Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides
OKFHMN_24485: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 4,786,504 to 4,787,115
hisIE
OKFHMN_24490: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 4,787,109 to 4,787,885
hisF
OKFHMN_24495: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 4,787,867 to 4,788,604
hisA
OKFHMN_24500: hisH - imidazole glycerol phosphate synthase subunit HisH, at 4,788,604 to 4,789,194
hisH
OKFHMN_24505: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 4,789,194 to 4,790,261
hisB
Position (kb)
4787
4788
4789 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4786.891 kb on + strand, within hisIE at 4786.895 kb on - strand, within hisIE at 4787.009 kb on + strand, within hisIE at 4787.514 kb on - strand, within hisF at 4788.046 kb on - strand, within hisA at 4788.046 kb on - strand, within hisA at 4788.723 kb on + strand, within hisH at 4788.724 kb on - strand, within hisH at 4788.849 kb on + strand, within hisH at 4789.285 kb on - strand at 4789.304 kb on + strand, within hisB at 4789.343 kb on - strand, within hisB at 4789.534 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,786,891 + hisIE OKFHMN_24485 0.63 -2.0 4,786,895 - hisIE OKFHMN_24485 0.64 -1.2 4,787,009 + hisIE OKFHMN_24485 0.83 -4.6 4,787,514 - hisF OKFHMN_24490 0.52 -2.7 4,788,046 - hisA OKFHMN_24495 0.24 -5.1 4,788,046 - hisA OKFHMN_24495 0.24 -3.3 4,788,723 + hisH OKFHMN_24500 0.20 -2.8 4,788,724 - hisH OKFHMN_24500 0.20 -2.0 4,788,849 + hisH OKFHMN_24500 0.41 -2.4 4,789,285 - -4.8 4,789,304 + hisB OKFHMN_24505 0.10 -2.9 4,789,343 - hisB OKFHMN_24505 0.14 -1.8 4,789,534 - hisB OKFHMN_24505 0.32 -0.2
Or see this region's nucleotide sequence