Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26930

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastE and spy are separated by 329 nucleotidesspy and ves are separated by 202 nucleotidesves and cho overlap by 41 nucleotides OKFHMN_26925: astE - succinylglutamate desuccinylase, at 5,183,984 to 5,184,952 astE OKFHMN_26930: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 5,185,282 to 5,185,767 spy OKFHMN_26935: ves - environmental stress-induced protein Ves, at 5,185,970 to 5,186,545 ves OKFHMN_26940: cho - excinuclease Cho, at 5,186,505 to 5,187,392 cho Position (kb) 5185 5186Strain fitness (log2 ratio) -2 -1 0 1 2at 5184.420 kb on + strand, within astEat 5184.946 kb on - strandat 5184.951 kb on - strandat 5184.984 kb on + strandat 5185.189 kb on + strandat 5185.292 kb on + strandat 5185.624 kb on - strand, within spyat 5185.894 kb on + strandat 5185.895 kb on - strandat 5185.895 kb on - strandat 5185.970 kb on - strandat 5186.205 kb on + strand, within vesat 5186.341 kb on + strand, within vesat 5186.341 kb on + strand, within vesat 5186.485 kb on + strand, within vesat 5186.486 kb on - strand, within vesat 5186.486 kb on - strand, within vesat 5186.679 kb on - strand, within cho

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,184,420 + astE OKFHMN_26925 0.45 -0.5
5,184,946 - -0.8
5,184,951 - +0.1
5,184,984 + +0.1
5,185,189 + +0.5
5,185,292 + -0.6
5,185,624 - spy OKFHMN_26930 0.70 +2.2
5,185,894 + -0.6
5,185,895 - +0.8
5,185,895 - -0.3
5,185,970 - +1.3
5,186,205 + ves OKFHMN_26935 0.41 -1.3
5,186,341 + ves OKFHMN_26935 0.64 +0.8
5,186,341 + ves OKFHMN_26935 0.64 +0.7
5,186,485 + ves OKFHMN_26935 0.89 -1.3
5,186,486 - ves OKFHMN_26935 0.90 +0.5
5,186,486 - ves OKFHMN_26935 0.90 -1.8
5,186,679 - cho OKFHMN_26940 0.20 -0.7

Or see this region's nucleotide sequence