Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27425

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlhr and rnt are separated by 92 nucleotidesrnt and OKFHMN_27430 are separated by 102 nucleotidesOKFHMN_27430 and nemA are separated by 80 nucleotides OKFHMN_27420: lhr - ATP-dependent helicase, at 5,276,991 to 5,281,607 lhr OKFHMN_27425: rnt - ribonuclease T, at 5,281,700 to 5,282,347 rnt OKFHMN_27430: OKFHMN_27430 - Lactoylglutathione lyase, at 5,282,450 to 5,282,857 _27430 OKFHMN_27435: nemA - N-ethylmaleimide reductase, at 5,282,938 to 5,284,035 nemA Position (kb) 5281 5282 5283Strain fitness (log2 ratio) -1 0 1 2 3at 5281.132 kb on + strand, within lhrat 5281.132 kb on + strand, within lhrat 5281.615 kb on - strandat 5281.649 kb on - strandat 5281.659 kb on + strandat 5281.659 kb on + strandat 5281.701 kb on + strandat 5282.452 kb on - strandat 5282.537 kb on - strand, within OKFHMN_27430at 5282.618 kb on - strand, within OKFHMN_27430at 5282.618 kb on - strand, within OKFHMN_27430at 5282.644 kb on - strand, within OKFHMN_27430at 5282.662 kb on - strand, within OKFHMN_27430at 5282.960 kb on + strandat 5282.961 kb on - strandat 5282.982 kb on - strandat 5283.086 kb on - strand, within nemAat 5283.167 kb on - strand, within nemAat 5283.190 kb on - strand, within nemAat 5283.191 kb on + strand, within nemAat 5283.225 kb on + strand, within nemAat 5283.226 kb on - strand, within nemAat 5283.226 kb on - strand, within nemA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,281,132 + lhr OKFHMN_27420 0.90 -0.3
5,281,132 + lhr OKFHMN_27420 0.90 -0.3
5,281,615 - -1.3
5,281,649 - +0.4
5,281,659 + +0.4
5,281,659 + -1.1
5,281,701 + +1.4
5,282,452 - +0.4
5,282,537 - OKFHMN_27430 0.21 -0.7
5,282,618 - OKFHMN_27430 0.41 +0.4
5,282,618 - OKFHMN_27430 0.41 +0.5
5,282,644 - OKFHMN_27430 0.48 -0.5
5,282,662 - OKFHMN_27430 0.52 +1.0
5,282,960 + -0.8
5,282,961 - -0.9
5,282,982 - +0.8
5,283,086 - nemA OKFHMN_27435 0.13 -1.0
5,283,167 - nemA OKFHMN_27435 0.21 -0.6
5,283,190 - nemA OKFHMN_27435 0.23 -0.4
5,283,191 + nemA OKFHMN_27435 0.23 +0.1
5,283,225 + nemA OKFHMN_27435 0.26 +2.9
5,283,226 - nemA OKFHMN_27435 0.26 +1.9
5,283,226 - nemA OKFHMN_27435 0.26 +0.3

Or see this region's nucleotide sequence