Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuE and yeaR are separated by 172 nucleotides yeaR and yoaG are separated by 3 nucleotides yoaG and yeaQ are separated by 146 nucleotides yeaQ and yoaJ are separated by 58 nucleotides yoaJ and gAF are separated by 310 nucleotides
OKFHMN_26635: leuE - leucine efflux protein LeuE, at 5,128,056 to 5,128,694
leuE
OKFHMN_26640: yeaR - Uncharacterized protein YeaR, at 5,128,867 to 5,129,226
yeaR
OKFHMN_26645: yoaG - Protein YoaG, at 5,129,230 to 5,129,412
yoaG
OKFHMN_26650: yeaQ - UPF0410 protein YeaQ, at 5,129,559 to 5,129,807
yeaQ
OKFHMN_26655: yoaJ - protein YoaJ, at 5,129,866 to 5,129,940
yoaJ
OKFHMN_26660: gAF - GAF domain, at 5,130,251 to 5,131,099
gAF
Position (kb) 5129
5130 Strain fitness (log2 ratio) -1
0
1
2 at 5128.600 kb on + strand, within leuE at 5128.622 kb on + strand, within leuE at 5128.729 kb on + strand at 5128.809 kb on + strand at 5128.890 kb on + strand at 5129.290 kb on + strand, within yoaG at 5129.505 kb on - strand at 5129.545 kb on + strand at 5129.962 kb on + strand at 5130.069 kb on - strand at 5130.159 kb on - strand at 5130.159 kb on - strand at 5130.425 kb on - strand, within gAF
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,128,600 + leuE OKFHMN_26635 0.85 +0.9 5,128,622 + leuE OKFHMN_26635 0.89 +1.4 5,128,729 + -0.5 5,128,809 + +1.5 5,128,890 + +0.1 5,129,290 + yoaG OKFHMN_26645 0.33 +0.4 5,129,505 - +0.4 5,129,545 + -1.5 5,129,962 + +1.6 5,130,069 - +0.2 5,130,159 - +1.0 5,130,159 - +2.2 5,130,425 - gAF OKFHMN_26660 0.20 +0.5
Or see this region's nucleotide sequence