Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23010

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntais and nudI are separated by 38 nucleotidesnudI and yfaZ are separated by 278 nucleotidesyfaZ and yfaY are separated by 99 nucleotides OKFHMN_23000: ais - Lipopolysaccharide core heptose(II)-phosphate phosphatase, at 4,478,536 to 4,479,138 ais OKFHMN_23005: nudI - nucleoside triphosphatase NudI, at 4,479,177 to 4,479,602 nudI OKFHMN_23010: yfaZ - Outer membrane protein YfaZ, at 4,479,881 to 4,480,423 yfaZ OKFHMN_23015: yfaY - nicotinamide mononucleotide deamidase-related protein YfaY, at 4,480,523 to 4,481,725 yfaY Position (kb) 4479 4480 4481Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4478.917 kb on - strand, within aisat 4479.027 kb on + strand, within aisat 4479.326 kb on - strand, within nudIat 4479.326 kb on - strand, within nudIat 4479.335 kb on - strand, within nudIat 4479.362 kb on - strand, within nudIat 4479.553 kb on + strand, within nudIat 4479.553 kb on + strand, within nudIat 4479.616 kb on - strandat 4479.719 kb on - strandat 4479.829 kb on - strandat 4479.974 kb on + strand, within yfaZat 4479.975 kb on - strand, within yfaZat 4480.042 kb on + strand, within yfaZat 4480.229 kb on + strand, within yfaZat 4480.232 kb on + strand, within yfaZat 4480.232 kb on + strand, within yfaZat 4480.233 kb on - strand, within yfaZat 4480.474 kb on + strandat 4480.489 kb on + strandat 4480.491 kb on + strandat 4480.519 kb on - strandat 4480.519 kb on - strandat 4480.542 kb on + strandat 4480.564 kb on - strandat 4480.564 kb on - strandat 4480.632 kb on + strandat 4480.671 kb on + strand, within yfaYat 4480.753 kb on - strand, within yfaYat 4480.851 kb on + strand, within yfaYat 4480.909 kb on + strand, within yfaYat 4480.970 kb on - strand, within yfaYat 4480.994 kb on - strand, within yfaYat 4481.073 kb on + strand, within yfaYat 4481.077 kb on + strand, within yfaYat 4481.125 kb on - strand, within yfaY

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,478,917 - ais OKFHMN_23000 0.63 -2.1
4,479,027 + ais OKFHMN_23000 0.81 -0.7
4,479,326 - nudI OKFHMN_23005 0.35 -0.2
4,479,326 - nudI OKFHMN_23005 0.35 -1.0
4,479,335 - nudI OKFHMN_23005 0.37 -1.0
4,479,362 - nudI OKFHMN_23005 0.43 -1.1
4,479,553 + nudI OKFHMN_23005 0.88 -0.6
4,479,553 + nudI OKFHMN_23005 0.88 +0.1
4,479,616 - +0.1
4,479,719 - -0.8
4,479,829 - +0.3
4,479,974 + yfaZ OKFHMN_23010 0.17 -2.4
4,479,975 - yfaZ OKFHMN_23010 0.17 -2.8
4,480,042 + yfaZ OKFHMN_23010 0.30 -0.3
4,480,229 + yfaZ OKFHMN_23010 0.64 -0.3
4,480,232 + yfaZ OKFHMN_23010 0.65 -0.5
4,480,232 + yfaZ OKFHMN_23010 0.65 +0.0
4,480,233 - yfaZ OKFHMN_23010 0.65 +0.1
4,480,474 + -0.7
4,480,489 + +0.2
4,480,491 + -2.2
4,480,519 - -0.7
4,480,519 - +0.2
4,480,542 + +0.3
4,480,564 - -0.3
4,480,564 - -0.3
4,480,632 + +0.6
4,480,671 + yfaY OKFHMN_23015 0.12 +0.4
4,480,753 - yfaY OKFHMN_23015 0.19 -0.6
4,480,851 + yfaY OKFHMN_23015 0.27 +0.5
4,480,909 + yfaY OKFHMN_23015 0.32 +0.4
4,480,970 - yfaY OKFHMN_23015 0.37 -0.0
4,480,994 - yfaY OKFHMN_23015 0.39 +2.3
4,481,073 + yfaY OKFHMN_23015 0.46 +0.8
4,481,077 + yfaY OKFHMN_23015 0.46 -0.8
4,481,125 - yfaY OKFHMN_23015 0.50 +0.4

Or see this region's nucleotide sequence