Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28680

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciI and tonB are separated by 223 nucleotidestonB and yciA are separated by 39 nucleotidesyciA and yciB are separated by 104 nucleotidesyciB and yciC are separated by 29 nucleotides OKFHMN_28670: yciI - YciI family protein, at 5,502,031 to 5,502,327 yciI OKFHMN_28675: tonB - TonB system transport protein TonB, at 5,502,551 to 5,503,270 tonB OKFHMN_28680: yciA - acyl-CoA thioester hydrolase YciA, at 5,503,310 to 5,503,708 yciA OKFHMN_28685: yciB - septation protein A, at 5,503,813 to 5,504,352 yciB OKFHMN_28690: yciC - envelope biogenesis factor ElyC, at 5,504,382 to 5,505,125 yciC Position (kb) 5503 5504Strain fitness (log2 ratio) -2 -1 0 1 2at 5502.316 kb on - strandat 5502.356 kb on + strandat 5502.357 kb on - strandat 5502.529 kb on + strandat 5502.636 kb on - strand, within tonBat 5502.645 kb on + strand, within tonBat 5503.122 kb on + strand, within tonBat 5503.122 kb on + strand, within tonBat 5503.360 kb on - strand, within yciAat 5503.364 kb on - strand, within yciAat 5503.703 kb on - strandat 5503.848 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,502,316 - +0.6
5,502,356 + -0.5
5,502,357 - -0.9
5,502,529 + +0.1
5,502,636 - tonB OKFHMN_28675 0.12 -0.2
5,502,645 + tonB OKFHMN_28675 0.13 -0.1
5,503,122 + tonB OKFHMN_28675 0.79 -1.1
5,503,122 + tonB OKFHMN_28675 0.79 +1.8
5,503,360 - yciA OKFHMN_28680 0.13 -1.3
5,503,364 - yciA OKFHMN_28680 0.14 -2.4
5,503,703 - +1.3
5,503,848 + +1.8

Or see this region's nucleotide sequence