Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21420

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnc and era overlap by 4 nucleotidesera and recO are separated by 11 nucleotidesrecO and pdxJ are separated by 11 nucleotidespdxJ and acpS overlap by 1 nucleotides OKFHMN_21410: rnc - ribonuclease III, at 4,150,132 to 4,150,812 rnc OKFHMN_21415: era - GTPase Era, at 4,150,809 to 4,151,714 era OKFHMN_21420: recO - DNA repair protein RecO, at 4,151,726 to 4,152,454 recO OKFHMN_21425: pdxJ - pyridoxine 5'-phosphate synthase, at 4,152,466 to 4,153,197 pdxJ OKFHMN_21430: acpS - Holo-[acyl-carrier-protein] synthase, at 4,153,197 to 4,153,577 acpS Position (kb) 4151 4152 4153Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4151.898 kb on + strand, within recOat 4152.215 kb on + strand, within recOat 4152.288 kb on + strand, within recOat 4152.289 kb on - strand, within recOat 4152.424 kb on + strandat 4152.446 kb on + strandat 4152.602 kb on + strand, within pdxJat 4152.624 kb on + strand, within pdxJat 4152.767 kb on + strand, within pdxJat 4153.064 kb on + strand, within pdxJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,151,898 + recO OKFHMN_21420 0.24 -1.0
4,152,215 + recO OKFHMN_21420 0.67 -0.2
4,152,288 + recO OKFHMN_21420 0.77 -1.6
4,152,289 - recO OKFHMN_21420 0.77 -1.4
4,152,424 + -0.9
4,152,446 + -0.1
4,152,602 + pdxJ OKFHMN_21425 0.19 -2.5
4,152,624 + pdxJ OKFHMN_21425 0.22 -4.2
4,152,767 + pdxJ OKFHMN_21425 0.41 -2.4
4,153,064 + pdxJ OKFHMN_21425 0.82 -1.8

Or see this region's nucleotide sequence