Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiI and yqfI are separated by 73 nucleotides yqfI and gcvT are separated by 290 nucleotides gcvT and gcvH are separated by 23 nucleotides gcvH and gcvP are separated by 118 nucleotides
BNILDI_09960: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 1,999,530 to 2,000,732
ubiI
BNILDI_09965: yqfI - Protein YqfI, at 2,000,806 to 2,000,889
yqfI
BNILDI_09970: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 2,001,180 to 2,002,274
gcvT
BNILDI_09975: gcvH - glycine cleavage system protein GcvH, at 2,002,298 to 2,002,687
gcvH
BNILDI_09980: gcvP - aminomethyl-transferring glycine dehydrogenase, at 2,002,806 to 2,005,679
gcvP
Position (kb)
2001
2002
2003 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2000.305 kb on + strand, within ubiI at 2000.306 kb on - strand, within ubiI at 2000.514 kb on + strand, within ubiI at 2000.520 kb on + strand, within ubiI at 2000.576 kb on + strand, within ubiI at 2000.577 kb on - strand, within ubiI at 2000.704 kb on + strand at 2000.705 kb on - strand at 2000.922 kb on + strand at 2000.945 kb on + strand at 2000.945 kb on + strand at 2000.946 kb on - strand at 2000.946 kb on - strand at 2000.950 kb on - strand at 2001.014 kb on + strand at 2001.015 kb on - strand at 2001.033 kb on - strand at 2001.131 kb on + strand at 2001.131 kb on + strand at 2001.267 kb on + strand at 2001.332 kb on + strand, within gcvT at 2001.332 kb on + strand, within gcvT at 2001.332 kb on + strand, within gcvT at 2001.332 kb on + strand, within gcvT at 2001.333 kb on - strand, within gcvT at 2001.333 kb on - strand, within gcvT at 2001.333 kb on - strand, within gcvT at 2001.466 kb on + strand, within gcvT at 2001.466 kb on + strand, within gcvT at 2001.467 kb on - strand, within gcvT at 2001.467 kb on - strand, within gcvT at 2001.467 kb on - strand, within gcvT at 2001.487 kb on - strand, within gcvT at 2001.487 kb on - strand, within gcvT at 2001.491 kb on + strand, within gcvT at 2001.659 kb on + strand, within gcvT at 2001.729 kb on - strand, within gcvT at 2001.747 kb on - strand, within gcvT at 2001.908 kb on + strand, within gcvT at 2001.909 kb on - strand, within gcvT at 2002.058 kb on + strand, within gcvT at 2002.125 kb on - strand, within gcvT at 2002.178 kb on + strand at 2002.308 kb on + strand at 2002.326 kb on - strand at 2002.352 kb on - strand, within gcvH at 2002.370 kb on + strand, within gcvH at 2002.371 kb on - strand, within gcvH at 2002.381 kb on + strand, within gcvH at 2002.382 kb on - strand, within gcvH at 2002.488 kb on + strand, within gcvH at 2002.508 kb on + strand, within gcvH at 2002.519 kb on - strand, within gcvH at 2002.661 kb on + strand at 2002.662 kb on - strand at 2002.675 kb on - strand at 2002.675 kb on - strand at 2002.675 kb on - strand at 2002.675 kb on - strand at 2003.056 kb on - strand at 2003.056 kb on - strand at 2003.130 kb on + strand, within gcvP at 2003.139 kb on + strand, within gcvP
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,000,305 + ubiI BNILDI_09960 0.64 -0.5 2,000,306 - ubiI BNILDI_09960 0.65 +1.3 2,000,514 + ubiI BNILDI_09960 0.82 -0.0 2,000,520 + ubiI BNILDI_09960 0.82 -0.6 2,000,576 + ubiI BNILDI_09960 0.87 +2.1 2,000,577 - ubiI BNILDI_09960 0.87 -0.5 2,000,704 + +1.2 2,000,705 - -2.6 2,000,922 + -0.5 2,000,945 + +0.1 2,000,945 + -0.4 2,000,946 - +0.4 2,000,946 - +0.1 2,000,950 - -0.1 2,001,014 + -0.3 2,001,015 - +0.2 2,001,033 - -1.0 2,001,131 + +0.5 2,001,131 + +0.5 2,001,267 + -0.2 2,001,332 + gcvT BNILDI_09970 0.14 +2.1 2,001,332 + gcvT BNILDI_09970 0.14 -1.1 2,001,332 + gcvT BNILDI_09970 0.14 -0.4 2,001,332 + gcvT BNILDI_09970 0.14 +0.3 2,001,333 - gcvT BNILDI_09970 0.14 +0.8 2,001,333 - gcvT BNILDI_09970 0.14 +0.5 2,001,333 - gcvT BNILDI_09970 0.14 -0.1 2,001,466 + gcvT BNILDI_09970 0.26 +0.6 2,001,466 + gcvT BNILDI_09970 0.26 -2.3 2,001,467 - gcvT BNILDI_09970 0.26 -0.8 2,001,467 - gcvT BNILDI_09970 0.26 -0.4 2,001,467 - gcvT BNILDI_09970 0.26 -0.2 2,001,487 - gcvT BNILDI_09970 0.28 +0.3 2,001,487 - gcvT BNILDI_09970 0.28 +0.5 2,001,491 + gcvT BNILDI_09970 0.28 +0.6 2,001,659 + gcvT BNILDI_09970 0.44 -2.3 2,001,729 - gcvT BNILDI_09970 0.50 -0.2 2,001,747 - gcvT BNILDI_09970 0.52 -0.1 2,001,908 + gcvT BNILDI_09970 0.66 +0.7 2,001,909 - gcvT BNILDI_09970 0.67 +0.0 2,002,058 + gcvT BNILDI_09970 0.80 +0.4 2,002,125 - gcvT BNILDI_09970 0.86 -0.9 2,002,178 + +0.2 2,002,308 + +0.6 2,002,326 - -0.0 2,002,352 - gcvH BNILDI_09975 0.14 +0.5 2,002,370 + gcvH BNILDI_09975 0.18 +0.3 2,002,371 - gcvH BNILDI_09975 0.19 -0.6 2,002,381 + gcvH BNILDI_09975 0.21 -0.8 2,002,382 - gcvH BNILDI_09975 0.22 -0.0 2,002,488 + gcvH BNILDI_09975 0.49 -0.5 2,002,508 + gcvH BNILDI_09975 0.54 +0.1 2,002,519 - gcvH BNILDI_09975 0.57 +1.0 2,002,661 + +3.1 2,002,662 - -0.1 2,002,675 - +0.5 2,002,675 - +1.8 2,002,675 - +0.3 2,002,675 - +1.5 2,003,056 - +0.5 2,003,056 - +3.3 2,003,130 + gcvP BNILDI_09980 0.11 -0.4 2,003,139 + gcvP BNILDI_09980 0.12 -2.9
Or see this region's nucleotide sequence