Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rdgC and ykiD are separated by 77 nucleotides ykiD and HEPCGN_07220 are separated by 199 nucleotides HEPCGN_07220 and ppnP are separated by 103 nucleotides ppnP and aroM are separated by 71 nucleotides
HEPCGN_07210: rdgC - recombination-associated protein RdgC, at 179,061 to 180,038
rdgC
HEPCGN_07215: ykiD - Protein YkiD, at 180,116 to 180,199
ykiD
HEPCGN_07220: HEPCGN_07220 - hypothetical protein, at 180,399 to 180,578
_07220
HEPCGN_07225: ppnP - pyrimidine/purine nucleoside phosphorylase, at 180,682 to 180,966
ppnP
HEPCGN_07230: aroM - Protein AroM, at 181,038 to 181,715
aroM
Position (kb) 180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 179.207 kb on - strand, within rdgC at 179.223 kb on - strand, within rdgC at 179.574 kb on - strand, within rdgC at 179.591 kb on + strand, within rdgC at 179.623 kb on + strand, within rdgC at 179.817 kb on - strand, within rdgC at 180.036 kb on + strand at 180.037 kb on - strand at 180.048 kb on + strand at 180.113 kb on + strand at 180.114 kb on - strand at 180.114 kb on - strand at 180.114 kb on - strand at 180.155 kb on - strand, within ykiD at 180.158 kb on - strand, within ykiD at 180.167 kb on - strand, within ykiD at 180.167 kb on - strand, within ykiD at 180.167 kb on - strand, within ykiD at 180.173 kb on + strand, within ykiD at 180.420 kb on + strand, within HEPCGN_07220 at 180.420 kb on + strand, within HEPCGN_07220 at 180.421 kb on - strand, within HEPCGN_07220 at 180.421 kb on - strand, within HEPCGN_07220 at 180.580 kb on + strand at 180.580 kb on + strand at 180.581 kb on - strand at 180.615 kb on + strand at 180.619 kb on - strand at 180.684 kb on - strand at 180.701 kb on + strand at 180.863 kb on + strand, within ppnP at 180.879 kb on + strand, within ppnP at 180.880 kb on - strand, within ppnP at 180.926 kb on + strand, within ppnP at 180.947 kb on + strand at 180.997 kb on - strand at 181.048 kb on - strand at 181.048 kb on - strand at 181.075 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 179,207 - rdgC HEPCGN_07210 0.15 +0.2 179,223 - rdgC HEPCGN_07210 0.17 +0.1 179,574 - rdgC HEPCGN_07210 0.52 +0.0 179,591 + rdgC HEPCGN_07210 0.54 +0.1 179,623 + rdgC HEPCGN_07210 0.57 -1.9 179,817 - rdgC HEPCGN_07210 0.77 +1.1 180,036 + -0.3 180,037 - -0.1 180,048 + -2.9 180,113 + +1.0 180,114 - -0.6 180,114 - -0.1 180,114 - -0.1 180,155 - ykiD HEPCGN_07215 0.46 +0.7 180,158 - ykiD HEPCGN_07215 0.50 -0.7 180,167 - ykiD HEPCGN_07215 0.61 +0.9 180,167 - ykiD HEPCGN_07215 0.61 -0.7 180,167 - ykiD HEPCGN_07215 0.61 -0.0 180,173 + ykiD HEPCGN_07215 0.68 -0.4 180,420 + HEPCGN_07220 0.12 -0.4 180,420 + HEPCGN_07220 0.12 +0.2 180,421 - HEPCGN_07220 0.12 +0.3 180,421 - HEPCGN_07220 0.12 +0.4 180,580 + +0.8 180,580 + +0.4 180,581 - +0.1 180,615 + -1.1 180,619 - -0.5 180,684 - -0.1 180,701 + +0.2 180,863 + ppnP HEPCGN_07225 0.64 +0.2 180,879 + ppnP HEPCGN_07225 0.69 -0.1 180,880 - ppnP HEPCGN_07225 0.69 +0.2 180,926 + ppnP HEPCGN_07225 0.86 -0.1 180,947 + +0.4 180,997 - +0.7 181,048 - -0.1 181,048 - -0.2 181,075 - -0.2
Or see this region's nucleotide sequence