Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25830

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuvrC and pgsA are separated by 56 nucleotidespgsA and Gly_trna are separated by 151 nucleotidesGly_trna and Cys_trna are separated by 53 nucleotidesCys_trna and Leu_trna are separated by 12 nucleotides OKFHMN_25825: uvrC - excinuclease ABC subunit UvrC, at 4,992,128 to 4,993,960 uvrC OKFHMN_25830: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 4,994,017 to 4,994,565 pgsA OKFHMN_25835: Gly_trna - tRNA-Gly(gcc), at 4,994,717 to 4,994,792 _trna OKFHMN_25840: Cys_trna - tRNA-Cys(gca), at 4,994,846 to 4,994,919 _trna OKFHMN_25845: Leu_trna - tRNA-Leu(taa), at 4,994,932 to 4,995,018 _trna Position (kb) 4994 4995Strain fitness (log2 ratio) -3 -2 -1 0 1at 4993.037 kb on + strand, within uvrCat 4993.041 kb on + strand, within uvrCat 4993.333 kb on + strand, within uvrCat 4993.412 kb on - strand, within uvrCat 4993.412 kb on - strand, within uvrCat 4993.498 kb on + strand, within uvrCat 4993.498 kb on + strand, within uvrCat 4993.851 kb on - strandat 4994.683 kb on + strandat 4995.461 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,993,037 + uvrC OKFHMN_25825 0.50 -0.0
4,993,041 + uvrC OKFHMN_25825 0.50 -2.9
4,993,333 + uvrC OKFHMN_25825 0.66 -0.7
4,993,412 - uvrC OKFHMN_25825 0.70 -2.0
4,993,412 - uvrC OKFHMN_25825 0.70 +0.3
4,993,498 + uvrC OKFHMN_25825 0.75 -1.1
4,993,498 + uvrC OKFHMN_25825 0.75 -1.2
4,993,851 - -0.6
4,994,683 + +1.5
4,995,461 + -0.1

Or see this region's nucleotide sequence