Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21740

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisS and yfgM are separated by 17 nucleotidesyfgM and bamB are separated by 10 nucleotidesbamB and der are separated by 117 nucleotides OKFHMN_21730: hisS - histidine--tRNA ligase, at 4,217,863 to 4,219,137 hisS OKFHMN_21735: yfgM - ancillary SecYEG translocon subunit YfgM, at 4,219,155 to 4,219,775 yfgM OKFHMN_21740: bamB - outer membrane protein assembly factor BamB, at 4,219,786 to 4,220,964 bamB OKFHMN_21745: der - ribosome biogenesis GTPase Der, at 4,221,082 to 4,222,554 der Position (kb) 4219 4220 4221Strain fitness (log2 ratio) -2 -1 0 1at 4219.106 kb on + strandat 4219.196 kb on + strandat 4219.406 kb on + strand, within yfgMat 4219.688 kb on + strand, within yfgMat 4219.874 kb on + strandat 4220.002 kb on + strand, within bamBat 4220.024 kb on + strand, within bamBat 4220.114 kb on + strand, within bamBat 4220.297 kb on + strand, within bamBat 4220.573 kb on - strand, within bamBat 4220.782 kb on + strand, within bamBat 4220.782 kb on + strand, within bamBat 4220.784 kb on + strand, within bamBat 4220.874 kb on + strandat 4220.947 kb on + strandat 4220.955 kb on + strandat 4221.039 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,219,106 + +1.3
4,219,196 + -0.2
4,219,406 + yfgM OKFHMN_21735 0.40 -0.5
4,219,688 + yfgM OKFHMN_21735 0.86 -1.9
4,219,874 + -1.5
4,220,002 + bamB OKFHMN_21740 0.18 -0.8
4,220,024 + bamB OKFHMN_21740 0.20 -2.6
4,220,114 + bamB OKFHMN_21740 0.28 -2.4
4,220,297 + bamB OKFHMN_21740 0.43 -1.2
4,220,573 - bamB OKFHMN_21740 0.67 -0.1
4,220,782 + bamB OKFHMN_21740 0.84 -2.0
4,220,782 + bamB OKFHMN_21740 0.84 -1.9
4,220,784 + bamB OKFHMN_21740 0.85 -1.1
4,220,874 + -1.6
4,220,947 + -0.3
4,220,955 + -0.6
4,221,039 + -0.9

Or see this region's nucleotide sequence