Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and tnp-IS3 overlap by 1 nucleotides tnp-IS3 and OKFHMN_25380 are separated by 319 nucleotides OKFHMN_25380 and OKFHMN_25385 are separated by 4 nucleotides OKFHMN_25385 and OKFHMN_25390 are separated by 50 nucleotides OKFHMN_25390 and OKFHMN_25395 are separated by 155 nucleotides OKFHMN_25395 and OKFHMN_25400 are separated by 12 nucleotides
OKFHMN_25370: tnp-IS3 - IS3 family transposase, at 4,916,381 to 4,917,268
tnp-IS3
OKFHMN_25375: tnp-IS3 - IS3 family transposase, at 4,917,268 to 4,917,594
tnp-IS3
OKFHMN_25380: OKFHMN_25380 - lysis protein, at 4,917,914 to 4,918,120
_25380
OKFHMN_25385: OKFHMN_25385 - DUF1327 domain-containing protein, at 4,918,125 to 4,918,469
_25385
OKFHMN_25390: OKFHMN_25390 - SAR-endolysin, at 4,918,520 to 4,919,053
_25390
OKFHMN_25395: OKFHMN_25395 - hypothetical protein, at 4,919,209 to 4,919,391
_25395
OKFHMN_25400: OKFHMN_25400 - hypothetical protein, at 4,919,404 to 4,919,535
_25400
Position (kb) 4918
4919 Strain fitness (log2 ratio) -2
-1
0
1 at 4917.665 kb on + strand at 4917.683 kb on + strand at 4917.683 kb on + strand at 4917.688 kb on - strand at 4917.688 kb on - strand at 4917.688 kb on - strand at 4917.689 kb on + strand at 4917.736 kb on + strand at 4918.699 kb on + strand, within OKFHMN_25390 at 4919.143 kb on - strand at 4919.218 kb on + strand at 4919.218 kb on + strand at 4919.244 kb on + strand, within OKFHMN_25395
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,917,665 + +0.2 4,917,683 + +0.6 4,917,683 + +0.0 4,917,688 - -0.4 4,917,688 - +0.6 4,917,688 - -2.0 4,917,689 + -1.6 4,917,736 + +0.2 4,918,699 + OKFHMN_25390 0.34 -0.2 4,919,143 - +0.5 4,919,218 + -0.3 4,919,218 + -0.2 4,919,244 + OKFHMN_25395 0.19 +0.7
Or see this region's nucleotide sequence