Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metL and metF are separated by 348 nucleotides metF and katG are separated by 328 nucleotides
BNILDI_05720: metL - bifunctional aspartate kinase/homoserine dehydrogenase II, at 1,129,390 to 1,131,822
metL
BNILDI_05725: metF - methylenetetrahydrofolate reductase, at 1,132,171 to 1,133,061
metF
BNILDI_05730: katG - catalase/peroxidase HPI, at 1,133,390 to 1,135,570
katG
Position (kb)
1132
1133
1134 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1131.185 kb on - strand, within metL at 1131.223 kb on - strand, within metL at 1131.223 kb on - strand, within metL at 1131.255 kb on + strand, within metL at 1131.255 kb on + strand, within metL at 1131.256 kb on - strand, within metL at 1131.407 kb on + strand, within metL at 1131.408 kb on - strand, within metL at 1131.446 kb on - strand, within metL at 1131.602 kb on - strand at 1131.820 kb on + strand at 1131.835 kb on - strand at 1131.945 kb on + strand at 1131.946 kb on - strand at 1131.950 kb on - strand at 1131.950 kb on - strand at 1131.950 kb on - strand at 1131.950 kb on - strand at 1131.957 kb on + strand at 1131.964 kb on + strand at 1131.964 kb on + strand at 1131.964 kb on + strand at 1131.965 kb on - strand at 1131.965 kb on - strand at 1132.009 kb on - strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.141 kb on + strand at 1132.142 kb on - strand at 1132.142 kb on - strand at 1132.142 kb on - strand at 1132.142 kb on - strand at 1132.142 kb on - strand at 1132.143 kb on + strand at 1132.143 kb on + strand at 1132.143 kb on + strand at 1132.144 kb on - strand at 1132.144 kb on - strand at 1132.144 kb on - strand at 1132.147 kb on + strand at 1132.147 kb on + strand at 1132.170 kb on + strand at 1132.170 kb on + strand at 1132.171 kb on - strand at 1132.349 kb on - strand, within metF at 1132.349 kb on - strand, within metF at 1132.377 kb on + strand, within metF at 1132.393 kb on - strand, within metF at 1132.393 kb on - strand, within metF at 1132.437 kb on + strand, within metF at 1132.437 kb on + strand, within metF at 1132.437 kb on + strand, within metF at 1132.438 kb on - strand, within metF at 1132.548 kb on + strand, within metF at 1132.548 kb on + strand, within metF at 1132.561 kb on + strand, within metF at 1132.561 kb on + strand, within metF at 1132.561 kb on + strand, within metF at 1132.562 kb on - strand, within metF at 1132.562 kb on - strand, within metF at 1132.741 kb on + strand, within metF at 1132.742 kb on - strand, within metF at 1132.821 kb on + strand, within metF at 1132.822 kb on - strand, within metF at 1132.860 kb on + strand, within metF at 1132.996 kb on - strand at 1133.002 kb on - strand at 1133.059 kb on + strand at 1133.063 kb on + strand at 1133.064 kb on - strand at 1133.147 kb on + strand at 1133.177 kb on + strand at 1133.177 kb on + strand at 1133.178 kb on - strand at 1133.178 kb on - strand at 1133.178 kb on - strand at 1133.527 kb on - strand at 1133.540 kb on - strand at 1133.676 kb on - strand, within katG at 1133.720 kb on + strand, within katG at 1133.721 kb on - strand, within katG at 1133.799 kb on + strand, within katG at 1133.799 kb on + strand, within katG at 1133.799 kb on + strand, within katG at 1133.800 kb on - strand, within katG at 1133.800 kb on - strand, within katG at 1133.800 kb on - strand, within katG at 1133.891 kb on - strand, within katG at 1133.974 kb on + strand, within katG at 1133.974 kb on + strand, within katG at 1133.974 kb on + strand, within katG
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,131,185 - metL BNILDI_05720 0.74 -0.1 1,131,223 - metL BNILDI_05720 0.75 -1.0 1,131,223 - metL BNILDI_05720 0.75 -1.2 1,131,255 + metL BNILDI_05720 0.77 -0.1 1,131,255 + metL BNILDI_05720 0.77 -2.2 1,131,256 - metL BNILDI_05720 0.77 +0.2 1,131,407 + metL BNILDI_05720 0.83 -0.4 1,131,408 - metL BNILDI_05720 0.83 -0.8 1,131,446 - metL BNILDI_05720 0.85 +0.8 1,131,602 - +0.0 1,131,820 + -0.1 1,131,835 - +0.8 1,131,945 + -2.6 1,131,946 - +1.7 1,131,950 - +0.5 1,131,950 - +2.2 1,131,950 - +0.5 1,131,950 - -0.3 1,131,957 + +0.8 1,131,964 + +0.5 1,131,964 + -0.1 1,131,964 + -1.3 1,131,965 - +3.8 1,131,965 - -0.1 1,132,009 - +0.3 1,132,141 + +0.3 1,132,141 + +1.7 1,132,141 + +0.4 1,132,141 + -0.5 1,132,141 + +0.5 1,132,141 + +0.1 1,132,141 + +0.7 1,132,142 - -2.2 1,132,142 - -3.9 1,132,142 - -4.2 1,132,142 - -4.5 1,132,142 - -3.7 1,132,143 + -0.9 1,132,143 + -0.3 1,132,143 + -0.4 1,132,144 - -1.9 1,132,144 - -3.6 1,132,144 - -5.0 1,132,147 + +0.3 1,132,147 + -1.2 1,132,170 + -5.3 1,132,170 + -3.3 1,132,171 - -3.8 1,132,349 - metF BNILDI_05725 0.20 -3.3 1,132,349 - metF BNILDI_05725 0.20 -1.6 1,132,377 + metF BNILDI_05725 0.23 -4.3 1,132,393 - metF BNILDI_05725 0.25 -3.2 1,132,393 - metF BNILDI_05725 0.25 -4.7 1,132,437 + metF BNILDI_05725 0.30 -2.4 1,132,437 + metF BNILDI_05725 0.30 -2.9 1,132,437 + metF BNILDI_05725 0.30 -5.0 1,132,438 - metF BNILDI_05725 0.30 -4.0 1,132,548 + metF BNILDI_05725 0.42 -2.0 1,132,548 + metF BNILDI_05725 0.42 -0.6 1,132,561 + metF BNILDI_05725 0.44 -3.0 1,132,561 + metF BNILDI_05725 0.44 -2.1 1,132,561 + metF BNILDI_05725 0.44 -5.1 1,132,562 - metF BNILDI_05725 0.44 -2.6 1,132,562 - metF BNILDI_05725 0.44 -4.5 1,132,741 + metF BNILDI_05725 0.64 -4.8 1,132,742 - metF BNILDI_05725 0.64 -2.8 1,132,821 + metF BNILDI_05725 0.73 -3.0 1,132,822 - metF BNILDI_05725 0.73 -3.2 1,132,860 + metF BNILDI_05725 0.77 -3.6 1,132,996 - -2.8 1,133,002 - -5.5 1,133,059 + -1.1 1,133,063 + -0.9 1,133,064 - +0.9 1,133,147 + -2.2 1,133,177 + -0.4 1,133,177 + +0.3 1,133,178 - +0.7 1,133,178 - +0.4 1,133,178 - -0.5 1,133,527 - -0.4 1,133,540 - -0.1 1,133,676 - katG BNILDI_05730 0.13 +0.9 1,133,720 + katG BNILDI_05730 0.15 -0.9 1,133,721 - katG BNILDI_05730 0.15 -0.1 1,133,799 + katG BNILDI_05730 0.19 +0.4 1,133,799 + katG BNILDI_05730 0.19 -0.1 1,133,799 + katG BNILDI_05730 0.19 +0.1 1,133,800 - katG BNILDI_05730 0.19 +0.8 1,133,800 - katG BNILDI_05730 0.19 +0.9 1,133,800 - katG BNILDI_05730 0.19 +1.0 1,133,891 - katG BNILDI_05730 0.23 +0.2 1,133,974 + katG BNILDI_05730 0.27 +0.5 1,133,974 + katG BNILDI_05730 0.27 +0.2 1,133,974 + katG BNILDI_05730 0.27 -0.1
Or see this region's nucleotide sequence