Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides bioD and uvrB are separated by 578 nucleotides
HEPCGN_01645: bioF - 8-amino-7-oxononanoate synthase, at 252,230 to 253,384
bioF
HEPCGN_01650: bioC - malonyl-ACP O-methyltransferase BioC, at 253,371 to 254,126
bioC
HEPCGN_01655: bioD - dethiobiotin synthase, at 254,119 to 254,796
bioD
HEPCGN_01660: uvrB - excinuclease ABC subunit UvrB, at 255,375 to 257,396
uvrB
Position (kb) 254
255 Strain fitness (log2 ratio) -2
-1
0
1
2 at 253.147 kb on - strand, within bioF at 253.147 kb on - strand, within bioF at 253.163 kb on - strand, within bioF at 253.225 kb on - strand, within bioF at 253.315 kb on - strand at 253.315 kb on - strand at 253.386 kb on - strand at 253.429 kb on - strand at 253.494 kb on - strand, within bioC at 253.494 kb on - strand, within bioC at 253.580 kb on - strand, within bioC at 253.636 kb on - strand, within bioC at 253.636 kb on - strand, within bioC at 253.726 kb on + strand, within bioC at 254.092 kb on - strand at 254.271 kb on - strand, within bioD at 254.274 kb on + strand, within bioD at 254.324 kb on - strand, within bioD at 254.342 kb on - strand, within bioD at 254.342 kb on - strand, within bioD at 254.485 kb on - strand, within bioD at 254.546 kb on + strand, within bioD at 254.574 kb on + strand, within bioD at 254.575 kb on - strand, within bioD at 254.680 kb on + strand, within bioD at 254.682 kb on + strand, within bioD at 254.682 kb on + strand, within bioD at 254.683 kb on - strand, within bioD at 254.773 kb on + strand at 254.794 kb on + strand at 254.794 kb on + strand at 254.965 kb on - strand at 255.073 kb on + strand at 255.089 kb on - strand at 255.092 kb on + strand at 255.314 kb on + strand at 255.318 kb on + strand at 255.318 kb on + strand at 255.337 kb on + strand at 255.337 kb on + strand at 255.484 kb on + strand at 255.559 kb on + strand at 255.560 kb on - strand at 255.560 kb on - strand at 255.594 kb on + strand, within uvrB at 255.649 kb on - strand, within uvrB at 255.652 kb on - strand, within uvrB at 255.658 kb on - strand, within uvrB at 255.675 kb on + strand, within uvrB at 255.676 kb on - strand, within uvrB at 255.676 kb on - strand, within uvrB at 255.679 kb on + strand, within uvrB at 255.787 kb on + strand, within uvrB at 255.788 kb on - strand, within uvrB at 255.788 kb on - strand, within uvrB at 255.788 kb on - strand, within uvrB at 255.788 kb on - strand, within uvrB at 255.788 kb on - strand, within uvrB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 253,147 - bioF HEPCGN_01645 0.79 -2.3 253,147 - bioF HEPCGN_01645 0.79 +0.8 253,163 - bioF HEPCGN_01645 0.81 +0.5 253,225 - bioF HEPCGN_01645 0.86 +0.3 253,315 - +1.3 253,315 - -0.2 253,386 - -0.6 253,429 - -0.4 253,494 - bioC HEPCGN_01650 0.16 -2.0 253,494 - bioC HEPCGN_01650 0.16 -0.6 253,580 - bioC HEPCGN_01650 0.28 -0.3 253,636 - bioC HEPCGN_01650 0.35 +0.5 253,636 - bioC HEPCGN_01650 0.35 +0.6 253,726 + bioC HEPCGN_01650 0.47 +0.6 254,092 - +0.4 254,271 - bioD HEPCGN_01655 0.22 +0.6 254,274 + bioD HEPCGN_01655 0.23 -2.1 254,324 - bioD HEPCGN_01655 0.30 -0.1 254,342 - bioD HEPCGN_01655 0.33 -0.3 254,342 - bioD HEPCGN_01655 0.33 -0.5 254,485 - bioD HEPCGN_01655 0.54 -0.1 254,546 + bioD HEPCGN_01655 0.63 -0.5 254,574 + bioD HEPCGN_01655 0.67 -0.9 254,575 - bioD HEPCGN_01655 0.67 +0.4 254,680 + bioD HEPCGN_01655 0.83 -0.1 254,682 + bioD HEPCGN_01655 0.83 -0.2 254,682 + bioD HEPCGN_01655 0.83 -0.7 254,683 - bioD HEPCGN_01655 0.83 +0.2 254,773 + -1.7 254,794 + +0.7 254,794 + -0.1 254,965 - -0.2 255,073 + +0.0 255,089 - -0.4 255,092 + -0.6 255,314 + +0.4 255,318 + +0.4 255,318 + -0.3 255,337 + +0.4 255,337 + +0.7 255,484 + +0.7 255,559 + +0.4 255,560 - -0.8 255,560 - -0.9 255,594 + uvrB HEPCGN_01660 0.11 -1.4 255,649 - uvrB HEPCGN_01660 0.14 -1.6 255,652 - uvrB HEPCGN_01660 0.14 +0.3 255,658 - uvrB HEPCGN_01660 0.14 -0.2 255,675 + uvrB HEPCGN_01660 0.15 +0.7 255,676 - uvrB HEPCGN_01660 0.15 +2.5 255,676 - uvrB HEPCGN_01660 0.15 +0.6 255,679 + uvrB HEPCGN_01660 0.15 -0.3 255,787 + uvrB HEPCGN_01660 0.20 -1.4 255,788 - uvrB HEPCGN_01660 0.20 +0.1 255,788 - uvrB HEPCGN_01660 0.20 -0.2 255,788 - uvrB HEPCGN_01660 0.20 +0.1 255,788 - uvrB HEPCGN_01660 0.20 -0.5 255,788 - uvrB HEPCGN_01660 0.20 -0.1
Or see this region's nucleotide sequence