Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ais and nudI are separated by 38 nucleotides nudI and yfaZ are separated by 278 nucleotides yfaZ and yfaY are separated by 99 nucleotides
OKFHMN_23000: ais - Lipopolysaccharide core heptose(II)-phosphate phosphatase, at 4,478,536 to 4,479,138
ais
OKFHMN_23005: nudI - nucleoside triphosphatase NudI, at 4,479,177 to 4,479,602
nudI
OKFHMN_23010: yfaZ - Outer membrane protein YfaZ, at 4,479,881 to 4,480,423
yfaZ
OKFHMN_23015: yfaY - nicotinamide mononucleotide deamidase-related protein YfaY, at 4,480,523 to 4,481,725
yfaY
Position (kb)
4479
4480
4481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4478.917 kb on - strand, within ais at 4479.027 kb on + strand, within ais at 4479.326 kb on - strand, within nudI at 4479.326 kb on - strand, within nudI at 4479.335 kb on - strand, within nudI at 4479.362 kb on - strand, within nudI at 4479.553 kb on + strand, within nudI at 4479.553 kb on + strand, within nudI at 4479.616 kb on - strand at 4479.719 kb on - strand at 4479.829 kb on - strand at 4479.974 kb on + strand, within yfaZ at 4479.975 kb on - strand, within yfaZ at 4480.042 kb on + strand, within yfaZ at 4480.229 kb on + strand, within yfaZ at 4480.232 kb on + strand, within yfaZ at 4480.232 kb on + strand, within yfaZ at 4480.233 kb on - strand, within yfaZ at 4480.474 kb on + strand at 4480.489 kb on + strand at 4480.491 kb on + strand at 4480.519 kb on - strand at 4480.519 kb on - strand at 4480.542 kb on + strand at 4480.564 kb on - strand at 4480.564 kb on - strand at 4480.632 kb on + strand at 4480.671 kb on + strand, within yfaY at 4480.753 kb on - strand, within yfaY at 4480.851 kb on + strand, within yfaY at 4480.909 kb on + strand, within yfaY at 4480.970 kb on - strand, within yfaY at 4480.994 kb on - strand, within yfaY at 4481.073 kb on + strand, within yfaY at 4481.077 kb on + strand, within yfaY at 4481.125 kb on - strand, within yfaY
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,478,917 - ais OKFHMN_23000 0.63 -2.1 4,479,027 + ais OKFHMN_23000 0.81 -0.7 4,479,326 - nudI OKFHMN_23005 0.35 -0.2 4,479,326 - nudI OKFHMN_23005 0.35 -1.0 4,479,335 - nudI OKFHMN_23005 0.37 -1.0 4,479,362 - nudI OKFHMN_23005 0.43 -1.1 4,479,553 + nudI OKFHMN_23005 0.88 -0.6 4,479,553 + nudI OKFHMN_23005 0.88 +0.1 4,479,616 - +0.1 4,479,719 - -0.8 4,479,829 - +0.3 4,479,974 + yfaZ OKFHMN_23010 0.17 -2.4 4,479,975 - yfaZ OKFHMN_23010 0.17 -2.8 4,480,042 + yfaZ OKFHMN_23010 0.30 -0.3 4,480,229 + yfaZ OKFHMN_23010 0.64 -0.3 4,480,232 + yfaZ OKFHMN_23010 0.65 -0.5 4,480,232 + yfaZ OKFHMN_23010 0.65 +0.0 4,480,233 - yfaZ OKFHMN_23010 0.65 +0.1 4,480,474 + -0.7 4,480,489 + +0.2 4,480,491 + -2.2 4,480,519 - -0.7 4,480,519 - +0.2 4,480,542 + +0.3 4,480,564 - -0.3 4,480,564 - -0.3 4,480,632 + +0.6 4,480,671 + yfaY OKFHMN_23015 0.12 +0.4 4,480,753 - yfaY OKFHMN_23015 0.19 -0.6 4,480,851 + yfaY OKFHMN_23015 0.27 +0.5 4,480,909 + yfaY OKFHMN_23015 0.32 +0.4 4,480,970 - yfaY OKFHMN_23015 0.37 -0.0 4,480,994 - yfaY OKFHMN_23015 0.39 +2.3 4,481,073 + yfaY OKFHMN_23015 0.46 +0.8 4,481,077 + yfaY OKFHMN_23015 0.46 -0.8 4,481,125 - yfaY OKFHMN_23015 0.50 +0.4
Or see this region's nucleotide sequence