Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24495

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisIE and hisF overlap by 7 nucleotideshisF and hisA overlap by 19 nucleotideshisA and hisH overlap by 1 nucleotideshisH and hisB overlap by 1 nucleotides OKFHMN_24485: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 4,786,504 to 4,787,115 hisIE OKFHMN_24490: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 4,787,109 to 4,787,885 hisF OKFHMN_24495: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 4,787,867 to 4,788,604 hisA OKFHMN_24500: hisH - imidazole glycerol phosphate synthase subunit HisH, at 4,788,604 to 4,789,194 hisH OKFHMN_24505: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 4,789,194 to 4,790,261 hisB Position (kb) 4787 4788 4789Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4786.891 kb on + strand, within hisIEat 4786.895 kb on - strand, within hisIEat 4787.009 kb on + strand, within hisIEat 4787.514 kb on - strand, within hisFat 4788.046 kb on - strand, within hisAat 4788.046 kb on - strand, within hisAat 4788.723 kb on + strand, within hisHat 4788.724 kb on - strand, within hisHat 4788.849 kb on + strand, within hisHat 4789.285 kb on - strandat 4789.304 kb on + strand, within hisBat 4789.343 kb on - strand, within hisBat 4789.534 kb on - strand, within hisB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,786,891 + hisIE OKFHMN_24485 0.63 -2.0
4,786,895 - hisIE OKFHMN_24485 0.64 -1.2
4,787,009 + hisIE OKFHMN_24485 0.83 -4.6
4,787,514 - hisF OKFHMN_24490 0.52 -2.7
4,788,046 - hisA OKFHMN_24495 0.24 -5.1
4,788,046 - hisA OKFHMN_24495 0.24 -3.3
4,788,723 + hisH OKFHMN_24500 0.20 -2.8
4,788,724 - hisH OKFHMN_24500 0.20 -2.0
4,788,849 + hisH OKFHMN_24500 0.41 -2.4
4,789,285 - -4.8
4,789,304 + hisB OKFHMN_24505 0.10 -2.9
4,789,343 - hisB OKFHMN_24505 0.14 -1.8
4,789,534 - hisB OKFHMN_24505 0.32 -0.2

Or see this region's nucleotide sequence