Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and yebG are separated by 133 nucleotides yebG and yebF are separated by 66 nucleotides yebF and OKFHMN_26365 overlap by 87 nucleotides OKFHMN_26365 and OKFHMN_26370 are separated by 36 nucleotides OKFHMN_26370 and yebE are separated by 59 nucleotides
OKFHMN_26350: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 5,076,529 to 5,077,707
purT
OKFHMN_26355: yebG - DNA damage-inducible protein YebG, at 5,077,841 to 5,078,131
yebG
OKFHMN_26360: yebF - Protein YebF, at 5,078,198 to 5,078,554
yebF
OKFHMN_26365: OKFHMN_26365 - hypothetical protein, at 5,078,468 to 5,078,674
_26365
OKFHMN_26370: OKFHMN_26370 - hypothetical protein, at 5,078,711 to 5,078,821
_26370
OKFHMN_26375: yebE - Inner membrane protein YebE, at 5,078,881 to 5,079,540
yebE
Position (kb) 5078
5079 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5077.476 kb on - strand, within purT at 5077.528 kb on - strand, within purT at 5077.531 kb on - strand, within purT at 5077.655 kb on - strand at 5077.658 kb on - strand at 5077.725 kb on + strand at 5077.859 kb on + strand at 5077.859 kb on + strand at 5077.860 kb on - strand at 5077.863 kb on + strand at 5077.863 kb on + strand at 5077.939 kb on + strand, within yebG at 5078.443 kb on + strand, within yebF at 5078.475 kb on + strand at 5078.501 kb on + strand at 5078.740 kb on + strand, within OKFHMN_26370 at 5078.817 kb on + strand at 5078.817 kb on + strand at 5078.818 kb on - strand at 5078.934 kb on + strand at 5079.046 kb on + strand, within yebE at 5079.429 kb on + strand, within yebE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,077,476 - purT OKFHMN_26350 0.80 +0.6 5,077,528 - purT OKFHMN_26350 0.85 +0.9 5,077,531 - purT OKFHMN_26350 0.85 -0.0 5,077,655 - -0.1 5,077,658 - +0.1 5,077,725 + -3.5 5,077,859 + -0.8 5,077,859 + +0.7 5,077,860 - +0.3 5,077,863 + +0.2 5,077,863 + +0.2 5,077,939 + yebG OKFHMN_26355 0.34 +0.4 5,078,443 + yebF OKFHMN_26360 0.69 -0.4 5,078,475 + +0.4 5,078,501 + +0.2 5,078,740 + OKFHMN_26370 0.26 -3.9 5,078,817 + +1.5 5,078,817 + +1.2 5,078,818 - +0.4 5,078,934 + +1.0 5,079,046 + yebE OKFHMN_26375 0.25 +1.6 5,079,429 + yebE OKFHMN_26375 0.83 +1.1
Or see this region's nucleotide sequence