Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutE and eutJ are separated by 10 nucleotides eutJ and eutG overlap by 11 nucleotides
HEPCGN_21215: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 2,997,064 to 2,998,467
eutE
HEPCGN_21220: eutJ - ethanolamine utilization protein EutJ, at 2,998,478 to 2,999,314
eutJ
HEPCGN_21225: eutG - ethanolamine utilization ethanol dehydrogenase EutG, at 2,999,304 to 3,000,491
eutG
Position (kb)
2998
2999
3000 Strain fitness (log2 ratio)
-2
-1
0
1 at 2997.493 kb on - strand, within eutE at 2997.493 kb on - strand, within eutE at 2997.725 kb on + strand, within eutE at 2997.726 kb on - strand, within eutE at 2997.751 kb on - strand, within eutE at 2997.884 kb on + strand, within eutE at 2998.113 kb on - strand, within eutE at 2998.440 kb on - strand at 2998.442 kb on + strand at 2998.798 kb on + strand, within eutJ at 2999.020 kb on + strand, within eutJ at 2999.174 kb on - strand, within eutJ at 2999.174 kb on - strand, within eutJ at 2999.302 kb on - strand at 2999.302 kb on - strand at 2999.304 kb on - strand at 2999.375 kb on + strand at 2999.375 kb on + strand at 2999.376 kb on - strand at 2999.376 kb on - strand at 2999.376 kb on - strand at 2999.516 kb on + strand, within eutG at 2999.703 kb on - strand, within eutG at 2999.814 kb on - strand, within eutG at 2999.845 kb on + strand, within eutG at 2999.964 kb on + strand, within eutG at 2999.964 kb on + strand, within eutG at 3000.054 kb on + strand, within eutG at 3000.101 kb on + strand, within eutG at 3000.102 kb on - strand, within eutG at 3000.102 kb on - strand, within eutG at 3000.179 kb on + strand, within eutG at 3000.203 kb on + strand, within eutG at 3000.203 kb on + strand, within eutG at 3000.204 kb on - strand, within eutG at 3000.204 kb on - strand, within eutG at 3000.231 kb on - strand, within eutG at 3000.308 kb on + strand, within eutG at 3000.309 kb on - strand, within eutG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,997,493 - eutE HEPCGN_21215 0.31 +0.3 2,997,493 - eutE HEPCGN_21215 0.31 +0.8 2,997,725 + eutE HEPCGN_21215 0.47 +1.0 2,997,726 - eutE HEPCGN_21215 0.47 +0.3 2,997,751 - eutE HEPCGN_21215 0.49 -0.1 2,997,884 + eutE HEPCGN_21215 0.58 +0.5 2,998,113 - eutE HEPCGN_21215 0.75 -0.3 2,998,440 - -0.5 2,998,442 + +0.5 2,998,798 + eutJ HEPCGN_21220 0.38 +0.2 2,999,020 + eutJ HEPCGN_21220 0.65 +0.6 2,999,174 - eutJ HEPCGN_21220 0.83 -0.9 2,999,174 - eutJ HEPCGN_21220 0.83 +0.4 2,999,302 - +1.3 2,999,302 - -0.2 2,999,304 - +0.3 2,999,375 + -0.8 2,999,375 + +0.7 2,999,376 - +0.6 2,999,376 - -0.8 2,999,376 - +0.2 2,999,516 + eutG HEPCGN_21225 0.18 +0.3 2,999,703 - eutG HEPCGN_21225 0.34 -0.2 2,999,814 - eutG HEPCGN_21225 0.43 -1.9 2,999,845 + eutG HEPCGN_21225 0.46 +0.3 2,999,964 + eutG HEPCGN_21225 0.56 -0.2 2,999,964 + eutG HEPCGN_21225 0.56 -0.2 3,000,054 + eutG HEPCGN_21225 0.63 -0.5 3,000,101 + eutG HEPCGN_21225 0.67 +0.4 3,000,102 - eutG HEPCGN_21225 0.67 +0.4 3,000,102 - eutG HEPCGN_21225 0.67 +0.6 3,000,179 + eutG HEPCGN_21225 0.74 +0.0 3,000,203 + eutG HEPCGN_21225 0.76 +0.1 3,000,203 + eutG HEPCGN_21225 0.76 +0.1 3,000,204 - eutG HEPCGN_21225 0.76 +1.2 3,000,204 - eutG HEPCGN_21225 0.76 +0.2 3,000,231 - eutG HEPCGN_21225 0.78 -0.4 3,000,308 + eutG HEPCGN_21225 0.85 -0.3 3,000,309 - eutG HEPCGN_21225 0.85 -0.3
Or see this region's nucleotide sequence