Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mqo and eco are separated by 149 nucleotides eco and OKFHMN_23235 are separated by 133 nucleotides OKFHMN_23235 and OKFHMN_23240 are separated by 51 nucleotides OKFHMN_23240 and napF are separated by 15 nucleotides napF and napD overlap by 11 nucleotides napD and napA overlap by 4 nucleotides
OKFHMN_23225: mqo - malate dehydrogenase (quinone), at 4,533,616 to 4,535,262
mqo
OKFHMN_23230: eco - serine protease inhibitor ecotin, at 4,535,412 to 4,535,900
eco
OKFHMN_23235: OKFHMN_23235 - hypothetical protein, at 4,536,034 to 4,536,198
_23235
OKFHMN_23240: OKFHMN_23240 - Orf 5, at 4,536,250 to 4,536,291
_23240
OKFHMN_23245: napF - ferredoxin-type protein NapF, at 4,536,307 to 4,536,801
napF
OKFHMN_23250: napD - chaperone NapD, at 4,536,791 to 4,537,054
napD
OKFHMN_23255: napA - nitrate reductase catalytic subunit NapA, at 4,537,051 to 4,539,537
napA
Position (kb)
4536
4537 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4535.071 kb on - strand, within mqo at 4535.095 kb on + strand, within mqo at 4535.196 kb on + strand at 4535.196 kb on + strand at 4535.196 kb on + strand at 4535.260 kb on + strand at 4535.260 kb on + strand at 4535.261 kb on - strand at 4535.286 kb on - strand at 4535.286 kb on - strand at 4535.302 kb on - strand at 4535.302 kb on - strand at 4535.351 kb on - strand at 4535.473 kb on - strand, within eco at 4535.489 kb on - strand, within eco at 4535.489 kb on - strand, within eco at 4535.516 kb on - strand, within eco at 4535.542 kb on + strand, within eco at 4535.543 kb on - strand, within eco at 4535.610 kb on - strand, within eco at 4535.877 kb on + strand at 4535.960 kb on - strand at 4536.179 kb on - strand, within OKFHMN_23235 at 4536.214 kb on + strand at 4536.245 kb on + strand at 4536.436 kb on - strand, within napF at 4536.439 kb on - strand, within napF at 4536.524 kb on - strand, within napF at 4536.555 kb on - strand, within napF at 4536.700 kb on + strand, within napF at 4536.714 kb on + strand, within napF at 4536.715 kb on - strand, within napF at 4536.800 kb on - strand at 4536.978 kb on + strand, within napD at 4537.075 kb on - strand at 4537.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,535,071 - mqo OKFHMN_23225 0.88 +1.2 4,535,095 + mqo OKFHMN_23225 0.90 -0.8 4,535,196 + -0.3 4,535,196 + +1.2 4,535,196 + +0.0 4,535,260 + -0.3 4,535,260 + +0.0 4,535,261 - +0.3 4,535,286 - +0.6 4,535,286 - +1.8 4,535,302 - -0.3 4,535,302 - +0.3 4,535,351 - +0.8 4,535,473 - eco OKFHMN_23230 0.12 -0.4 4,535,489 - eco OKFHMN_23230 0.16 -0.7 4,535,489 - eco OKFHMN_23230 0.16 -0.0 4,535,516 - eco OKFHMN_23230 0.21 +0.1 4,535,542 + eco OKFHMN_23230 0.27 -0.1 4,535,543 - eco OKFHMN_23230 0.27 -0.6 4,535,610 - eco OKFHMN_23230 0.40 -0.2 4,535,877 + -2.3 4,535,960 - -1.0 4,536,179 - OKFHMN_23235 0.88 +0.1 4,536,214 + +0.6 4,536,245 + +0.3 4,536,436 - napF OKFHMN_23245 0.26 +0.6 4,536,439 - napF OKFHMN_23245 0.27 +0.9 4,536,524 - napF OKFHMN_23245 0.44 -2.1 4,536,555 - napF OKFHMN_23245 0.50 +0.1 4,536,700 + napF OKFHMN_23245 0.79 -0.1 4,536,714 + napF OKFHMN_23245 0.82 +0.1 4,536,715 - napF OKFHMN_23245 0.82 +0.1 4,536,800 - +0.6 4,536,978 + napD OKFHMN_23250 0.71 +0.4 4,537,075 - -0.3 4,537,168 - +0.1
Or see this region's nucleotide sequence