Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dolP and diaA are separated by 9 nucleotides diaA and yraN are separated by 19 nucleotides yraN and lpoA overlap by 43 nucleotides
HEPCGN_17090: dolP - division/outer membrane stress-associated lipid-binding lipoprotein, at 2,164,784 to 2,165,359
dolP
HEPCGN_17095: diaA - DnaA initiator-associating protein DiaA, at 2,165,369 to 2,165,959
diaA
HEPCGN_17100: yraN - YraN family protein, at 2,165,979 to 2,166,374
yraN
HEPCGN_17105: lpoA - penicillin-binding protein activator LpoA, at 2,166,332 to 2,168,368
lpoA
Position (kb)
2165
2166
2167 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2165.009 kb on - strand, within dolP at 2165.037 kb on + strand, within dolP at 2165.038 kb on - strand, within dolP at 2165.038 kb on - strand, within dolP at 2165.216 kb on - strand, within dolP at 2165.361 kb on + strand at 2165.361 kb on + strand at 2165.362 kb on - strand at 2165.362 kb on - strand at 2165.435 kb on - strand, within diaA at 2165.435 kb on - strand, within diaA at 2165.435 kb on - strand, within diaA at 2165.459 kb on - strand, within diaA at 2165.534 kb on + strand, within diaA at 2165.582 kb on + strand, within diaA at 2165.583 kb on - strand, within diaA at 2165.624 kb on + strand, within diaA at 2165.624 kb on + strand, within diaA at 2165.624 kb on + strand, within diaA at 2165.625 kb on - strand, within diaA at 2165.625 kb on - strand, within diaA at 2165.625 kb on - strand, within diaA at 2165.678 kb on + strand, within diaA at 2165.678 kb on + strand, within diaA at 2165.679 kb on - strand, within diaA at 2165.714 kb on - strand, within diaA at 2165.724 kb on - strand, within diaA at 2165.724 kb on - strand, within diaA at 2165.738 kb on + strand, within diaA at 2165.866 kb on + strand, within diaA at 2165.866 kb on + strand, within diaA at 2165.944 kb on + strand at 2165.969 kb on + strand at 2165.970 kb on - strand at 2165.977 kb on - strand at 2165.977 kb on - strand at 2165.981 kb on - strand at 2166.006 kb on - strand at 2166.077 kb on + strand, within yraN at 2166.077 kb on + strand, within yraN at 2166.112 kb on - strand, within yraN at 2166.216 kb on + strand, within yraN at 2166.333 kb on + strand at 2166.347 kb on + strand at 2166.363 kb on + strand at 2166.363 kb on + strand at 2166.364 kb on - strand at 2166.368 kb on + strand at 2166.368 kb on + strand at 2166.378 kb on - strand at 2166.389 kb on - strand at 2166.507 kb on + strand at 2166.507 kb on + strand at 2166.508 kb on - strand at 2166.508 kb on - strand at 2166.526 kb on - strand at 2166.669 kb on + strand, within lpoA at 2166.670 kb on - strand, within lpoA at 2166.915 kb on - strand, within lpoA at 2167.225 kb on + strand, within lpoA at 2167.225 kb on + strand, within lpoA at 2167.225 kb on + strand, within lpoA at 2167.264 kb on + strand, within lpoA at 2167.295 kb on + strand, within lpoA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,165,009 - dolP HEPCGN_17090 0.39 +0.2 2,165,037 + dolP HEPCGN_17090 0.44 +0.3 2,165,038 - dolP HEPCGN_17090 0.44 -1.0 2,165,038 - dolP HEPCGN_17090 0.44 -0.4 2,165,216 - dolP HEPCGN_17090 0.75 +0.5 2,165,361 + +0.3 2,165,361 + +0.1 2,165,362 - -0.0 2,165,362 - +0.3 2,165,435 - diaA HEPCGN_17095 0.11 -1.3 2,165,435 - diaA HEPCGN_17095 0.11 +1.6 2,165,435 - diaA HEPCGN_17095 0.11 +0.4 2,165,459 - diaA HEPCGN_17095 0.15 +0.7 2,165,534 + diaA HEPCGN_17095 0.28 +1.0 2,165,582 + diaA HEPCGN_17095 0.36 +0.8 2,165,583 - diaA HEPCGN_17095 0.36 +0.7 2,165,624 + diaA HEPCGN_17095 0.43 -1.0 2,165,624 + diaA HEPCGN_17095 0.43 +0.4 2,165,624 + diaA HEPCGN_17095 0.43 -0.5 2,165,625 - diaA HEPCGN_17095 0.43 +0.3 2,165,625 - diaA HEPCGN_17095 0.43 -1.2 2,165,625 - diaA HEPCGN_17095 0.43 +1.0 2,165,678 + diaA HEPCGN_17095 0.52 -0.6 2,165,678 + diaA HEPCGN_17095 0.52 -1.3 2,165,679 - diaA HEPCGN_17095 0.52 +0.1 2,165,714 - diaA HEPCGN_17095 0.58 +0.8 2,165,724 - diaA HEPCGN_17095 0.60 +1.2 2,165,724 - diaA HEPCGN_17095 0.60 +1.0 2,165,738 + diaA HEPCGN_17095 0.62 +1.3 2,165,866 + diaA HEPCGN_17095 0.84 +0.4 2,165,866 + diaA HEPCGN_17095 0.84 -0.3 2,165,944 + +0.8 2,165,969 + +2.9 2,165,970 - +0.1 2,165,977 - -0.0 2,165,977 - -0.7 2,165,981 - -0.9 2,166,006 - +0.3 2,166,077 + yraN HEPCGN_17100 0.25 -0.0 2,166,077 + yraN HEPCGN_17100 0.25 +1.7 2,166,112 - yraN HEPCGN_17100 0.34 -0.2 2,166,216 + yraN HEPCGN_17100 0.60 +0.1 2,166,333 + -0.3 2,166,347 + -0.2 2,166,363 + +0.3 2,166,363 + -0.7 2,166,364 - -0.4 2,166,368 + -0.0 2,166,368 + -0.5 2,166,378 - -0.9 2,166,389 - +1.3 2,166,507 + -0.2 2,166,507 + -1.1 2,166,508 - -0.2 2,166,508 - -0.3 2,166,526 - +0.2 2,166,669 + lpoA HEPCGN_17105 0.17 +2.0 2,166,670 - lpoA HEPCGN_17105 0.17 -0.1 2,166,915 - lpoA HEPCGN_17105 0.29 +0.2 2,167,225 + lpoA HEPCGN_17105 0.44 -0.8 2,167,225 + lpoA HEPCGN_17105 0.44 -0.2 2,167,225 + lpoA HEPCGN_17105 0.44 -0.2 2,167,264 + lpoA HEPCGN_17105 0.46 -0.2 2,167,295 + lpoA HEPCGN_17105 0.47 -0.3
Or see this region's nucleotide sequence