Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24245

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntterY and pphC are separated by 77 nucleotidespphC and yegI overlap by 4 nucleotidesyegI and yegD are separated by 12 nucleotides OKFHMN_24235: terY - Uncharacterized protein YegL, at 4,727,561 to 4,728,220 terY OKFHMN_24240: pphC - Serine/threonine-protein phosphatase 3, at 4,728,298 to 4,728,978 pphC OKFHMN_24245: yegI - protein kinase YegI, at 4,728,975 to 4,730,915 yegI OKFHMN_24250: yegD - molecular chaperone, at 4,730,928 to 4,732,232 yegD Position (kb) 4728 4729 4730 4731Strain fitness (log2 ratio) -3 -2 -1 0 1at 4728.058 kb on + strand, within terYat 4728.333 kb on - strandat 4728.418 kb on + strand, within pphCat 4728.418 kb on + strand, within pphCat 4728.522 kb on + strand, within pphCat 4728.673 kb on + strand, within pphCat 4728.769 kb on - strand, within pphCat 4728.933 kb on + strandat 4728.988 kb on + strandat 4728.988 kb on + strandat 4728.989 kb on - strandat 4728.989 kb on - strandat 4728.989 kb on - strandat 4728.990 kb on + strandat 4728.991 kb on - strandat 4728.992 kb on + strandat 4728.992 kb on + strandat 4729.118 kb on - strandat 4729.303 kb on - strand, within yegIat 4729.332 kb on + strand, within yegIat 4729.332 kb on + strand, within yegIat 4729.420 kb on + strand, within yegIat 4729.652 kb on + strand, within yegIat 4729.707 kb on + strand, within yegIat 4730.003 kb on + strand, within yegIat 4730.059 kb on + strand, within yegIat 4730.146 kb on - strand, within yegIat 4730.223 kb on - strand, within yegIat 4730.334 kb on + strand, within yegIat 4730.334 kb on + strand, within yegIat 4730.344 kb on + strand, within yegIat 4730.860 kb on + strandat 4731.070 kb on + strand, within yegDat 4731.375 kb on - strand, within yegDat 4731.380 kb on - strand, within yegDat 4731.399 kb on + strand, within yegDat 4731.474 kb on - strand, within yegDat 4731.667 kb on - strand, within yegD

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,728,058 + terY OKFHMN_24235 0.75 -0.8
4,728,333 - +0.7
4,728,418 + pphC OKFHMN_24240 0.18 +0.7
4,728,418 + pphC OKFHMN_24240 0.18 +0.2
4,728,522 + pphC OKFHMN_24240 0.33 -0.1
4,728,673 + pphC OKFHMN_24240 0.55 -0.9
4,728,769 - pphC OKFHMN_24240 0.69 +0.3
4,728,933 + -0.5
4,728,988 + -3.0
4,728,988 + +0.8
4,728,989 - -0.1
4,728,989 - -0.2
4,728,989 - +0.6
4,728,990 + -0.6
4,728,991 - -0.1
4,728,992 + +1.2
4,728,992 + +0.2
4,729,118 - -0.0
4,729,303 - yegI OKFHMN_24245 0.17 -0.2
4,729,332 + yegI OKFHMN_24245 0.18 -0.4
4,729,332 + yegI OKFHMN_24245 0.18 -0.2
4,729,420 + yegI OKFHMN_24245 0.23 -0.2
4,729,652 + yegI OKFHMN_24245 0.35 +1.5
4,729,707 + yegI OKFHMN_24245 0.38 -1.7
4,730,003 + yegI OKFHMN_24245 0.53 +0.3
4,730,059 + yegI OKFHMN_24245 0.56 +0.1
4,730,146 - yegI OKFHMN_24245 0.60 -0.9
4,730,223 - yegI OKFHMN_24245 0.64 +0.1
4,730,334 + yegI OKFHMN_24245 0.70 -0.3
4,730,334 + yegI OKFHMN_24245 0.70 +0.2
4,730,344 + yegI OKFHMN_24245 0.71 -0.0
4,730,860 + +0.4
4,731,070 + yegD OKFHMN_24250 0.11 -1.4
4,731,375 - yegD OKFHMN_24250 0.34 +0.0
4,731,380 - yegD OKFHMN_24250 0.35 -0.1
4,731,399 + yegD OKFHMN_24250 0.36 +0.2
4,731,474 - yegD OKFHMN_24250 0.42 +0.4
4,731,667 - yegD OKFHMN_24250 0.57 -0.6

Or see this region's nucleotide sequence