Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_08360 and tnp-IS629 overlap by 1 nucleotides tnp-IS629 and HEPCGN_08370 are separated by 85 nucleotides HEPCGN_08370 and panB are separated by 111 nucleotides panB and panC are separated by 11 nucleotides panC and HEPCGN_08385 are separated by 81 nucleotides
HEPCGN_08360: HEPCGN_08360 - HTH-21 domain-containing protein, at 411,532 to 412,011
_08360
HEPCGN_08365: tnp-IS629 - IS3 family IS629 transposase, at 412,011 to 412,337
tnp-IS629
HEPCGN_08370: HEPCGN_08370 - Fimbrial domain-containing protein, at 412,423 to 412,854
_08370
HEPCGN_08375: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 412,966 to 413,760
panB
HEPCGN_08380: panC - pantoate--beta-alanine ligase, at 413,772 to 414,623
panC
HEPCGN_08385: HEPCGN_08385 - transposase, at 414,705 to 414,914
_08385
Position (kb) 412
413
414 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 412.364 kb on + strand at 412.432 kb on + strand at 412.432 kb on + strand at 412.432 kb on + strand at 412.433 kb on - strand at 412.433 kb on - strand at 412.512 kb on + strand, within HEPCGN_08370 at 412.527 kb on - strand, within HEPCGN_08370 at 412.555 kb on - strand, within HEPCGN_08370 at 412.626 kb on - strand, within HEPCGN_08370 at 412.626 kb on - strand, within HEPCGN_08370 at 412.646 kb on + strand, within HEPCGN_08370 at 412.647 kb on - strand, within HEPCGN_08370 at 412.689 kb on + strand, within HEPCGN_08370 at 412.690 kb on - strand, within HEPCGN_08370 at 412.692 kb on + strand, within HEPCGN_08370 at 412.748 kb on + strand, within HEPCGN_08370 at 412.748 kb on + strand, within HEPCGN_08370 at 412.748 kb on + strand, within HEPCGN_08370 at 412.749 kb on - strand, within HEPCGN_08370 at 412.749 kb on - strand, within HEPCGN_08370 at 412.754 kb on - strand, within HEPCGN_08370 at 412.754 kb on - strand, within HEPCGN_08370 at 412.842 kb on + strand at 412.846 kb on + strand at 412.847 kb on - strand at 412.855 kb on + strand at 412.858 kb on + strand at 412.862 kb on - strand at 412.896 kb on + strand at 412.925 kb on + strand at 412.948 kb on + strand at 412.948 kb on + strand at 412.949 kb on - strand at 412.966 kb on - strand at 413.046 kb on + strand, within panB at 413.047 kb on - strand, within panB at 413.047 kb on - strand, within panB at 413.079 kb on + strand, within panB at 413.227 kb on - strand, within panB at 413.227 kb on - strand, within panB at 413.474 kb on + strand, within panB at 413.640 kb on + strand, within panB at 413.641 kb on - strand, within panB at 413.704 kb on + strand at 413.705 kb on - strand at 413.706 kb on + strand at 413.707 kb on - strand at 413.707 kb on - strand at 413.776 kb on + strand at 413.777 kb on - strand at 413.777 kb on - strand at 413.780 kb on + strand at 413.835 kb on - strand at 413.835 kb on - strand at 413.835 kb on - strand at 414.026 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.093 kb on + strand, within panC at 414.094 kb on - strand, within panC at 414.266 kb on + strand, within panC at 414.302 kb on + strand, within panC at 414.384 kb on + strand, within panC at 414.385 kb on - strand, within panC at 414.394 kb on + strand, within panC at 414.447 kb on - strand, within panC at 414.498 kb on - strand, within panC at 414.557 kb on - strand at 414.624 kb on + strand at 414.624 kb on + strand at 414.625 kb on - strand at 414.629 kb on - strand at 414.629 kb on - strand at 414.658 kb on - strand at 414.687 kb on - strand at 414.698 kb on - strand at 414.698 kb on - strand at 414.698 kb on - strand at 414.706 kb on + strand at 414.707 kb on - strand at 414.716 kb on + strand at 414.716 kb on + strand at 414.721 kb on - strand at 414.725 kb on + strand at 414.745 kb on - strand, within HEPCGN_08385 at 414.745 kb on - strand, within HEPCGN_08385
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 412,364 + -0.1 412,432 + +0.0 412,432 + +0.2 412,432 + -0.3 412,433 - -0.1 412,433 - -0.6 412,512 + HEPCGN_08370 0.21 -0.4 412,527 - HEPCGN_08370 0.24 +0.3 412,555 - HEPCGN_08370 0.31 +0.3 412,626 - HEPCGN_08370 0.47 -0.1 412,626 - HEPCGN_08370 0.47 +0.3 412,646 + HEPCGN_08370 0.52 -0.1 412,647 - HEPCGN_08370 0.52 -0.2 412,689 + HEPCGN_08370 0.62 -0.4 412,690 - HEPCGN_08370 0.62 +0.2 412,692 + HEPCGN_08370 0.62 +0.1 412,748 + HEPCGN_08370 0.75 -2.5 412,748 + HEPCGN_08370 0.75 +0.7 412,748 + HEPCGN_08370 0.75 -0.8 412,749 - HEPCGN_08370 0.75 -0.3 412,749 - HEPCGN_08370 0.75 -2.6 412,754 - HEPCGN_08370 0.77 -0.4 412,754 - HEPCGN_08370 0.77 +0.5 412,842 + +0.1 412,846 + +0.3 412,847 - +0.5 412,855 + -0.1 412,858 + -0.7 412,862 - -0.4 412,896 + -0.0 412,925 + -0.5 412,948 + +0.5 412,948 + +0.6 412,949 - -0.2 412,966 - -0.9 413,046 + panB HEPCGN_08375 0.10 -0.6 413,047 - panB HEPCGN_08375 0.10 -1.1 413,047 - panB HEPCGN_08375 0.10 +0.8 413,079 + panB HEPCGN_08375 0.14 -2.0 413,227 - panB HEPCGN_08375 0.33 -1.0 413,227 - panB HEPCGN_08375 0.33 -2.1 413,474 + panB HEPCGN_08375 0.64 -0.6 413,640 + panB HEPCGN_08375 0.85 -1.0 413,641 - panB HEPCGN_08375 0.85 -0.7 413,704 + -1.1 413,705 - -1.8 413,706 + +1.1 413,707 - -1.3 413,707 - -1.4 413,776 + -0.9 413,777 - -0.7 413,777 - -2.1 413,780 + -2.9 413,835 - -0.5 413,835 - -0.6 413,835 - +2.3 414,026 + panC HEPCGN_08380 0.30 -0.5 414,093 + panC HEPCGN_08380 0.38 -1.1 414,093 + panC HEPCGN_08380 0.38 -3.8 414,093 + panC HEPCGN_08380 0.38 +0.6 414,093 + panC HEPCGN_08380 0.38 -0.9 414,093 + panC HEPCGN_08380 0.38 -1.1 414,093 + panC HEPCGN_08380 0.38 -3.8 414,094 - panC HEPCGN_08380 0.38 -1.5 414,266 + panC HEPCGN_08380 0.58 -0.4 414,302 + panC HEPCGN_08380 0.62 -1.9 414,384 + panC HEPCGN_08380 0.72 -1.5 414,385 - panC HEPCGN_08380 0.72 -1.7 414,394 + panC HEPCGN_08380 0.73 -2.5 414,447 - panC HEPCGN_08380 0.79 -1.3 414,498 - panC HEPCGN_08380 0.85 -1.7 414,557 - +0.4 414,624 + +0.3 414,624 + -0.6 414,625 - +0.5 414,629 - +0.2 414,629 - +0.4 414,658 - +0.2 414,687 - -0.7 414,698 - -2.0 414,698 - +0.1 414,698 - +0.0 414,706 + +1.1 414,707 - +0.4 414,716 + -0.1 414,716 + +0.7 414,721 - -0.0 414,725 + +0.4 414,745 - HEPCGN_08385 0.19 +0.5 414,745 - HEPCGN_08385 0.19 -0.6
Or see this region's nucleotide sequence