Strain Fitness in Escherichia coli ECRC62 around BNILDI_06465

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfA and nrfB are separated by 44 nucleotidesnrfB and nrfC overlap by 4 nucleotidesnrfC and nrfD overlap by 4 nucleotides BNILDI_06455: nrfA - ammonia-forming nitrite reductase cytochrome c552 subunit, at 1,289,465 to 1,290,901 nrfA BNILDI_06460: nrfB - cytochrome c nitrite reductase pentaheme subunit, at 1,290,946 to 1,291,512 nrfB BNILDI_06465: nrfC - cytochrome c nitrite reductase Fe-S protein, at 1,291,509 to 1,292,180 nrfC BNILDI_06470: nrfD - cytochrome c nitrite reductase subunit NrfD, at 1,292,177 to 1,293,133 nrfD Position (kb) 1291 1292 1293Strain fitness (log2 ratio) -1 0 1 2at 1290.637 kb on + strand, within nrfAat 1290.638 kb on - strand, within nrfAat 1290.877 kb on + strandat 1290.878 kb on - strandat 1290.945 kb on + strandat 1290.945 kb on + strandat 1290.946 kb on - strandat 1290.946 kb on - strandat 1290.953 kb on + strandat 1290.953 kb on + strandat 1290.954 kb on - strandat 1290.969 kb on - strandat 1290.969 kb on - strandat 1291.105 kb on - strand, within nrfBat 1291.122 kb on + strand, within nrfBat 1291.122 kb on + strand, within nrfBat 1291.122 kb on + strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.123 kb on - strand, within nrfBat 1291.158 kb on + strand, within nrfBat 1291.255 kb on + strand, within nrfBat 1291.255 kb on + strand, within nrfBat 1291.256 kb on - strand, within nrfBat 1291.284 kb on + strand, within nrfBat 1291.285 kb on - strand, within nrfBat 1291.407 kb on - strand, within nrfBat 1291.665 kb on - strand, within nrfCat 1291.761 kb on + strand, within nrfCat 1291.775 kb on + strand, within nrfCat 1291.869 kb on - strand, within nrfCat 1292.099 kb on + strand, within nrfCat 1292.099 kb on + strand, within nrfCat 1292.099 kb on + strand, within nrfCat 1292.100 kb on - strand, within nrfCat 1292.100 kb on - strand, within nrfCat 1292.156 kb on + strandat 1292.438 kb on + strand, within nrfDat 1292.486 kb on + strand, within nrfDat 1292.487 kb on - strand, within nrfDat 1292.661 kb on - strand, within nrfDat 1292.661 kb on - strand, within nrfDat 1292.661 kb on - strand, within nrfDat 1292.937 kb on + strand, within nrfDat 1292.937 kb on + strand, within nrfDat 1292.937 kb on + strand, within nrfDat 1292.937 kb on + strand, within nrfDat 1292.938 kb on - strand, within nrfDat 1293.131 kb on + strandat 1293.160 kb on + strandat 1293.160 kb on + strandat 1293.161 kb on - strandat 1293.161 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,290,637 + nrfA BNILDI_06455 0.82 +0.7
1,290,638 - nrfA BNILDI_06455 0.82 +0.6
1,290,877 + +0.8
1,290,878 - +1.2
1,290,945 + +1.4
1,290,945 + +1.1
1,290,946 - -0.3
1,290,946 - -0.6
1,290,953 + +0.8
1,290,953 + -0.8
1,290,954 - +0.0
1,290,969 - -1.1
1,290,969 - -0.5
1,291,105 - nrfB BNILDI_06460 0.28 +0.3
1,291,122 + nrfB BNILDI_06460 0.31 -0.2
1,291,122 + nrfB BNILDI_06460 0.31 +0.4
1,291,122 + nrfB BNILDI_06460 0.31 -0.4
1,291,123 - nrfB BNILDI_06460 0.31 +1.8
1,291,123 - nrfB BNILDI_06460 0.31 -0.6
1,291,123 - nrfB BNILDI_06460 0.31 +0.1
1,291,123 - nrfB BNILDI_06460 0.31 -0.8
1,291,123 - nrfB BNILDI_06460 0.31 +0.7
1,291,123 - nrfB BNILDI_06460 0.31 +0.5
1,291,158 + nrfB BNILDI_06460 0.37 -0.4
1,291,255 + nrfB BNILDI_06460 0.54 +1.4
1,291,255 + nrfB BNILDI_06460 0.54 -0.2
1,291,256 - nrfB BNILDI_06460 0.55 -0.1
1,291,284 + nrfB BNILDI_06460 0.60 -0.6
1,291,285 - nrfB BNILDI_06460 0.60 -0.5
1,291,407 - nrfB BNILDI_06460 0.81 +0.7
1,291,665 - nrfC BNILDI_06465 0.23 +0.2
1,291,761 + nrfC BNILDI_06465 0.38 -1.0
1,291,775 + nrfC BNILDI_06465 0.40 -0.1
1,291,869 - nrfC BNILDI_06465 0.54 -0.1
1,292,099 + nrfC BNILDI_06465 0.88 +0.7
1,292,099 + nrfC BNILDI_06465 0.88 -1.0
1,292,099 + nrfC BNILDI_06465 0.88 +0.1
1,292,100 - nrfC BNILDI_06465 0.88 -1.0
1,292,100 - nrfC BNILDI_06465 0.88 +0.0
1,292,156 + +0.1
1,292,438 + nrfD BNILDI_06470 0.27 +0.5
1,292,486 + nrfD BNILDI_06470 0.32 -0.5
1,292,487 - nrfD BNILDI_06470 0.32 -0.2
1,292,661 - nrfD BNILDI_06470 0.51 +0.5
1,292,661 - nrfD BNILDI_06470 0.51 +0.1
1,292,661 - nrfD BNILDI_06470 0.51 -0.1
1,292,937 + nrfD BNILDI_06470 0.79 +0.3
1,292,937 + nrfD BNILDI_06470 0.79 +0.1
1,292,937 + nrfD BNILDI_06470 0.79 -0.2
1,292,937 + nrfD BNILDI_06470 0.79 -0.4
1,292,938 - nrfD BNILDI_06470 0.80 -0.1
1,293,131 + -0.1
1,293,160 + -0.3
1,293,160 + +0.7
1,293,161 - +0.2
1,293,161 - +0.3

Or see this region's nucleotide sequence