Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsI and ptsH are separated by 44 nucleotides ptsH and cysK are separated by 383 nucleotides cysK and cysZ are separated by 184 nucleotides
OKFHMN_22190: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 4,314,395 to 4,316,122
ptsI
OKFHMN_22195: ptsH - phosphocarrier protein Hpr, at 4,316,167 to 4,316,424
ptsH
OKFHMN_22200: cysK - cysteine synthase A, at 4,316,808 to 4,317,779
cysK
OKFHMN_22205: cysZ - sulfate transporter CysZ, at 4,317,964 to 4,318,725
cysZ
Position (kb)
4316
4317
4318 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4315.891 kb on + strand, within ptsI at 4315.892 kb on - strand, within ptsI at 4315.895 kb on - strand, within ptsI at 4316.134 kb on - strand at 4316.134 kb on - strand at 4316.326 kb on - strand, within ptsH at 4316.347 kb on - strand, within ptsH at 4316.442 kb on + strand at 4316.496 kb on - strand at 4316.496 kb on - strand at 4316.542 kb on + strand at 4316.597 kb on + strand at 4316.598 kb on - strand at 4316.666 kb on - strand at 4316.683 kb on + strand at 4317.077 kb on + strand, within cysK at 4317.185 kb on + strand, within cysK at 4317.213 kb on - strand, within cysK at 4317.263 kb on + strand, within cysK at 4317.264 kb on - strand, within cysK at 4317.268 kb on - strand, within cysK at 4317.368 kb on - strand, within cysK at 4317.428 kb on + strand, within cysK at 4317.489 kb on - strand, within cysK at 4317.540 kb on + strand, within cysK at 4317.541 kb on - strand, within cysK at 4317.544 kb on + strand, within cysK at 4317.544 kb on + strand, within cysK at 4317.545 kb on - strand, within cysK at 4317.545 kb on - strand, within cysK at 4317.545 kb on - strand, within cysK at 4317.545 kb on - strand, within cysK at 4317.545 kb on - strand, within cysK at 4317.656 kb on + strand, within cysK at 4317.657 kb on - strand, within cysK at 4317.657 kb on - strand, within cysK at 4317.747 kb on - strand at 4317.759 kb on - strand at 4317.790 kb on + strand at 4317.791 kb on - strand at 4317.809 kb on + strand at 4317.810 kb on - strand at 4317.818 kb on + strand at 4317.818 kb on + strand at 4317.819 kb on - strand at 4317.819 kb on - strand at 4317.823 kb on + strand at 4317.856 kb on + strand at 4317.857 kb on - strand at 4317.862 kb on - strand at 4317.865 kb on + strand at 4317.865 kb on + strand at 4317.867 kb on + strand at 4317.914 kb on + strand at 4317.992 kb on + strand at 4318.038 kb on + strand at 4318.051 kb on - strand, within cysZ at 4318.051 kb on - strand, within cysZ at 4318.068 kb on + strand, within cysZ at 4318.242 kb on + strand, within cysZ at 4318.243 kb on - strand, within cysZ at 4318.243 kb on - strand, within cysZ at 4318.289 kb on + strand, within cysZ at 4318.289 kb on + strand, within cysZ at 4318.408 kb on + strand, within cysZ at 4318.469 kb on - strand, within cysZ at 4318.505 kb on + strand, within cysZ at 4318.506 kb on - strand, within cysZ at 4318.506 kb on - strand, within cysZ at 4318.506 kb on - strand, within cysZ at 4318.519 kb on + strand, within cysZ at 4318.536 kb on - strand, within cysZ at 4318.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,315,891 + ptsI OKFHMN_22190 0.87 +1.7 4,315,892 - ptsI OKFHMN_22190 0.87 -0.5 4,315,895 - ptsI OKFHMN_22190 0.87 -0.8 4,316,134 - -1.6 4,316,134 - +1.8 4,316,326 - ptsH OKFHMN_22195 0.62 -0.9 4,316,347 - ptsH OKFHMN_22195 0.70 +0.5 4,316,442 + +0.3 4,316,496 - +0.9 4,316,496 - +0.1 4,316,542 + -0.4 4,316,597 + +0.2 4,316,598 - +1.2 4,316,666 - +0.5 4,316,683 + -1.3 4,317,077 + cysK OKFHMN_22200 0.28 +0.3 4,317,185 + cysK OKFHMN_22200 0.39 -2.3 4,317,213 - cysK OKFHMN_22200 0.42 -3.2 4,317,263 + cysK OKFHMN_22200 0.47 -0.2 4,317,264 - cysK OKFHMN_22200 0.47 -2.1 4,317,268 - cysK OKFHMN_22200 0.47 -2.0 4,317,368 - cysK OKFHMN_22200 0.58 -3.2 4,317,428 + cysK OKFHMN_22200 0.64 -3.4 4,317,489 - cysK OKFHMN_22200 0.70 -2.4 4,317,540 + cysK OKFHMN_22200 0.75 -3.7 4,317,541 - cysK OKFHMN_22200 0.75 -2.4 4,317,544 + cysK OKFHMN_22200 0.76 -1.6 4,317,544 + cysK OKFHMN_22200 0.76 -3.0 4,317,545 - cysK OKFHMN_22200 0.76 -4.5 4,317,545 - cysK OKFHMN_22200 0.76 -1.1 4,317,545 - cysK OKFHMN_22200 0.76 -4.4 4,317,545 - cysK OKFHMN_22200 0.76 -1.1 4,317,545 - cysK OKFHMN_22200 0.76 +1.1 4,317,656 + cysK OKFHMN_22200 0.87 -3.3 4,317,657 - cysK OKFHMN_22200 0.87 -5.0 4,317,657 - cysK OKFHMN_22200 0.87 -1.0 4,317,747 - -1.0 4,317,759 - -1.6 4,317,790 + -1.6 4,317,791 - -2.1 4,317,809 + -3.3 4,317,810 - -0.5 4,317,818 + -4.5 4,317,818 + -3.1 4,317,819 - -1.6 4,317,819 - -0.9 4,317,823 + -1.0 4,317,856 + -2.4 4,317,857 - -0.4 4,317,862 - -0.4 4,317,865 + -1.4 4,317,865 + -1.3 4,317,867 + +0.0 4,317,914 + +0.0 4,317,992 + -0.7 4,318,038 + +0.4 4,318,051 - cysZ OKFHMN_22205 0.11 +0.7 4,318,051 - cysZ OKFHMN_22205 0.11 -0.2 4,318,068 + cysZ OKFHMN_22205 0.14 -0.7 4,318,242 + cysZ OKFHMN_22205 0.36 +0.4 4,318,243 - cysZ OKFHMN_22205 0.37 -0.1 4,318,243 - cysZ OKFHMN_22205 0.37 +0.1 4,318,289 + cysZ OKFHMN_22205 0.43 +0.3 4,318,289 + cysZ OKFHMN_22205 0.43 +2.0 4,318,408 + cysZ OKFHMN_22205 0.58 +0.1 4,318,469 - cysZ OKFHMN_22205 0.66 +0.0 4,318,505 + cysZ OKFHMN_22205 0.71 +0.6 4,318,506 - cysZ OKFHMN_22205 0.71 -0.5 4,318,506 - cysZ OKFHMN_22205 0.71 +0.4 4,318,506 - cysZ OKFHMN_22205 0.71 +1.5 4,318,519 + cysZ OKFHMN_22205 0.73 +0.4 4,318,536 - cysZ OKFHMN_22205 0.75 -0.1 4,318,682 + -1.2
Or see this region's nucleotide sequence