Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28300

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdadX and tnp-IS629 are separated by 337 nucleotidestnp-IS629 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and OKFHMN_28310 overlap by 35 nucleotides OKFHMN_28295: dadX - catabolic alanine racemase DadX, at 5,427,700 to 5,428,770 dadX OKFHMN_28300: tnp-IS629 - IS3 family IS629 transposase, at 5,429,108 to 5,429,434 tnp-IS629 OKFHMN_28305: tnp-IS3 - IS3 family transposase, at 5,429,434 to 5,430,321 tnp-IS3 OKFHMN_28310: OKFHMN_28310 - AMP nucleosidase, at 5,430,287 to 5,430,376 _28310 Position (kb) 5429 5430Strain fitness (log2 ratio) -1 0 1at 5428.404 kb on - strand, within dadXat 5428.471 kb on - strand, within dadXat 5430.416 kb on + strandat 5430.416 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,428,404 - dadX OKFHMN_28295 0.66 +0.4
5,428,471 - dadX OKFHMN_28295 0.72 +0.2
5,430,416 + +0.2
5,430,416 + -0.1

Or see this region's nucleotide sequence