Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21425

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntera and recO are separated by 11 nucleotidesrecO and pdxJ are separated by 11 nucleotidespdxJ and acpS overlap by 1 nucleotidesacpS and shoB are separated by 421 nucleotides OKFHMN_21415: era - GTPase Era, at 4,150,809 to 4,151,714 era OKFHMN_21420: recO - DNA repair protein RecO, at 4,151,726 to 4,152,454 recO OKFHMN_21425: pdxJ - pyridoxine 5'-phosphate synthase, at 4,152,466 to 4,153,197 pdxJ OKFHMN_21430: acpS - Holo-[acyl-carrier-protein] synthase, at 4,153,197 to 4,153,577 acpS OKFHMN_21440: shoB - Small toxic protein ShoB, at 4,153,999 to 4,154,079 shoB Position (kb) 4152 4153 4154Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4151.898 kb on + strand, within recOat 4152.215 kb on + strand, within recOat 4152.288 kb on + strand, within recOat 4152.289 kb on - strand, within recOat 4152.424 kb on + strandat 4152.446 kb on + strandat 4152.602 kb on + strand, within pdxJat 4152.624 kb on + strand, within pdxJat 4152.767 kb on + strand, within pdxJat 4153.064 kb on + strand, within pdxJat 4153.582 kb on - strandat 4153.604 kb on - strandat 4153.620 kb on + strandat 4153.709 kb on - strandat 4153.750 kb on + strandat 4153.823 kb on - strandat 4153.924 kb on + strandat 4153.953 kb on + strandat 4154.145 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,151,898 + recO OKFHMN_21420 0.24 -1.0
4,152,215 + recO OKFHMN_21420 0.67 -0.2
4,152,288 + recO OKFHMN_21420 0.77 -1.6
4,152,289 - recO OKFHMN_21420 0.77 -1.4
4,152,424 + -0.9
4,152,446 + -0.1
4,152,602 + pdxJ OKFHMN_21425 0.19 -2.5
4,152,624 + pdxJ OKFHMN_21425 0.22 -4.2
4,152,767 + pdxJ OKFHMN_21425 0.41 -2.4
4,153,064 + pdxJ OKFHMN_21425 0.82 -1.8
4,153,582 - -0.2
4,153,604 - +0.0
4,153,620 + +0.9
4,153,709 - -0.1
4,153,750 + +0.6
4,153,823 - +0.2
4,153,924 + +0.8
4,153,953 + +0.1
4,154,145 - -3.0

Or see this region's nucleotide sequence