Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadK and grpE are separated by 3 nucleotides grpE and yfjD are separated by 54 nucleotides yfjD and ypjD are separated by 65 nucleotides
HEPCGN_20270: nadK - NAD(+) kinase, at 2,799,910 to 2,800,716
nadK
HEPCGN_20275: grpE - nucleotide exchange factor GrpE, at 2,800,720 to 2,801,505
grpE
HEPCGN_20280: yfjD - UPF0053 inner membrane protein YfjD, at 2,801,560 to 2,802,801
yfjD
HEPCGN_20285: ypjD - Inner membrane protein YpjD, at 2,802,867 to 2,803,733
ypjD
Position (kb)
2801
2802
2803 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2801.519 kb on - strand at 2801.562 kb on - strand at 2801.613 kb on + strand at 2801.614 kb on - strand at 2801.614 kb on - strand at 2801.619 kb on + strand at 2801.620 kb on - strand at 2801.629 kb on + strand at 2801.652 kb on - strand at 2801.725 kb on - strand, within yfjD at 2801.725 kb on - strand, within yfjD at 2801.725 kb on - strand, within yfjD at 2801.743 kb on + strand, within yfjD at 2802.029 kb on - strand, within yfjD at 2802.097 kb on - strand, within yfjD at 2802.259 kb on + strand, within yfjD at 2802.388 kb on + strand, within yfjD at 2802.388 kb on + strand, within yfjD at 2802.600 kb on + strand, within yfjD at 2802.601 kb on - strand, within yfjD at 2802.624 kb on + strand, within yfjD at 2802.625 kb on - strand, within yfjD at 2802.676 kb on - strand, within yfjD at 2802.711 kb on + strand at 2802.783 kb on - strand at 2802.825 kb on + strand at 2802.825 kb on + strand at 2802.825 kb on + strand at 2802.826 kb on - strand at 2802.826 kb on - strand at 2802.826 kb on - strand at 2802.826 kb on - strand at 2802.998 kb on + strand, within ypjD at 2802.999 kb on - strand, within ypjD at 2803.000 kb on + strand, within ypjD at 2803.001 kb on - strand, within ypjD at 2803.001 kb on - strand, within ypjD at 2803.052 kb on + strand, within ypjD at 2803.052 kb on + strand, within ypjD at 2803.053 kb on - strand, within ypjD at 2803.056 kb on + strand, within ypjD at 2803.069 kb on + strand, within ypjD at 2803.070 kb on - strand, within ypjD at 2803.070 kb on - strand, within ypjD at 2803.099 kb on - strand, within ypjD at 2803.137 kb on - strand, within ypjD at 2803.202 kb on - strand, within ypjD at 2803.202 kb on - strand, within ypjD at 2803.318 kb on + strand, within ypjD at 2803.319 kb on - strand, within ypjD at 2803.319 kb on - strand, within ypjD at 2803.369 kb on + strand, within ypjD at 2803.427 kb on + strand, within ypjD at 2803.556 kb on + strand, within ypjD at 2803.658 kb on + strand at 2803.659 kb on - strand at 2803.659 kb on - strand at 2803.661 kb on + strand at 2803.662 kb on - strand at 2803.662 kb on - strand at 2803.700 kb on + strand at 2803.700 kb on + strand at 2803.733 kb on + strand at 2803.733 kb on + strand at 2803.733 kb on + strand at 2803.756 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,801,519 - +0.8 2,801,562 - +0.5 2,801,613 + -0.5 2,801,614 - +0.1 2,801,614 - -0.1 2,801,619 + -1.3 2,801,620 - +0.5 2,801,629 + +0.9 2,801,652 - -0.8 2,801,725 - yfjD HEPCGN_20280 0.13 -0.5 2,801,725 - yfjD HEPCGN_20280 0.13 -0.7 2,801,725 - yfjD HEPCGN_20280 0.13 +0.3 2,801,743 + yfjD HEPCGN_20280 0.15 -0.2 2,802,029 - yfjD HEPCGN_20280 0.38 -0.4 2,802,097 - yfjD HEPCGN_20280 0.43 +0.3 2,802,259 + yfjD HEPCGN_20280 0.56 -2.3 2,802,388 + yfjD HEPCGN_20280 0.67 -1.3 2,802,388 + yfjD HEPCGN_20280 0.67 -2.2 2,802,600 + yfjD HEPCGN_20280 0.84 +0.5 2,802,601 - yfjD HEPCGN_20280 0.84 +0.3 2,802,624 + yfjD HEPCGN_20280 0.86 +0.2 2,802,625 - yfjD HEPCGN_20280 0.86 -0.1 2,802,676 - yfjD HEPCGN_20280 0.90 -0.1 2,802,711 + -0.0 2,802,783 - +0.1 2,802,825 + +0.3 2,802,825 + -0.5 2,802,825 + +0.3 2,802,826 - +0.2 2,802,826 - -0.5 2,802,826 - +1.2 2,802,826 - -0.4 2,802,998 + ypjD HEPCGN_20285 0.15 -1.3 2,802,999 - ypjD HEPCGN_20285 0.15 -0.2 2,803,000 + ypjD HEPCGN_20285 0.15 +0.3 2,803,001 - ypjD HEPCGN_20285 0.15 -2.8 2,803,001 - ypjD HEPCGN_20285 0.15 +1.7 2,803,052 + ypjD HEPCGN_20285 0.21 -0.5 2,803,052 + ypjD HEPCGN_20285 0.21 +0.5 2,803,053 - ypjD HEPCGN_20285 0.21 -0.0 2,803,056 + ypjD HEPCGN_20285 0.22 -0.4 2,803,069 + ypjD HEPCGN_20285 0.23 +0.9 2,803,070 - ypjD HEPCGN_20285 0.23 -0.1 2,803,070 - ypjD HEPCGN_20285 0.23 +0.4 2,803,099 - ypjD HEPCGN_20285 0.27 +0.8 2,803,137 - ypjD HEPCGN_20285 0.31 -0.8 2,803,202 - ypjD HEPCGN_20285 0.39 -0.3 2,803,202 - ypjD HEPCGN_20285 0.39 +1.6 2,803,318 + ypjD HEPCGN_20285 0.52 -0.3 2,803,319 - ypjD HEPCGN_20285 0.52 +0.6 2,803,319 - ypjD HEPCGN_20285 0.52 -0.2 2,803,369 + ypjD HEPCGN_20285 0.58 -0.3 2,803,427 + ypjD HEPCGN_20285 0.65 -0.5 2,803,556 + ypjD HEPCGN_20285 0.79 -0.6 2,803,658 + +0.2 2,803,659 - -0.6 2,803,659 - +0.4 2,803,661 + +0.2 2,803,662 - -0.2 2,803,662 - -0.1 2,803,700 + -0.2 2,803,700 + -0.6 2,803,733 + +0.5 2,803,733 + +0.1 2,803,733 + +0.3 2,803,756 + +0.5
Or see this region's nucleotide sequence