Experiment: Matrix:pear;Phage:inactivated cocktail;Temp:28;Samp:15-8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OLJFJH_02955 and OLJFJH_02960 are separated by 60 nucleotides OLJFJH_02960 and OLJFJH_02965 overlap by 4 nucleotides OLJFJH_02965 and OLJFJH_02970 are separated by 136 nucleotides
OLJFJH_02955: OLJFJH_02955 - asparagine synthase (glutamine-hydrolyzing), at 532,453 to 534,042
_02955
OLJFJH_02960: OLJFJH_02960 - pantoate--beta-alanine ligase, at 534,103 to 535,026
_02960
OLJFJH_02965: OLJFJH_02965 - phosphoribosylamine--glycine ligase, at 535,023 to 536,276
_02965
OLJFJH_02970: OLJFJH_02970 - histidine--tRNA ligase, at 536,413 to 537,732
_02970
Position (kb) 535
536
537 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 534.536 kb on - strand, within OLJFJH_02960 at 534.875 kb on + strand, within OLJFJH_02960 at 534.875 kb on + strand, within OLJFJH_02960 at 534.876 kb on - strand, within OLJFJH_02960 at 534.897 kb on + strand, within OLJFJH_02960 at 534.898 kb on - strand, within OLJFJH_02960 at 535.083 kb on - strand at 535.083 kb on - strand at 535.159 kb on + strand, within OLJFJH_02965 at 535.558 kb on - strand, within OLJFJH_02965 at 535.621 kb on + strand, within OLJFJH_02965 at 535.621 kb on + strand, within OLJFJH_02965 at 535.657 kb on + strand, within OLJFJH_02965 at 535.894 kb on + strand, within OLJFJH_02965 at 535.937 kb on - strand, within OLJFJH_02965 at 536.158 kb on + strand at 536.708 kb on - strand, within OLJFJH_02970 at 536.708 kb on - strand, within OLJFJH_02970 at 536.812 kb on - strand, within OLJFJH_02970 at 537.041 kb on + strand, within OLJFJH_02970 at 537.072 kb on - strand, within OLJFJH_02970 at 537.146 kb on + strand, within OLJFJH_02970
Per-strain Table
Position Strand Gene LocusTag Fraction Matrix:pear;Phage:inactivated cocktail;Temp:28;Samp:15-8 remove 534,536 - OLJFJH_02960 0.47 +0.1 534,875 + OLJFJH_02960 0.84 -0.3 534,875 + OLJFJH_02960 0.84 +0.2 534,876 - OLJFJH_02960 0.84 +0.0 534,897 + OLJFJH_02960 0.86 +1.1 534,898 - OLJFJH_02960 0.86 +0.6 535,083 - +1.3 535,083 - +0.9 535,159 + OLJFJH_02965 0.11 -0.6 535,558 - OLJFJH_02965 0.43 +1.0 535,621 + OLJFJH_02965 0.48 -0.6 535,621 + OLJFJH_02965 0.48 +0.5 535,657 + OLJFJH_02965 0.51 +7.2 535,894 + OLJFJH_02965 0.69 -0.6 535,937 - OLJFJH_02965 0.73 +0.1 536,158 + +1.6 536,708 - OLJFJH_02970 0.22 +0.0 536,708 - OLJFJH_02970 0.22 +0.4 536,812 - OLJFJH_02970 0.30 -3.1 537,041 + OLJFJH_02970 0.48 -0.4 537,072 - OLJFJH_02970 0.50 -1.5 537,146 + OLJFJH_02970 0.56 +0.2
Or see this region's nucleotide sequence