 kb on - strand at 2329.590 kb on - strand at 2329.648 kb on + strand at 2329.649 kb on - strand at 2329.680 kb on - strand at 2329.722 kb on + strand at 2329.723 kb on - strand at 2330.110 kb on + strand, within smpB at 2330.111 kb on - strand, within smpB at 2330.344 kb on - strand at 2330.348 kb on + strand at 2330.349 kb on - strand at 2330.503 kb on - strand, within ratA at 2330.901 kb on + strand, within rnfH at 2330.902 kb on - strand, within rnfH at 2331.013 kb on + strand, within rnfH at 2331.160 kb on - strand at 2331.160 kb on - strand at 2331.197 kb on + strand at 2331.224 kb on + strand at 2331.225 kb on - strand at 2331.225 kb on - strand at 2331.225 kb on - strand at 2331.225 kb on - strand at 2331.225 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,328,795 + BNILDI_11590 0.60 -0.4 2,328,795 + BNILDI_11590 0.60 +0.7 2,328,796 - BNILDI_11590 0.60 -0.4 2,328,796 - BNILDI_11590 0.60 +0.1 2,328,884 - BNILDI_11590 0.71 -0.1 2,328,910 + BNILDI_11590 0.75 +0.4 2,328,971 - BNILDI_11590 0.83 -0.8 2,328,971 - BNILDI_11590 0.83 -0.5 2,329,029 - -1.1 2,329,044 + -0.2 2,329,097 - +0.0 2,329,103 + +0.8 2,329,119 + -0.5 2,329,119 + +0.7 2,329,120 - -2.1 2,329,120 - +0.4 2,329,120 - -1.5 2,329,122 + +1.8 2,329,122 + +0.8 2,329,122 + -1.1 2,329,122 + -1.1 2,329,123 - -0.3 2,329,269 - -2.2 2,329,288 - -0.5 2,329,331 - +0.9 2,329,331 - -0.4 2,329,457 - +0.4 2,329,509 - -1.7 2,329,590 - +0.4 2,329,648 + +0.5 2,329,649 - -0.1 2,329,680 - +0.6 2,329,722 + +2.0 2,329,723 - -0.9 2,330,110 + smpB BNILDI_11600 0.66 -1.1 2,330,111 - smpB BNILDI_11600 0.66 -0.7 2,330,344 - -1.6 2,330,348 + +0.1 2,330,349 - +0.2 2,330,503 - ratA BNILDI_11605 0.21 +1.0 2,330,901 + rnfH BNILDI_11610 0.10 -0.0 2,330,902 - rnfH BNILDI_11610 0.11 -0.8 2,331,013 + rnfH BNILDI_11610 0.49 -0.9 2,331,160 - -0.2 2,331,160 - +0.7 2,331,197 + -0.2 2,331,224 + +1.0 2,331,225 - -0.0 2,331,225 - -0.6 2,331,225 - +0.1 2,331,225 - +0.8 2,331,225 - +0.8
Or see this region's nucleotide sequence