Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgU and sdhC are separated by 380 nucleotides sdhC and sdhD overlap by 7 nucleotides sdhD and sdhA overlap by 1 nucleotides
HEPCGN_01280: ybgU - Protein YbgU, at 182,192 to 182,299
ybgU
HEPCGN_01285: sdhC - succinate dehydrogenase cytochrome b556 subunit, at 182,680 to 183,084
sdhC
HEPCGN_01290: sdhD - succinate dehydrogenase membrane anchor subunit, at 183,078 to 183,425
sdhD
HEPCGN_01295: sdhA - succinate dehydrogenase flavoprotein subunit, at 183,425 to 185,191
sdhA
Position (kb) 183
184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 182.210 kb on - strand, within ybgU at 182.213 kb on - strand, within ybgU at 182.252 kb on - strand, within ybgU at 182.255 kb on + strand, within ybgU at 182.255 kb on + strand, within ybgU at 182.261 kb on - strand, within ybgU at 182.300 kb on + strand at 182.301 kb on - strand at 182.301 kb on - strand at 182.301 kb on - strand at 182.301 kb on - strand at 182.424 kb on + strand at 182.425 kb on - strand at 182.427 kb on - strand at 182.566 kb on + strand at 182.567 kb on - strand at 182.668 kb on - strand at 182.670 kb on + strand at 182.671 kb on - strand at 182.739 kb on - strand, within sdhC at 182.769 kb on - strand, within sdhC at 182.910 kb on + strand, within sdhC at 182.941 kb on + strand, within sdhC at 182.942 kb on - strand, within sdhC at 182.954 kb on + strand, within sdhC at 182.958 kb on + strand, within sdhC at 182.959 kb on - strand, within sdhC at 182.959 kb on - strand, within sdhC at 183.039 kb on + strand, within sdhC at 183.043 kb on - strand, within sdhC at 183.078 kb on - strand at 183.082 kb on + strand at 183.116 kb on - strand, within sdhD at 183.162 kb on + strand, within sdhD at 183.163 kb on - strand, within sdhD at 183.171 kb on + strand, within sdhD at 183.172 kb on - strand, within sdhD at 183.172 kb on - strand, within sdhD at 183.172 kb on - strand, within sdhD at 183.173 kb on + strand, within sdhD at 183.173 kb on + strand, within sdhD at 183.174 kb on - strand, within sdhD at 183.210 kb on + strand, within sdhD at 183.211 kb on - strand, within sdhD at 183.279 kb on - strand, within sdhD at 183.325 kb on - strand, within sdhD at 183.482 kb on - strand at 183.601 kb on + strand at 183.601 kb on + strand at 183.602 kb on - strand, within sdhA at 183.628 kb on + strand, within sdhA at 183.629 kb on - strand, within sdhA at 183.633 kb on - strand, within sdhA at 183.633 kb on - strand, within sdhA at 183.656 kb on + strand, within sdhA at 183.656 kb on + strand, within sdhA at 183.656 kb on + strand, within sdhA at 183.656 kb on + strand, within sdhA at 183.659 kb on - strand, within sdhA at 183.684 kb on - strand, within sdhA at 183.686 kb on - strand, within sdhA at 183.686 kb on - strand, within sdhA at 183.686 kb on - strand, within sdhA at 183.686 kb on - strand, within sdhA at 183.692 kb on - strand, within sdhA at 183.728 kb on - strand, within sdhA at 183.768 kb on - strand, within sdhA at 183.872 kb on + strand, within sdhA at 183.872 kb on + strand, within sdhA at 183.873 kb on - strand, within sdhA at 183.917 kb on + strand, within sdhA at 183.964 kb on + strand, within sdhA at 184.046 kb on - strand, within sdhA at 184.116 kb on + strand, within sdhA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 182,210 - ybgU HEPCGN_01280 0.17 -0.5 182,213 - ybgU HEPCGN_01280 0.19 -0.2 182,252 - ybgU HEPCGN_01280 0.56 +0.0 182,255 + ybgU HEPCGN_01280 0.58 -2.0 182,255 + ybgU HEPCGN_01280 0.58 -1.4 182,261 - ybgU HEPCGN_01280 0.64 -0.0 182,300 + +0.6 182,301 - +0.1 182,301 - +2.8 182,301 - -0.2 182,301 - +0.8 182,424 + -0.8 182,425 - -0.3 182,427 - -2.4 182,566 + +0.1 182,567 - -3.8 182,668 - -1.5 182,670 + -1.4 182,671 - -4.0 182,739 - sdhC HEPCGN_01285 0.15 -1.8 182,769 - sdhC HEPCGN_01285 0.22 -0.3 182,910 + sdhC HEPCGN_01285 0.57 -3.3 182,941 + sdhC HEPCGN_01285 0.64 -1.8 182,942 - sdhC HEPCGN_01285 0.65 -2.6 182,954 + sdhC HEPCGN_01285 0.68 -2.0 182,958 + sdhC HEPCGN_01285 0.69 -0.8 182,959 - sdhC HEPCGN_01285 0.69 -4.1 182,959 - sdhC HEPCGN_01285 0.69 -2.4 183,039 + sdhC HEPCGN_01285 0.89 -3.2 183,043 - sdhC HEPCGN_01285 0.90 -2.8 183,078 - -3.3 183,082 + -4.7 183,116 - sdhD HEPCGN_01290 0.11 -4.4 183,162 + sdhD HEPCGN_01290 0.24 -1.9 183,163 - sdhD HEPCGN_01290 0.24 -0.6 183,171 + sdhD HEPCGN_01290 0.27 +0.3 183,172 - sdhD HEPCGN_01290 0.27 -2.5 183,172 - sdhD HEPCGN_01290 0.27 -1.8 183,172 - sdhD HEPCGN_01290 0.27 -1.5 183,173 + sdhD HEPCGN_01290 0.27 -3.2 183,173 + sdhD HEPCGN_01290 0.27 +0.0 183,174 - sdhD HEPCGN_01290 0.28 -3.0 183,210 + sdhD HEPCGN_01290 0.38 -1.0 183,211 - sdhD HEPCGN_01290 0.38 -0.5 183,279 - sdhD HEPCGN_01290 0.58 -2.8 183,325 - sdhD HEPCGN_01290 0.71 -1.4 183,482 - -2.3 183,601 + -2.5 183,601 + -1.7 183,602 - sdhA HEPCGN_01295 0.10 -4.4 183,628 + sdhA HEPCGN_01295 0.11 -0.3 183,629 - sdhA HEPCGN_01295 0.12 -3.3 183,633 - sdhA HEPCGN_01295 0.12 -1.5 183,633 - sdhA HEPCGN_01295 0.12 -3.4 183,656 + sdhA HEPCGN_01295 0.13 -1.9 183,656 + sdhA HEPCGN_01295 0.13 -3.9 183,656 + sdhA HEPCGN_01295 0.13 -2.1 183,656 + sdhA HEPCGN_01295 0.13 -2.8 183,659 - sdhA HEPCGN_01295 0.13 -1.0 183,684 - sdhA HEPCGN_01295 0.15 -2.2 183,686 - sdhA HEPCGN_01295 0.15 -3.7 183,686 - sdhA HEPCGN_01295 0.15 -3.5 183,686 - sdhA HEPCGN_01295 0.15 -3.9 183,686 - sdhA HEPCGN_01295 0.15 -2.4 183,692 - sdhA HEPCGN_01295 0.15 -4.1 183,728 - sdhA HEPCGN_01295 0.17 -3.7 183,768 - sdhA HEPCGN_01295 0.19 -1.2 183,872 + sdhA HEPCGN_01295 0.25 -2.8 183,872 + sdhA HEPCGN_01295 0.25 +0.1 183,873 - sdhA HEPCGN_01295 0.25 -2.0 183,917 + sdhA HEPCGN_01295 0.28 -3.1 183,964 + sdhA HEPCGN_01295 0.31 -1.5 184,046 - sdhA HEPCGN_01295 0.35 -2.0 184,116 + sdhA HEPCGN_01295 0.39 -1.8
Or see this region's nucleotide sequence