Strain Fitness in Escherichia coli ECRC62 around BNILDI_08165

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotides BNILDI_08160: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 1,627,791 to 1,630,253 thrA BNILDI_08165: thrB - homoserine kinase, at 1,630,255 to 1,631,187 thrB BNILDI_08170: thrC - threonine synthase, at 1,631,188 to 1,632,474 thrC Position (kb) 1630 1631 1632Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1629.270 kb on - strand, within thrAat 1629.283 kb on + strand, within thrAat 1629.284 kb on - strand, within thrAat 1629.284 kb on - strand, within thrAat 1629.421 kb on - strand, within thrAat 1629.481 kb on - strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.553 kb on - strand, within thrAat 1629.555 kb on + strand, within thrAat 1629.556 kb on - strand, within thrAat 1629.715 kb on - strand, within thrAat 1629.715 kb on - strand, within thrAat 1629.716 kb on + strand, within thrAat 1629.716 kb on + strand, within thrAat 1629.717 kb on - strand, within thrAat 1629.717 kb on - strand, within thrAat 1629.730 kb on + strand, within thrAat 1630.035 kb on - strandat 1630.082 kb on + strandat 1630.131 kb on + strandat 1630.132 kb on - strandat 1630.141 kb on - strandat 1630.143 kb on + strandat 1630.193 kb on + strandat 1630.222 kb on + strandat 1630.222 kb on + strandat 1630.223 kb on - strandat 1630.226 kb on + strandat 1630.226 kb on + strandat 1630.244 kb on - strandat 1630.260 kb on + strandat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.443 kb on + strand, within thrBat 1630.444 kb on - strand, within thrBat 1630.522 kb on - strand, within thrBat 1630.542 kb on - strand, within thrBat 1630.821 kb on + strand, within thrBat 1630.897 kb on + strand, within thrBat 1630.941 kb on + strand, within thrBat 1630.954 kb on + strand, within thrBat 1631.061 kb on - strand, within thrBat 1631.114 kb on - strandat 1631.143 kb on - strandat 1631.197 kb on + strandat 1631.624 kb on - strand, within thrCat 1631.701 kb on - strand, within thrCat 1631.820 kb on + strand, within thrCat 1631.830 kb on - strand, within thrC

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,629,270 - thrA BNILDI_08160 0.60 -4.1
1,629,283 + thrA BNILDI_08160 0.61 -1.5
1,629,284 - thrA BNILDI_08160 0.61 -2.4
1,629,284 - thrA BNILDI_08160 0.61 -3.2
1,629,421 - thrA BNILDI_08160 0.66 -0.7
1,629,481 - thrA BNILDI_08160 0.69 -3.5
1,629,552 + thrA BNILDI_08160 0.71 -1.8
1,629,552 + thrA BNILDI_08160 0.71 -2.4
1,629,552 + thrA BNILDI_08160 0.71 -2.8
1,629,553 - thrA BNILDI_08160 0.72 -4.1
1,629,555 + thrA BNILDI_08160 0.72 -3.8
1,629,556 - thrA BNILDI_08160 0.72 -2.3
1,629,715 - thrA BNILDI_08160 0.78 -2.5
1,629,715 - thrA BNILDI_08160 0.78 -1.9
1,629,716 + thrA BNILDI_08160 0.78 -0.4
1,629,716 + thrA BNILDI_08160 0.78 -3.8
1,629,717 - thrA BNILDI_08160 0.78 -4.1
1,629,717 - thrA BNILDI_08160 0.78 -4.3
1,629,730 + thrA BNILDI_08160 0.79 -3.7
1,630,035 - -3.3
1,630,082 + -2.0
1,630,131 + -2.0
1,630,132 - -0.3
1,630,141 - -2.2
1,630,143 + -2.9
1,630,193 + -1.3
1,630,222 + -1.0
1,630,222 + -2.4
1,630,223 - -2.5
1,630,226 + -1.2
1,630,226 + -2.2
1,630,244 - -1.6
1,630,260 + -2.8
1,630,358 + thrB BNILDI_08165 0.11 -1.1
1,630,358 + thrB BNILDI_08165 0.11 -4.8
1,630,358 + thrB BNILDI_08165 0.11 -2.3
1,630,358 + thrB BNILDI_08165 0.11 -2.0
1,630,359 - thrB BNILDI_08165 0.11 -4.2
1,630,359 - thrB BNILDI_08165 0.11 -2.7
1,630,359 - thrB BNILDI_08165 0.11 -3.4
1,630,443 + thrB BNILDI_08165 0.20 -3.3
1,630,444 - thrB BNILDI_08165 0.20 -2.7
1,630,522 - thrB BNILDI_08165 0.29 -4.2
1,630,542 - thrB BNILDI_08165 0.31 -4.0
1,630,821 + thrB BNILDI_08165 0.61 -1.0
1,630,897 + thrB BNILDI_08165 0.69 -2.3
1,630,941 + thrB BNILDI_08165 0.74 -3.9
1,630,954 + thrB BNILDI_08165 0.75 -2.8
1,631,061 - thrB BNILDI_08165 0.86 -2.8
1,631,114 - -3.4
1,631,143 - -2.5
1,631,197 + -3.8
1,631,624 - thrC BNILDI_08170 0.34 -3.2
1,631,701 - thrC BNILDI_08170 0.40 -4.2
1,631,820 + thrC BNILDI_08170 0.49 -4.7
1,631,830 - thrC BNILDI_08170 0.50 -2.3

Or see this region's nucleotide sequence