Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycI and BNILDI_11010 are separated by 24 nucleotides BNILDI_11010 and higA are separated by 70 nucleotides higA and ascB are separated by 113 nucleotides
BNILDI_11005: hycI - hydrogenase maturation peptidase HycI, at 2,223,106 to 2,223,576
hycI
BNILDI_11010: BNILDI_11010 - Periplasmic or exported protein, at 2,223,601 to 2,224,374
_11010
BNILDI_11015: higA - transcriptional regulator, at 2,224,445 to 2,224,864
higA
BNILDI_11020: ascB - 6-phospho-beta-glucosidase AscB, at 2,224,978 to 2,226,402
ascB
Position (kb)
2224
2225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2223.466 kb on + strand, within hycI at 2223.466 kb on + strand, within hycI at 2223.743 kb on - strand, within BNILDI_11010 at 2223.913 kb on + strand, within BNILDI_11010 at 2223.991 kb on + strand, within BNILDI_11010 at 2224.001 kb on + strand, within BNILDI_11010 at 2224.060 kb on + strand, within BNILDI_11010 at 2224.061 kb on - strand, within BNILDI_11010 at 2224.221 kb on - strand, within BNILDI_11010 at 2224.221 kb on - strand, within BNILDI_11010 at 2224.221 kb on - strand, within BNILDI_11010 at 2224.245 kb on + strand, within BNILDI_11010 at 2224.245 kb on + strand, within BNILDI_11010 at 2224.246 kb on - strand, within BNILDI_11010 at 2224.249 kb on + strand, within BNILDI_11010 at 2224.252 kb on - strand, within BNILDI_11010 at 2224.252 kb on - strand, within BNILDI_11010 at 2224.391 kb on + strand at 2224.403 kb on + strand at 2224.471 kb on + strand at 2224.472 kb on - strand at 2224.594 kb on + strand, within higA at 2224.594 kb on + strand, within higA at 2224.625 kb on + strand, within higA at 2224.626 kb on - strand, within higA at 2224.626 kb on - strand, within higA at 2224.721 kb on - strand, within higA at 2224.759 kb on + strand, within higA at 2224.917 kb on + strand at 2224.917 kb on + strand at 2224.934 kb on - strand at 2224.934 kb on - strand at 2224.934 kb on - strand at 2224.934 kb on - strand at 2224.934 kb on - strand at 2224.934 kb on - strand at 2224.938 kb on - strand at 2224.975 kb on + strand at 2224.976 kb on - strand at 2224.979 kb on + strand at 2224.980 kb on - strand at 2224.980 kb on - strand at 2225.009 kb on - strand at 2225.017 kb on - strand at 2225.081 kb on - strand at 2225.081 kb on - strand at 2225.085 kb on - strand at 2225.156 kb on + strand, within ascB at 2225.156 kb on + strand, within ascB at 2225.205 kb on + strand, within ascB at 2225.322 kb on - strand, within ascB at 2225.328 kb on + strand, within ascB at 2225.328 kb on + strand, within ascB at 2225.328 kb on + strand, within ascB at 2225.328 kb on + strand, within ascB at 2225.329 kb on - strand, within ascB at 2225.329 kb on - strand, within ascB at 2225.387 kb on - strand, within ascB at 2225.391 kb on - strand, within ascB at 2225.391 kb on - strand, within ascB at 2225.474 kb on + strand, within ascB at 2225.474 kb on + strand, within ascB at 2225.475 kb on - strand, within ascB at 2225.475 kb on - strand, within ascB at 2225.475 kb on - strand, within ascB at 2225.561 kb on - strand, within ascB at 2225.669 kb on + strand, within ascB at 2225.675 kb on + strand, within ascB at 2225.698 kb on - strand, within ascB at 2225.784 kb on - strand, within ascB at 2225.854 kb on - strand, within ascB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,223,466 + hycI BNILDI_11005 0.76 +0.5 2,223,466 + hycI BNILDI_11005 0.76 +0.1 2,223,743 - BNILDI_11010 0.18 +0.0 2,223,913 + BNILDI_11010 0.40 +0.1 2,223,991 + BNILDI_11010 0.50 -1.1 2,224,001 + BNILDI_11010 0.52 +0.5 2,224,060 + BNILDI_11010 0.59 -0.9 2,224,061 - BNILDI_11010 0.59 -0.8 2,224,221 - BNILDI_11010 0.80 -0.6 2,224,221 - BNILDI_11010 0.80 +0.5 2,224,221 - BNILDI_11010 0.80 -0.5 2,224,245 + BNILDI_11010 0.83 -1.3 2,224,245 + BNILDI_11010 0.83 +0.2 2,224,246 - BNILDI_11010 0.83 +0.3 2,224,249 + BNILDI_11010 0.84 -0.1 2,224,252 - BNILDI_11010 0.84 -0.4 2,224,252 - BNILDI_11010 0.84 -0.0 2,224,391 + +0.4 2,224,403 + +0.4 2,224,471 + -0.1 2,224,472 - +0.7 2,224,594 + higA BNILDI_11015 0.35 -0.9 2,224,594 + higA BNILDI_11015 0.35 -0.8 2,224,625 + higA BNILDI_11015 0.43 +0.2 2,224,626 - higA BNILDI_11015 0.43 +0.5 2,224,626 - higA BNILDI_11015 0.43 +1.1 2,224,721 - higA BNILDI_11015 0.66 +0.3 2,224,759 + higA BNILDI_11015 0.75 +0.5 2,224,917 + -0.6 2,224,917 + -1.0 2,224,934 - -0.2 2,224,934 - +0.5 2,224,934 - +0.1 2,224,934 - +0.2 2,224,934 - -0.8 2,224,934 - +0.8 2,224,938 - -2.8 2,224,975 + +1.9 2,224,976 - +0.6 2,224,979 + -0.4 2,224,980 - -0.9 2,224,980 - -0.1 2,225,009 - -1.5 2,225,017 - +0.2 2,225,081 - -0.2 2,225,081 - -0.7 2,225,085 - +0.2 2,225,156 + ascB BNILDI_11020 0.12 +0.4 2,225,156 + ascB BNILDI_11020 0.12 +0.6 2,225,205 + ascB BNILDI_11020 0.16 -0.1 2,225,322 - ascB BNILDI_11020 0.24 +0.1 2,225,328 + ascB BNILDI_11020 0.25 -0.3 2,225,328 + ascB BNILDI_11020 0.25 -1.6 2,225,328 + ascB BNILDI_11020 0.25 +0.1 2,225,328 + ascB BNILDI_11020 0.25 +0.3 2,225,329 - ascB BNILDI_11020 0.25 +0.3 2,225,329 - ascB BNILDI_11020 0.25 +0.9 2,225,387 - ascB BNILDI_11020 0.29 -0.2 2,225,391 - ascB BNILDI_11020 0.29 +0.5 2,225,391 - ascB BNILDI_11020 0.29 +0.4 2,225,474 + ascB BNILDI_11020 0.35 -0.1 2,225,474 + ascB BNILDI_11020 0.35 +0.7 2,225,475 - ascB BNILDI_11020 0.35 +0.0 2,225,475 - ascB BNILDI_11020 0.35 -0.6 2,225,475 - ascB BNILDI_11020 0.35 -0.1 2,225,561 - ascB BNILDI_11020 0.41 -0.9 2,225,669 + ascB BNILDI_11020 0.48 +0.0 2,225,675 + ascB BNILDI_11020 0.49 -0.6 2,225,698 - ascB BNILDI_11020 0.51 -1.2 2,225,784 - ascB BNILDI_11020 0.57 +0.9 2,225,854 - ascB BNILDI_11020 0.61 +0.1
Or see this region's nucleotide sequence