Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prmB and aroC are separated by 34 nucleotides aroC and mepA are separated by 3 nucleotides mepA and yfcA overlap by 1 nucleotides
OKFHMN_22610: prmB - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,399,776 to 4,400,708
prmB
OKFHMN_22615: aroC - chorismate synthase, at 4,400,743 to 4,401,828
aroC
OKFHMN_22620: mepA - penicillin-insensitive murein endopeptidase, at 4,401,832 to 4,402,656
mepA
OKFHMN_22625: yfcA - putative membrane transporter protein YfcA, at 4,402,656 to 4,403,465
yfcA
Position (kb) 4400
4401
4402 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4399.773 kb on + strand at 4399.773 kb on + strand at 4399.869 kb on - strand at 4399.912 kb on + strand, within prmB at 4399.913 kb on - strand, within prmB at 4399.961 kb on + strand, within prmB at 4399.962 kb on - strand, within prmB at 4400.000 kb on + strand, within prmB at 4400.001 kb on - strand, within prmB at 4400.049 kb on + strand, within prmB at 4400.088 kb on + strand, within prmB at 4400.185 kb on + strand, within prmB at 4400.189 kb on + strand, within prmB at 4400.190 kb on - strand, within prmB at 4400.190 kb on - strand, within prmB at 4400.229 kb on + strand, within prmB at 4400.229 kb on + strand, within prmB at 4400.260 kb on + strand, within prmB at 4400.264 kb on + strand, within prmB at 4400.264 kb on + strand, within prmB at 4400.403 kb on + strand, within prmB at 4400.403 kb on + strand, within prmB at 4400.420 kb on + strand, within prmB at 4400.526 kb on + strand, within prmB at 4400.545 kb on + strand, within prmB at 4400.575 kb on + strand, within prmB at 4400.575 kb on + strand, within prmB at 4400.582 kb on + strand, within prmB at 4400.706 kb on + strand at 4400.907 kb on + strand, within aroC at 4400.908 kb on - strand, within aroC at 4400.967 kb on + strand, within aroC at 4401.026 kb on - strand, within aroC at 4401.068 kb on - strand, within aroC at 4401.308 kb on + strand, within aroC at 4401.609 kb on + strand, within aroC at 4401.928 kb on - strand, within mepA at 4401.963 kb on + strand, within mepA at 4402.077 kb on + strand, within mepA at 4402.259 kb on + strand, within mepA at 4402.467 kb on - strand, within mepA at 4402.679 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,399,773 + +0.1 4,399,773 + -0.7 4,399,869 - -1.7 4,399,912 + prmB OKFHMN_22610 0.15 -0.2 4,399,913 - prmB OKFHMN_22610 0.15 +2.3 4,399,961 + prmB OKFHMN_22610 0.20 -0.4 4,399,962 - prmB OKFHMN_22610 0.20 +0.4 4,400,000 + prmB OKFHMN_22610 0.24 -1.5 4,400,001 - prmB OKFHMN_22610 0.24 -1.3 4,400,049 + prmB OKFHMN_22610 0.29 -0.9 4,400,088 + prmB OKFHMN_22610 0.33 -0.5 4,400,185 + prmB OKFHMN_22610 0.44 -2.2 4,400,189 + prmB OKFHMN_22610 0.44 -0.1 4,400,190 - prmB OKFHMN_22610 0.44 -0.9 4,400,190 - prmB OKFHMN_22610 0.44 -0.7 4,400,229 + prmB OKFHMN_22610 0.49 -1.8 4,400,229 + prmB OKFHMN_22610 0.49 -0.4 4,400,260 + prmB OKFHMN_22610 0.52 -0.2 4,400,264 + prmB OKFHMN_22610 0.52 +0.5 4,400,264 + prmB OKFHMN_22610 0.52 -0.1 4,400,403 + prmB OKFHMN_22610 0.67 -0.9 4,400,403 + prmB OKFHMN_22610 0.67 -0.8 4,400,420 + prmB OKFHMN_22610 0.69 +1.2 4,400,526 + prmB OKFHMN_22610 0.80 +0.5 4,400,545 + prmB OKFHMN_22610 0.82 -1.2 4,400,575 + prmB OKFHMN_22610 0.86 +0.9 4,400,575 + prmB OKFHMN_22610 0.86 +0.3 4,400,582 + prmB OKFHMN_22610 0.86 +0.2 4,400,706 + +0.5 4,400,907 + aroC OKFHMN_22615 0.15 -4.4 4,400,908 - aroC OKFHMN_22615 0.15 -2.9 4,400,967 + aroC OKFHMN_22615 0.21 -4.0 4,401,026 - aroC OKFHMN_22615 0.26 -3.7 4,401,068 - aroC OKFHMN_22615 0.30 -5.3 4,401,308 + aroC OKFHMN_22615 0.52 -4.6 4,401,609 + aroC OKFHMN_22615 0.80 -4.0 4,401,928 - mepA OKFHMN_22620 0.12 +0.2 4,401,963 + mepA OKFHMN_22620 0.16 +0.3 4,402,077 + mepA OKFHMN_22620 0.30 +0.4 4,402,259 + mepA OKFHMN_22620 0.52 -2.0 4,402,467 - mepA OKFHMN_22620 0.77 +0.1 4,402,679 + -0.0
Or see this region's nucleotide sequence