Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsC and lptC overlap by 4 nucleotides lptC and lptA overlap by 32 nucleotides lptA and lptB are separated by 6 nucleotides lptB and rpoN are separated by 47 nucleotides
BNILDI_01775: kdsC - 3-deoxy-manno-octulosonate-8-phosphatase KdsC, at 346,647 to 347,213
kdsC
BNILDI_01780: lptC - LPS export ABC transporter periplasmic protein LptC, at 347,210 to 347,785
lptC
BNILDI_01785: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 347,754 to 348,311
lptA
BNILDI_01790: lptB - LPS export ABC transporter ATP-binding protein, at 348,318 to 349,043
lptB
BNILDI_01795: rpoN - RNA polymerase factor sigma-54, at 349,091 to 350,524
rpoN
Position (kb) 347
348
349 Strain fitness (log2 ratio) -1
0
1
2
3 at 346.773 kb on + strand, within kdsC at 346.773 kb on + strand, within kdsC at 346.773 kb on + strand, within kdsC at 346.932 kb on + strand, within kdsC at 346.932 kb on + strand, within kdsC at 346.932 kb on + strand, within kdsC at 347.161 kb on + strand at 347.161 kb on + strand at 347.161 kb on + strand at 347.164 kb on + strand at 347.638 kb on + strand, within lptC at 347.638 kb on + strand, within lptC at 347.663 kb on + strand, within lptC at 347.663 kb on + strand, within lptC at 347.663 kb on + strand, within lptC
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 346,773 + kdsC BNILDI_01775 0.22 +3.4 346,773 + kdsC BNILDI_01775 0.22 -1.5 346,773 + kdsC BNILDI_01775 0.22 -0.1 346,932 + kdsC BNILDI_01775 0.50 +0.9 346,932 + kdsC BNILDI_01775 0.50 +1.0 346,932 + kdsC BNILDI_01775 0.50 -0.4 347,161 + -1.0 347,161 + +0.0 347,161 + +0.6 347,164 + -1.5 347,638 + lptC BNILDI_01780 0.74 -1.3 347,638 + lptC BNILDI_01780 0.74 +1.6 347,663 + lptC BNILDI_01780 0.79 -1.3 347,663 + lptC BNILDI_01780 0.79 -0.1 347,663 + lptC BNILDI_01780 0.79 -1.1
Or see this region's nucleotide sequence