Strain Fitness in Escherichia coli ECRC62 around BNILDI_08990

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdksA and sfsA are separated by 177 nucleotidessfsA and thpR are separated by 14 nucleotidesthpR and hrpB are separated by 73 nucleotides BNILDI_08980: dksA - RNA polymerase-binding protein DksA, at 1,798,622 to 1,799,077 dksA BNILDI_08985: sfsA - DNA/RNA nuclease SfsA, at 1,799,255 to 1,799,959 sfsA BNILDI_08990: thpR - RNA 2',3'-cyclic phosphodiesterase, at 1,799,974 to 1,800,504 thpR BNILDI_08995: hrpB - ATP-dependent helicase HrpB, at 1,800,578 to 1,803,007 hrpB Position (kb) 1799 1800 1801Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1798.986 kb on + strand, within dksAat 1798.987 kb on - strand, within dksAat 1799.139 kb on - strandat 1799.143 kb on - strandat 1799.143 kb on - strandat 1799.236 kb on + strandat 1799.245 kb on - strandat 1799.248 kb on + strandat 1799.249 kb on - strandat 1799.253 kb on + strandat 1799.253 kb on + strandat 1799.256 kb on + strandat 1799.317 kb on - strandat 1799.367 kb on + strand, within sfsAat 1799.517 kb on + strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.562 kb on + strand, within sfsAat 1799.594 kb on - strand, within sfsAat 1799.594 kb on - strand, within sfsAat 1799.622 kb on + strand, within sfsAat 1799.647 kb on + strand, within sfsAat 1799.647 kb on + strand, within sfsAat 1799.649 kb on + strand, within sfsAat 1799.772 kb on + strand, within sfsAat 1799.773 kb on - strand, within sfsAat 1799.862 kb on - strand, within sfsAat 1799.862 kb on - strand, within sfsAat 1799.913 kb on + strandat 1799.925 kb on - strandat 1799.940 kb on - strandat 1800.009 kb on - strandat 1800.126 kb on + strand, within thpRat 1800.126 kb on + strand, within thpRat 1800.126 kb on + strand, within thpRat 1800.127 kb on - strand, within thpRat 1800.129 kb on + strand, within thpRat 1800.129 kb on + strand, within thpRat 1800.130 kb on - strand, within thpRat 1800.316 kb on + strand, within thpRat 1800.384 kb on + strand, within thpRat 1800.537 kb on + strandat 1800.537 kb on + strandat 1800.538 kb on - strandat 1800.615 kb on + strandat 1800.615 kb on + strandat 1800.620 kb on - strandat 1800.620 kb on - strandat 1800.648 kb on + strandat 1800.648 kb on + strandat 1800.651 kb on + strandat 1800.651 kb on + strandat 1800.651 kb on + strandat 1800.652 kb on - strandat 1800.652 kb on - strandat 1800.652 kb on - strandat 1800.718 kb on + strandat 1800.730 kb on + strandat 1800.794 kb on - strandat 1801.027 kb on + strand, within hrpBat 1801.027 kb on + strand, within hrpBat 1801.039 kb on + strand, within hrpBat 1801.039 kb on + strand, within hrpBat 1801.141 kb on - strand, within hrpBat 1801.221 kb on + strand, within hrpBat 1801.222 kb on - strand, within hrpBat 1801.222 kb on - strand, within hrpBat 1801.296 kb on + strand, within hrpBat 1801.296 kb on + strand, within hrpBat 1801.297 kb on - strand, within hrpBat 1801.297 kb on - strand, within hrpBat 1801.297 kb on - strand, within hrpBat 1801.297 kb on - strand, within hrpBat 1801.297 kb on - strand, within hrpBat 1801.314 kb on - strand, within hrpBat 1801.396 kb on + strand, within hrpB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,798,986 + dksA BNILDI_08980 0.80 -5.6
1,798,987 - dksA BNILDI_08980 0.80 -3.0
1,799,139 - +0.1
1,799,143 - -1.5
1,799,143 - -0.5
1,799,236 + -0.8
1,799,245 - +0.3
1,799,248 + +0.6
1,799,249 - +2.2
1,799,253 + +0.0
1,799,253 + -0.6
1,799,256 + +0.0
1,799,317 - +0.5
1,799,367 + sfsA BNILDI_08985 0.16 -0.5
1,799,517 + sfsA BNILDI_08985 0.37 -0.2
1,799,561 - sfsA BNILDI_08985 0.43 +0.7
1,799,561 - sfsA BNILDI_08985 0.43 -0.8
1,799,562 + sfsA BNILDI_08985 0.44 -0.1
1,799,594 - sfsA BNILDI_08985 0.48 +0.0
1,799,594 - sfsA BNILDI_08985 0.48 +0.0
1,799,622 + sfsA BNILDI_08985 0.52 +0.4
1,799,647 + sfsA BNILDI_08985 0.56 -0.7
1,799,647 + sfsA BNILDI_08985 0.56 +0.2
1,799,649 + sfsA BNILDI_08985 0.56 -1.6
1,799,772 + sfsA BNILDI_08985 0.73 -0.3
1,799,773 - sfsA BNILDI_08985 0.73 +0.5
1,799,862 - sfsA BNILDI_08985 0.86 -0.4
1,799,862 - sfsA BNILDI_08985 0.86 +0.4
1,799,913 + -1.9
1,799,925 - +1.1
1,799,940 - -1.8
1,800,009 - +2.0
1,800,126 + thpR BNILDI_08990 0.29 +1.0
1,800,126 + thpR BNILDI_08990 0.29 -2.0
1,800,126 + thpR BNILDI_08990 0.29 +0.4
1,800,127 - thpR BNILDI_08990 0.29 -1.9
1,800,129 + thpR BNILDI_08990 0.29 +0.1
1,800,129 + thpR BNILDI_08990 0.29 +0.4
1,800,130 - thpR BNILDI_08990 0.29 -0.1
1,800,316 + thpR BNILDI_08990 0.64 -2.1
1,800,384 + thpR BNILDI_08990 0.77 +0.2
1,800,537 + +0.1
1,800,537 + -0.3
1,800,538 - -0.3
1,800,615 + -2.1
1,800,615 + -0.9
1,800,620 - -0.1
1,800,620 - +0.1
1,800,648 + -0.1
1,800,648 + +0.8
1,800,651 + +0.2
1,800,651 + -0.0
1,800,651 + -0.4
1,800,652 - -0.8
1,800,652 - +0.2
1,800,652 - -1.0
1,800,718 + -0.3
1,800,730 + -0.1
1,800,794 - -0.2
1,801,027 + hrpB BNILDI_08995 0.18 +0.2
1,801,027 + hrpB BNILDI_08995 0.18 +0.6
1,801,039 + hrpB BNILDI_08995 0.19 -0.0
1,801,039 + hrpB BNILDI_08995 0.19 -2.3
1,801,141 - hrpB BNILDI_08995 0.23 -0.1
1,801,221 + hrpB BNILDI_08995 0.26 +0.4
1,801,222 - hrpB BNILDI_08995 0.27 +0.6
1,801,222 - hrpB BNILDI_08995 0.27 +0.4
1,801,296 + hrpB BNILDI_08995 0.30 +0.8
1,801,296 + hrpB BNILDI_08995 0.30 -0.9
1,801,297 - hrpB BNILDI_08995 0.30 +0.1
1,801,297 - hrpB BNILDI_08995 0.30 -0.3
1,801,297 - hrpB BNILDI_08995 0.30 -1.1
1,801,297 - hrpB BNILDI_08995 0.30 +1.5
1,801,297 - hrpB BNILDI_08995 0.30 +0.1
1,801,314 - hrpB BNILDI_08995 0.30 +0.9
1,801,396 + hrpB BNILDI_08995 0.34 +0.2

Or see this region's nucleotide sequence