Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pta and eutM overlap by 4 nucleotides eutM and eutN are separated by 105 nucleotides eutN and eutE are separated by 11 nucleotides eutE and eutJ are separated by 10 nucleotides eutJ and eutG overlap by 11 nucleotides
HEPCGN_21200: pta - phosphate acetyltransferase, at 2,995,311 to 2,996,327
pta
HEPCGN_21205: eutM - ethanolamine utilization microcompartment protein EutM, at 2,996,324 to 2,996,659
eutM
HEPCGN_21210: eutN - ethanolamine utilization microcompartment protein EutN, at 2,996,765 to 2,997,052
eutN
HEPCGN_21215: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 2,997,064 to 2,998,467
eutE
HEPCGN_21220: eutJ - ethanolamine utilization protein EutJ, at 2,998,478 to 2,999,314
eutJ
HEPCGN_21225: eutG - ethanolamine utilization ethanol dehydrogenase EutG, at 2,999,304 to 3,000,491
eutG
Position (kb)
2997
2998
2999 Strain fitness (log2 ratio)
-1
0
1
2 at 2996.083 kb on + strand, within pta at 2996.189 kb on - strand, within pta at 2996.377 kb on - strand, within eutM at 2996.377 kb on - strand, within eutM at 2996.434 kb on + strand, within eutM at 2996.465 kb on - strand, within eutM at 2996.489 kb on - strand, within eutM at 2996.580 kb on + strand, within eutM at 2996.581 kb on - strand, within eutM at 2996.711 kb on - strand at 2996.727 kb on - strand at 2996.765 kb on - strand at 2997.050 kb on + strand at 2997.051 kb on - strand at 2997.078 kb on + strand at 2997.102 kb on - strand at 2997.225 kb on - strand, within eutE at 2997.225 kb on - strand, within eutE at 2997.236 kb on + strand, within eutE at 2997.237 kb on - strand, within eutE at 2997.422 kb on + strand, within eutE at 2997.423 kb on - strand, within eutE at 2997.493 kb on - strand, within eutE at 2997.493 kb on - strand, within eutE at 2997.725 kb on + strand, within eutE at 2997.726 kb on - strand, within eutE at 2997.751 kb on - strand, within eutE at 2997.884 kb on + strand, within eutE at 2998.113 kb on - strand, within eutE at 2998.440 kb on - strand at 2998.442 kb on + strand at 2998.798 kb on + strand, within eutJ at 2999.020 kb on + strand, within eutJ at 2999.174 kb on - strand, within eutJ at 2999.174 kb on - strand, within eutJ at 2999.302 kb on - strand at 2999.302 kb on - strand at 2999.304 kb on - strand at 2999.375 kb on + strand at 2999.375 kb on + strand at 2999.376 kb on - strand at 2999.376 kb on - strand at 2999.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,996,083 + pta HEPCGN_21200 0.76 -1.4 2,996,189 - pta HEPCGN_21200 0.86 -1.1 2,996,377 - eutM HEPCGN_21205 0.16 -0.3 2,996,377 - eutM HEPCGN_21205 0.16 +2.5 2,996,434 + eutM HEPCGN_21205 0.33 -0.6 2,996,465 - eutM HEPCGN_21205 0.42 +0.8 2,996,489 - eutM HEPCGN_21205 0.49 +0.3 2,996,580 + eutM HEPCGN_21205 0.76 +0.5 2,996,581 - eutM HEPCGN_21205 0.76 -0.3 2,996,711 - -0.9 2,996,727 - +0.4 2,996,765 - +0.2 2,997,050 + +0.8 2,997,051 - +1.0 2,997,078 + +0.6 2,997,102 - +0.7 2,997,225 - eutE HEPCGN_21215 0.11 +0.9 2,997,225 - eutE HEPCGN_21215 0.11 -1.3 2,997,236 + eutE HEPCGN_21215 0.12 +0.6 2,997,237 - eutE HEPCGN_21215 0.12 +0.2 2,997,422 + eutE HEPCGN_21215 0.25 +0.0 2,997,423 - eutE HEPCGN_21215 0.26 +2.6 2,997,493 - eutE HEPCGN_21215 0.31 +0.3 2,997,493 - eutE HEPCGN_21215 0.31 +0.8 2,997,725 + eutE HEPCGN_21215 0.47 +1.0 2,997,726 - eutE HEPCGN_21215 0.47 +0.3 2,997,751 - eutE HEPCGN_21215 0.49 -0.1 2,997,884 + eutE HEPCGN_21215 0.58 +0.5 2,998,113 - eutE HEPCGN_21215 0.75 -0.3 2,998,440 - -0.5 2,998,442 + +0.5 2,998,798 + eutJ HEPCGN_21220 0.38 +0.2 2,999,020 + eutJ HEPCGN_21220 0.65 +0.6 2,999,174 - eutJ HEPCGN_21220 0.83 -0.9 2,999,174 - eutJ HEPCGN_21220 0.83 +0.4 2,999,302 - +1.3 2,999,302 - -0.2 2,999,304 - +0.3 2,999,375 + -0.8 2,999,375 + +0.7 2,999,376 - +0.6 2,999,376 - -0.8 2,999,376 - +0.2
Or see this region's nucleotide sequence